Algorithm-Driven Chromatographic Method for Prostaglandin Isomer Identification via Tandem Mass Spectrometry

Hondo, Toshinobu; Miyake, Yumi; Toyoda, Michisato

Journal of the American Society for Mass Spectrometry, 2025, 36(10), 2267-2275

Number of Access:6（2025-11-25 15:43 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/103013

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Title
Algorithm-Driven Chromatographic Method for Prostaglandin Isomer Identification via Tandem Mass Spectrometry
Creator
NRID
1000090723924 1000090723924
ORCID
0000-0003-0062-1619 0000-0003-0062-1619
researchmap permalink
toshi-hondo toshi-hondo
Scopus Author ID
56641101900 56641101900
CreatorName
Hondo, Toshinobu
ORCID
0009-0001-6057-3885 0009-0001-6057-3885
researchmap permalink
21028368 21028368
Scopus Author ID
57216830359 57216830359
CreatorName
Miyake, Yumi
NRID
1000080283828 1000080283828
ORCID
0000-0001-5950-6039 0000-0001-5950-6039
researchmap permalink
toyodam toyodam
Scopus Author ID
7201358693 7201358693
CreatorName
Toyoda, Michisato
Description
Hondo T., Miyake Y., Toyoda M.. Algorithm-Driven Chromatographic Method for Prostaglandin Isomer Identification via Tandem Mass Spectrometry. Journal of the American Society for Mass Spectrometry 36, 2267 (2025); https://doi.org/10.1021/jasms.5c00231.
Abstract
This study explores the computational isolation of prostaglandin (PG) isomers, specifically PG E2(PGE2) and D2(PGD2), to enhance method development efficiency and provide insights into their retention behavior during supercritical fluid extraction (SFE) combined with supercritical fluid chromatography (SFC)-tandem mass spectrometry (MS/MS). Although PGE2and PGD2are positional isomers that yield identical product ions in MS/MS, they serve distinct biological roles. This research illustrates the efficacy of selected reaction monitoring (SRM)-based techniques for differentiating coeluting isomers. Despite the challenges posed by baseline resolution, simplified computational methods successfully distinguished between PGE2and PGD2, demonstrating the potential for high-throughput PG analysis without the necessity for complete chromatographic peak resolution. By employing least-squares estimation to solve a linear system, the abundance ratio of PGE2to PGD2was derived from intensity ratios across four SRM transitions, achieving precise quantification even with poorly resolved SFC peaks. The study highlights critical factors affecting PG retention, such as the choice of the stationary phase, temperature regulation, and reduction of stainless steel interactions, which can diminish signal intensity. A significant observation is the concentration-dependent suppression effect of the entrainer when interacting with the hepatocyte matrix, underscoring the importance of effective matrix management in SFE/SFC-MS/MS. These findings advance the development of a robust, high-throughput analytical platform for PG quantification and lipidomics research applications.
Publisher
American Chemical Society
Source Title
Journal of the American Society for Mass Spectrometry
Volume (Issue)
36(10)
Page
2267-2275
Date of Issued
2025-09-08
Language
English
Handle URL
https://hdl.handle.net/11094/103013
PISSN
10440305
eISSN
18791123
Relation.isVersionOf
DOI (Publisher Version)
https://doi.org/10.1021/jasms.5c00231
Access Rights
embargoed access
CopyRight
This document is the Accepted Manuscript version of a Published Work that appeared in final form in Journal of the American Society for Mass Spectrometry, © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://doi.org/10.1021/jasms.5c00231
The full-text file will be made open to the public on 8 September 2026 in accordance with the publisher's policy.
oaire:version
Accepted Manuscript
Category
学術雑誌論文 Journal Article