Development of starch-based support material alternately extruded with gelatin-based bioinks for 3D bioprinting application

Prasetyaningrum, Pekik Wiji; Mubarok, Wildan; Kotani, Takashi et al.

Bioprinting, 2025, 51, e00439

Number of Access:273（2026-04-04 23:46 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/103286

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Title
Development of starch-based support material alternately extruded with gelatin-based bioinks for 3D bioprinting application
Creator
CreatorName
Prasetyaningrum, Pekik Wiji
NRID
1000080975433 1000080975433
ORCID
0000-0003-2545-1206 0000-0003-2545-1206
researchmap permalink
wildanmubarok wildanmubarok
Scopus Author ID
57208440063 57208440063
CreatorName
Mubarok, Wildan
CreatorName
Kotani, Takashi
NRID
1000020359938 1000020359938
ORCID
0000-0002-1041-4798 0000-0002-1041-4798
researchmap permalink
sakaishinji sakaishinji
Scopus Author ID
35450082900 35450082900
CreatorName
Sakai, Shinji
Subject
3D bioprinting
Gelatin
Horseradish peroxidase
Starch
Stem cells
Description
Prasetyaningrum P.W., Mubarok W., Kotani T., et al. Development of starch-based support material alternately extruded with gelatin-based bioinks for 3D bioprinting application. Bioprinting 51, e00439 (2025); https://doi.org/10.1016/j.bprint.2025.e00439.
Abstract
The use of support materials is crucial for the 3D bioprinting of low-viscosity bioinks, which yield soft hydrogel constructs susceptible to deformation under their weight. In this study, we developed a starch-based support material that provides structural support during printing and supplies hydrogen peroxide (H₂O₂), for printing cell-laden constructs from low-viscosity bioinks (4.4–53.1 mPa s at 1 s⁻¹ shear rate) composed of a gelatin derivative possessing phenolic hydroxyl moieties (gelatin-Ph), horseradish peroxidase (HRP), and cells. Importantly, the support material can be selectively and gently removed using α-amylase, a biocompatible enzyme, without harming the construct or encapsulated cells, which is a significant advancement over conventional methods of removing support systems. 3D constructs were fabricated by alternately extruding bioinks containing 5.0 w/v% gelatin-Ph and 10 U/mL HRP with a support material consisting of 16.7 w/w% starch and 10 mM H₂O₂. Immortalized human bone marrow-derived mesenchymal stem cells encapsulated within the constructs showed >80 % viability after printing and exhibited an elongated morphology and proliferation, while maintaining their stemness over 14 days of culture. The cells underwent osteogenic differentiation when cultured in a differentiation medium, as evidenced by the calcium deposition, alkaline phosphatase activity, and expression of osteogenic genes, demonstrating the potential of the proposed approach for tissue-engineering applications.
Publisher
Elsevier B.V.
Source Title
Bioprinting
Volume (Issue)
51
Page
e00439
Date of Issued
2025-09-20
Language
English
Handle URL
https://hdl.handle.net/11094/103286
PISSN
24058866
Relation.isIdenticalTo
DOI (Publisher Version)
https://doi.org/10.1016/j.bprint.2025.e00439
Access Rights
open access
CopyRight
This article is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
oaire:version
Version of Record
Category
学術雑誌論文 Journal Article