Label-free monitoring of crystalline chitin hydrolysis by chitinase based on Raman spectroscopy

Ando, Jun; Kawagoe, Hiroyuki; Nakamura, Akihiko; Iino, Ryota; Fujita, Katsumasa

doi:https://doi.org/10.1039/d1an00581b

Label-free monitoring of crystalline chitin hydrolysis by chitinase based on Raman spectroscopy

Ando, Jun; Kawagoe, Hiroyuki; Nakamura, Akihiko et al.

Analyst, 2021, 146(12), 4087-4094

Number of Access:133（2026-02-20 11:11 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/103308

Link to primary information

File	Format	Terms of use	Size	Views	Date.Available	Description	information
Analyst_146_12_4087	pdf	None	2.37 MB	21	2025.12.05

Item Information

Output File Export EndNote Basic Export Mendeley

Title	Label-free monitoring of crystalline chitin hydrolysis by chitinase based on Raman spectroscopy
Creator	NRID 1000040623369 1000040623369 CreatorName Ando, Jun
	CreatorName Kawagoe, Hiroyuki
	NRID 1000020752968 1000020752968 CreatorName Nakamura, Akihiko
	NRID 1000070403003 1000070403003 CreatorName Iino, Ryota
	NRID 1000080362664 1000080362664 ORCID 0000-0002-2284-375X 0000-0002-2284-375X researchmap permalink read0089911 read0089911 Scopus Author ID 7404058829 7404058829 CreatorName Fujita, Katsumasa
Description	Reproduced from Analyst 146, 4087 (2021) with permission from the Royal Society of Chemistry.
Abstract	We demonstrate a method for label-free monitoring of hydrolytic activity of crystalline-chitin-degrading enzyme, chitinase, by means of Raman spectroscopy. We found that crystalline chitin exhibited a characteristic Raman peak at 2995 cm⁻¹, which did not appear in the reaction product, N,N′-diacetylchitobiose. We used this Raman peak as a marker of crystalline chitin degradation to monitor the hydrolytic activity of chitinase. When the crystalline chitin suspension and chitinase were mixed together, the peak intensity of crystalline chitin at 2995 cm⁻¹ was linearly decreased depending on incubation time. The decrease in peak intensity was inversely correlated with the increase in the amount of released N,N′-diacetylchitobiose, which was measured by conventional colorimetric assay with alkaline ferricyanide. Our result, presented here, provides a new method for simple, in situ, and label-free monitoring of enzymatic activity of chitinase.
Publisher	Royal Society of Chemistry
Source Title	Analyst
Volume (Issue)	146(12)
Page	4087-4094
Date of Issued	2021-05-27
Language	English
Handle URL	https://hdl.handle.net/11094/103308
PISSN	00032654
eISSN	13645528
Relation.isVersionOf	DOI (Publisher Version) https://doi.org/10.1039/d1an00581b
Access Rights	open access
oaire:version	Accepted Manuscript
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCMI Type	Text
DCTERMS.bibliographicCitation	Analyst. 2021, 146(12), p. 4087-4094
DC.title	Label-free monitoring of crystalline chitin hydrolysis by chitinase based on Raman spectroscopy
DC.creator	Ando, Jun
	Kawagoe, Hiroyuki
	Nakamura, Akihiko
	Iino, Ryota
	Fujita, Katsumasa
DC.publisher	Royal Society of Chemistry
DC.language' scheme='DCTERMS.RFC1766	English
DC.type' scheme='DCTERMS.DCMIType	Text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2021-05-27
DC.identifier	https://doi.org/10.1039/d1an00581b
DC.description	Reproduced from Analyst 146, 4087 (2021) with permission from the Royal Society of Chemistry.
DCTERMS.abstract	We demonstrate a method for label-free monitoring of hydrolytic activity of crystalline-chitin-degrading enzyme, chitinase, by means of Raman spectroscopy. We found that crystalline chitin exhibited a characteristic Raman peak at 2995 cm⁻¹, which did not appear in the reaction product, N,N′-diacetylchitobiose. We used this Raman peak as a marker of crystalline chitin degradation to monitor the hydrolytic activity of chitinase. When the crystalline chitin suspension and chitinase were mixed together, the peak intensity of crystalline chitin at 2995 cm⁻¹ was linearly decreased depending on incubation time. The decrease in peak intensity was inversely correlated with the increase in the amount of released N,N′-diacetylchitobiose, which was measured by conventional colorimetric assay with alkaline ferricyanide. Our result, presented here, provides a new method for simple, in situ, and label-free monitoring of enzymatic activity of chitinase.
DC.format	103308
citation_title	Label-free monitoring of crystalline chitin hydrolysis by chitinase based on Raman spectroscopy
citation_author	Ando, Jun
	Kawagoe, Hiroyuki
	Nakamura, Akihiko
	Iino, Ryota
	Fujita, Katsumasa
citation_publisher	Royal Society of Chemistry
citation_language	English
citation_date	2021-05-27
citation_journal_title	Analyst
citation_volume	146
citation_issue	12
citation_firstpage	4087
citation_lastpage	4094
citation_doi	https://doi.org/10.1039/d1an00581b
citation_public_url	https://hdl.handle.net/11094/103308

Label-free monitoring of crystalline chitin hydrolysis by chitinase based on Raman spectroscopy

Link to primary information

Item Information

Show details