Quantitative drug dynamics visualized by alkyne-tagged plasmonic-enhanced Raman microscopy

Koike, Kota; Bando, Kazuki; Ando, Jun et al.

ACS Nano, 2020, 14(11), 15032-15041

Number of Access:188（2026-02-18 11:34 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/103310

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Title
Quantitative drug dynamics visualized by alkyne-tagged plasmonic-enhanced Raman microscopy
Creator
CreatorName
Koike, Kota
NRID
1000040795952 1000040795952
researchmap permalink
kazukibando kazukibando
Scopus Author ID
11540437400 11540437400
CreatorName
Bando, Kazuki
NRID
1000040623369 1000040623369
CreatorName
Ando, Jun
NRID
1000030596599 1000030596599
CreatorName
Yamakoshi, Hiroyuki
CreatorName
Terayama, Naoki
NRID
1000020415243 1000020415243
CreatorName
Dodo, Kosuke
NRID
1000010362592 1000010362592
ORCID
0000-0002-7835-199X 0000-0002-7835-199X
researchmap permalink
read0089935 read0089935
Scopus Author ID
55262815400 55262815400
CreatorName
Smith, Nicholas Isaac
NRID
1000060192142 1000060192142
CreatorName
Sodeoka, Mikiko
NRID
1000080362664 1000080362664
ORCID
0000-0002-2284-375X 0000-0002-2284-375X
researchmap permalink
read0089911 read0089911
Scopus Author ID
7404058829 7404058829
CreatorName
Fujita, Katsumasa
Subject
Drug imaging
Gold nanoparticle
Raman microscopy
Small molecule
Surface-enhanced Raman scattering
Description
This document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS Nano 14, 15032 (2020), © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://doi.org/10.1021/acsnano.0c05010.
Abstract
Visualizing live-cell uptake of small-molecule drugs is paramount for drug development and pharmaceutical sciences. Bioorthogonal imaging with click chemistry has made significant contributions to the field, visualizing small molecules in cells. Furthermore, recent developments in Raman microscopy, including stimulated Raman scattering (SRS) microscopy, have realized direct visualization of alkyne-tagged small-molecule drugs in live cells. However, Raman and SRS microscopy still suffer from limited detection sensitivity with low concentration molecules for observing temporal dynamics of drug uptake. Here, we demonstrate the combination of alkyne-tag and surface-enhanced Raman scattering (SERS) microscopy for the real-time monitoring of drug uptake in live cells. Gold nanoparticles are introduced into lysosomes of live cells by endocytosis and work as SERS probes. Raman signals of alkynes can be boosted by enhanced electric fields generated by plasmon resonance of gold nanoparticles when alkyne-tagged small molecules are colocalized with the nanoparticles. With time-lapse 3D SERS imaging, this technique allows us to investigate drug uptake by live cells with different chemical and physical conditions. We also perform quantitative evaluation of the uptake speed at the single-cell level using digital SERS counting under different quantities of drug molecules and temperature conditions. Our results illustrate that alkyne-tag SERS microscopy has a potential to be an alternative bioorthogonal imaging technique to investigate temporal dynamics of small-molecule uptake of live cells for pharmaceutical research.
Publisher
American Chemical Society
Source Title
ACS Nano
Volume (Issue)
14(11)
Page
15032-15041
Date of Issued
2020-10-20
Language
English
Handle URL
https://hdl.handle.net/11094/103310
PISSN
19360851
eISSN
1936086X
Relation.isVersionOf
DOI (Publisher Version)
https://doi.org/10.1021/acsnano.0c05010
Access Rights
open access
oaire:version
Accepted Manuscript
Category
学術雑誌論文 Journal Article