Nonlinear Scattering of Near-Infrared Light for Imaging Plasmonic Nanoparticles in Deep Tissue

Nishida, Kentaro; Deka, Gitanjal; Smith, Nicholas Isaac; Chu, Shi Wei; Fujita, Katsumasa

doi:https://doi.org/10.1021/acsphotonics.0c00607

Nonlinear Scattering of Near-Infrared Light for Imaging Plasmonic Nanoparticles in Deep Tissue

Nishida, Kentaro; Deka, Gitanjal; Smith, Nicholas Isaac et al.

ACS Photonics, 2020, 7(8), 2139-2146

Number of Access:115（2026-02-18 16:18 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/103311

Link to primary information

File	Format	Terms of use	Size	Views	Date.Available	Description	information
ACSPhotonics_7_8_2139	pdf	None	1.60 MB	31	2025.12.05

Item Information

Output File Export EndNote Basic Export Mendeley

Title	Nonlinear Scattering of Near-Infrared Light for Imaging Plasmonic Nanoparticles in Deep Tissue
Creator	CreatorName Nishida, Kentaro
	CreatorName Deka, Gitanjal
	NRID 1000010362592 1000010362592 ORCID 0000-0002-7835-199X 0000-0002-7835-199X researchmap permalink read0089935 read0089935 Scopus Author ID 55262815400 55262815400 CreatorName Smith, Nicholas Isaac
	CreatorName Chu, Shi Wei
	NRID 1000080362664 1000080362664 ORCID 0000-0002-2284-375X 0000-0002-2284-375X researchmap permalink read0089911 read0089911 Scopus Author ID 7404058829 7404058829 CreatorName Fujita, Katsumasa
Subject	deep tissue imaging
	gold nanoparticle
	near-infrared
	nonlinear
	plasmonics
	super-resolution microscopy
Description	This document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS Photonics 7, 2139 (2020), © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://doi.org/10.1021/acsphotonics.0c00607.
Abstract	Nonlinear optical microscopy can obtain three-dimensionally resolved images within a specimen by exploiting the nonlinear light-matter interaction between the excitation light and sample. However, the image contrast significantly degrades with increasing observation depth because the emitted signal attenuates during light propagation through layers of the tissue before being detected. To obtain high contrast images from deep tissue, we developed saturated excitation (SAX) microscopy using the nonlinearity of near-infrared (NIR) plasmonic scattering from gold nanoshells and gold nanorods. SAX microscopy selectively detects the nonlinear component from the scattering signal generated by nanoparticle probes located at the center of the focal spot. By using this technique, background signals generated at out-of-focal positions are effectively removed. In addition, emitted signals in the NIR from nanoparticle probes efficiently transmit through biological tissue and are ideally suited to image deep parts of the tissue. We experimentally confirmed that scattering intensities from a single gold nanoshell and gold nanorod exhibit nonlinear relations with the excitation intensity of CW laser light at 780 and 1064 nm, respectively. We also demonstrated improvements of image contrast and spatial resolution at the depth of 400 μm in a phantom of muscle tissue by selectively detecting the nonlinear scattering signal component from gold nanoshells.
Publisher	American Chemical Society
Source Title	ACS Photonics
Volume (Issue)	7(8)
Page	2139-2146
Date of Issued	2020-07-01
Language	English
Handle URL	https://hdl.handle.net/11094/103311
eISSN	23304022
Relation.isVersionOf	DOI (Publisher Version) https://doi.org/10.1021/acsphotonics.0c00607
Access Rights	open access
oaire:version	Accepted Manuscript
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCMI Type	Text
DCTERMS.bibliographicCitation	ACS Photonics. 2020, 7(8), p. 2139-2146
DC.title	Nonlinear Scattering of Near-Infrared Light for Imaging Plasmonic Nanoparticles in Deep Tissue
DC.creator	Nishida, Kentaro
	Deka, Gitanjal
	Smith, Nicholas Isaac
	Chu, Shi Wei
	Fujita, Katsumasa
DC.publisher	American Chemical Society
DC.language' scheme='DCTERMS.RFC1766	English
DC.type' scheme='DCTERMS.DCMIType	Text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2020-07-01
DC.identifier	https://doi.org/10.1021/acsphotonics.0c00607
DC.subject	deep tissue imaging
	gold nanoparticle
	near-infrared
	nonlinear
	plasmonics
	super-resolution microscopy
DC.description	This document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS Photonics 7, 2139 (2020), © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://doi.org/10.1021/acsphotonics.0c00607.
DCTERMS.abstract	Nonlinear optical microscopy can obtain three-dimensionally resolved images within a specimen by exploiting the nonlinear light-matter interaction between the excitation light and sample. However, the image contrast significantly degrades with increasing observation depth because the emitted signal attenuates during light propagation through layers of the tissue before being detected. To obtain high contrast images from deep tissue, we developed saturated excitation (SAX) microscopy using the nonlinearity of near-infrared (NIR) plasmonic scattering from gold nanoshells and gold nanorods. SAX microscopy selectively detects the nonlinear component from the scattering signal generated by nanoparticle probes located at the center of the focal spot. By using this technique, background signals generated at out-of-focal positions are effectively removed. In addition, emitted signals in the NIR from nanoparticle probes efficiently transmit through biological tissue and are ideally suited to image deep parts of the tissue. We experimentally confirmed that scattering intensities from a single gold nanoshell and gold nanorod exhibit nonlinear relations with the excitation intensity of CW laser light at 780 and 1064 nm, respectively. We also demonstrated improvements of image contrast and spatial resolution at the depth of 400 μm in a phantom of muscle tissue by selectively detecting the nonlinear scattering signal component from gold nanoshells.
DC.format	103311
citation_title	Nonlinear Scattering of Near-Infrared Light for Imaging Plasmonic Nanoparticles in Deep Tissue
citation_author	Nishida, Kentaro
	Deka, Gitanjal
	Smith, Nicholas Isaac
	Chu, Shi Wei
	Fujita, Katsumasa
citation_publisher	American Chemical Society
citation_language	English
citation_date	2020-07-01
citation_journal_title	ACS Photonics
citation_volume	7
citation_issue	8
citation_firstpage	2139
citation_lastpage	2146
citation_doi	https://doi.org/10.1021/acsphotonics.0c00607
citation_public_url	https://hdl.handle.net/11094/103311

Nonlinear Scattering of Near-Infrared Light for Imaging Plasmonic Nanoparticles in Deep Tissue

Link to primary information

Item Information

Show details