Using redox-sensitive mitochondrial cytochrome Raman bands for label-free detection of mitochondrial dysfunction

Morimoto, Takeshi; Chiu, Liang Da; Kanda, Hiroyuki; Kawagoe, Hiroyuki; Ozawa, Takeaki; Nakamura, Makoto; Nishida, Kohji; Fujita, Katsumasa; Fujikado, Takashi

doi:https://doi.org/10.1039/c8an02213e

Using redox-sensitive mitochondrial cytochrome Raman bands for label-free detection of mitochondrial dysfunction

Morimoto, Takeshi; Chiu, Liang Da; Kanda, Hiroyuki et al.

Analyst, 2019, 144(8), 2531-2540

Number of Access:327（2026-02-18 11:34 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/103313

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Title	Using redox-sensitive mitochondrial cytochrome Raman bands for label-free detection of mitochondrial dysfunction
Creator	NRID 1000000530198 1000000530198 ORCID 0000-0002-9653-1556 0000-0002-9653-1556 researchmap permalink tmorimoto-eye tmorimoto-eye Scopus Author ID 24503733900 24503733900 CreatorName Morimoto, Takeshi
	NRID 1000080648220 1000080648220 CreatorName Chiu, Liang Da
	NRID 1000050570248 1000050570248 CreatorName Kanda, Hiroyuki
	CreatorName Kawagoe, Hiroyuki
	NRID 1000040302806 1000040302806 CreatorName Ozawa, Takeaki
	NRID 1000080273788 1000080273788 CreatorName Nakamura, Makoto
	NRID 1000040244610 1000040244610 ORCID 0000-0001-9069-3610 0000-0001-9069-3610 researchmap permalink read0131487 read0131487 Scopus Author ID 57211973404 57211973404 CreatorName Nishida, Kohji
	NRID 1000080362664 1000080362664 ORCID 0000-0002-2284-375X 0000-0002-2284-375X researchmap permalink read0089911 read0089911 Scopus Author ID 7404058829 7404058829 CreatorName Fujita, Katsumasa
	NRID 1000050243233 1000050243233 researchmap permalink fuji4313 fuji4313 CreatorName Fujikado, Takashi
Description	Reproduced from Analyst 144, 2531 (2019) with permission from the Royal Society of Chemistry.
Abstract	Mitochondrial activity is a widely used criterion to judge the metabolic condition of a living specimen. Numerous methods have been developed for related analyses, including the detection of O₂ consumption, trans-membrane potential, and ATP production. In this study, we demonstrate that the redox state of cytochromes can serve as a sensitive mitochondrial activity indicator in glutamate-stressed neuronal cells. Mitochondrial dysfunction was detected by Raman imaging as early as 30 min after glutamate-stress induction. By comparing this result with other commonly used mitochondrial function assays, we found Raman imaging has a similar sensitivity to ATP production and trans-membrane potential assays. Other viability tests, such as MTT assay and ROS production tests, showed a slower response than our method. A thorough understanding of cytochrome dynamics with our new method will help establish Raman spectroscopy as a competitive clinical diagnosis tool for neurodegenerative diseases involving mitochondrial dysfunction.
Publisher	Royal Society of Chemistry
Source Title	Analyst
Volume (Issue)	144(8)
Page	2531-2540
Date of Issued	2019-02-15
Language	English
Handle URL	https://hdl.handle.net/11094/103313
PISSN	00032654
eISSN	13645528
Relation.isVersionOf	DOI (Publisher Version) https://doi.org/10.1039/c8an02213e
Access Rights	open access
oaire:version	Accepted Manuscript
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCMI Type	Text
DCTERMS.bibliographicCitation	Analyst. 2019, 144(8), p. 2531-2540
DC.title	Using redox-sensitive mitochondrial cytochrome Raman bands for label-free detection of mitochondrial dysfunction
DC.creator	Morimoto, Takeshi
	Chiu, Liang Da
	Kanda, Hiroyuki
	Kawagoe, Hiroyuki
	Ozawa, Takeaki
	Nakamura, Makoto
	Nishida, Kohji
	Fujita, Katsumasa
	Fujikado, Takashi
DC.publisher	Royal Society of Chemistry
DC.language' scheme='DCTERMS.RFC1766	English
DC.type' scheme='DCTERMS.DCMIType	Text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2019-02-15
DC.identifier	https://doi.org/10.1039/c8an02213e
DC.description	Reproduced from Analyst 144, 2531 (2019) with permission from the Royal Society of Chemistry.
DCTERMS.abstract	Mitochondrial activity is a widely used criterion to judge the metabolic condition of a living specimen. Numerous methods have been developed for related analyses, including the detection of O₂ consumption, trans-membrane potential, and ATP production. In this study, we demonstrate that the redox state of cytochromes can serve as a sensitive mitochondrial activity indicator in glutamate-stressed neuronal cells. Mitochondrial dysfunction was detected by Raman imaging as early as 30 min after glutamate-stress induction. By comparing this result with other commonly used mitochondrial function assays, we found Raman imaging has a similar sensitivity to ATP production and trans-membrane potential assays. Other viability tests, such as MTT assay and ROS production tests, showed a slower response than our method. A thorough understanding of cytochrome dynamics with our new method will help establish Raman spectroscopy as a competitive clinical diagnosis tool for neurodegenerative diseases involving mitochondrial dysfunction.
DC.format	103313
citation_title	Using redox-sensitive mitochondrial cytochrome Raman bands for label-free detection of mitochondrial dysfunction
citation_author	Morimoto, Takeshi
	Chiu, Liang Da
	Kanda, Hiroyuki
	Kawagoe, Hiroyuki
	Ozawa, Takeaki
	Nakamura, Makoto
	Nishida, Kohji
	Fujita, Katsumasa
	Fujikado, Takashi
citation_publisher	Royal Society of Chemistry
citation_language	English
citation_date	2019-02-15
citation_journal_title	Analyst
citation_volume	144
citation_issue	8
citation_firstpage	2531
citation_lastpage	2540
citation_doi	https://doi.org/10.1039/c8an02213e
citation_public_url	https://hdl.handle.net/11094/103313

Using redox-sensitive mitochondrial cytochrome Raman bands for label-free detection of mitochondrial dysfunction

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