Spectral focusing in picosecond pulsed stimulated Raman scattering microscopy

Koike, Kota; Smith, Nicholas I.; Fujita, Katsumasa

Biomedical Optics Express, 2022, 13(2), 995-1004

Number of Access:115（2026-02-18 16:18 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/103329

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Title
Spectral focusing in picosecond pulsed stimulated Raman scattering microscopy
Creator
CreatorName
Koike, Kota
NRID
1000010362592 1000010362592
ORCID
0000-0002-7835-199X 0000-0002-7835-199X
researchmap permalink
read0089935 read0089935
Scopus Author ID
55262815400 55262815400
CreatorName
Smith, Nicholas I.
NRID
1000080362664 1000080362664
ORCID
0000-0002-2284-375X 0000-0002-2284-375X
researchmap permalink
read0089911 read0089911
Scopus Author ID
7404058829 7404058829
CreatorName
Fujita, Katsumasa
Description
Koike K., Smith N.I., Fujita K.. Spectral focusing in picosecond pulsed stimulated Raman scattering microscopy. Biomedical Optics Express 13, 995 (2022); https://doi.org/10.1364/BOE.445640.
Abstract
We introduce spectral focusing of picosecond laser pulses in stimulated Raman scattering (SRS) microscopy to improve spectral resolution, reduce nonlinear background signals, and decrease nonlinear photodamage. We produce a pair of 14 ps pump and Stokes laser pulses by spectral focusing of a 2 ps laser and achieve a spectral resolution of 2 cm⁻¹. Due to instantaneous narrow-band excitation, we find that the chirped 14 ps laser pulses can be used to improve the signal-to-background ratio in SRS microscopy of various samples such as polymer particles and small molecules in HeLa cells. The lower peak powers produced by chirped picosecond laser pulses also reduce nonlinear photodamage, allowing long-term SRS imaging of living cells with higher SNR.
Publisher
The Optical Society
Source Title
Biomedical Optics Express
Volume (Issue)
13(2)
Page
995-1004
Date of Issued
2022-01-27
Language
English
Handle URL
https://hdl.handle.net/11094/103329
eISSN
21567085
Relation.isIdenticalTo
DOI (Publisher Version)
https://doi.org/10.1364/BOE.445640
Access Rights
open access
CopyRight
© 2022 Optica Publishing Group. Users may use, reuse, and build upon the article, or use the article for text or data mining, so long as such uses are for non-commercial purposes and appropriate attribution is maintained. All other rights are reserved.
oaire:version
Version of Record
Category
学術雑誌論文 Journal Article