Vacuum ultraviolet-induced degradation of polyethylene and polyvinyl chloride micro/nanoplastics enhances their cytotoxicity and lipid peroxidation level

Manabe, Sota; Haga, Yuya; Tsujino, Hirofumi; Ikuno, Yudai; Idehara, Wakaba; Hokaku, Mii; Bo Bo Aung, Phyo; Asahara, Haruyasu; Higashisaka, Kazuma; Tsutsumi, Yasuo

doi:https://doi.org/10.1016/j.ecoenv.2025.119030

Vacuum ultraviolet-induced degradation of polyethylene and polyvinyl chloride micro/nanoplastics enhances their cytotoxicity and lipid peroxidation level

Manabe, Sota; Haga, Yuya; Tsujino, Hirofumi et al.

Ecotoxicology and Environmental Safety, 2025, 303, 119030

Number of Access:5（2026-01-03 12:04 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/103498

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Title	Vacuum ultraviolet-induced degradation of polyethylene and polyvinyl chloride micro/nanoplastics enhances their cytotoxicity and lipid peroxidation level
Creator	CreatorName Manabe, Sota
	NRID 1000030888863 1000030888863 ORCID 0000-0003-4148-2256 0000-0003-4148-2256 researchmap permalink 30888863 30888863 Scopus Author ID 57211023415 57211023415 CreatorName Haga, Yuya
	NRID 1000010707144 1000010707144 researchmap permalink htsujino htsujino Scopus Author ID 36667726200 36667726200 CreatorName Tsujino, Hirofumi
	CreatorName Ikuno, Yudai
	CreatorName Idehara, Wakaba
	CreatorName Hokaku, Mii
	CreatorName Bo Bo Aung, Phyo
	NRID 1000020632318 1000020632318 ORCID 0000-0002-1808-7373 0000-0002-1808-7373 researchmap permalink pikurin3 pikurin3 Scopus Author ID 8856988200 8856988200 CreatorName Asahara, Haruyasu
	NRID 1000020646757 1000020646757 researchmap permalink read646757 read646757 Scopus Author ID 35603386200 35603386200 CreatorName Higashisaka, Kazuma
	NRID 1000050263306 1000050263306 researchmap permalink ytsutsumi ytsutsumi Scopus Author ID 7202474093 7202474093 CreatorName Tsutsumi, Yasuo
Subject	Cell death mechanism
	Macrophage
	Reactive oxygen species
	Surface degradation
	Toxicity assessment
Description	Manabe S., Haga Y., Tsujino H., et al. Vacuum ultraviolet-induced degradation of polyethylene and polyvinyl chloride micro/nanoplastics enhances their cytotoxicity and lipid peroxidation level. Ecotoxicology and Environmental Safety 303, 119030 (2025); https://doi.org/10.1016/j.ecoenv.2025.119030.
Abstract	Plastic particles smaller than 5 mm, termed microplastics, have raised concerns regarding their potential impact on human health. Even smaller particles (<1 µm), classified as nanoplastics, also warrant further investigation. Since these plastic particles have been detected in various human tissues, their biological effects must be thoroughly evaluated. These plastics originate from diverse polymer types and exhibit complex physicochemical properties such as size, shape, and surface degradation in the environment. However, current studies mainly utilize polystyrene beads, which may not exist widely in the environment. Therefore, the use of environmentally relevant micro- and nanoplastics for safety assessments is crucial. Focusing mainly on surface degradation, this study aimed to elucidate detailed mechanisms of cell death caused by micro- and nanoplastics and assess their universality across different polymer types, including polyethylene (PE) and polyvinyl chloride (PVC). Surface-degraded samples of PE and PVC with different particle sizes, including microplastics and nanoplastics, were prepared and their cell death mechanisms were evaluated in murine macrophage RAW264.7 cells. Transcriptomic analysis revealed that degraded PE microplastics upregulated ferroptosis-related gene expressions and increased reactive oxygen species level and lipid peroxidation. Cell death and lipid peroxidation induction were also examined in other polymer types, including PVC microplastics as well as PE and PVC nanoplastics. Notably, only the degraded samples induced cell death and lipid peroxidation for all tested particles. Given the role of lipid peroxidation in various diseases such as neurological dysfunction and ischemia-reperfusion injury, our findings highlight the potential health risks of environmental micro- and nanoplastics.
Publisher	Academic Press
Source Title	Ecotoxicology and Environmental Safety
Volume (Issue)	303
Page	119030
Date of Issued	2025-09-10
Language	English
Handle URL	https://hdl.handle.net/11094/103498
PISSN	01476513
eISSN	10902414
Relation.isIdenticalTo	DOI (Publisher Version) https://doi.org/10.1016/j.ecoenv.2025.119030
Access Rights	open access
CopyRight	This article is licensed under a Creative Commons Attribution 4.0 International License.
oaire:version	Version of Record
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCMI Type	Text
DCTERMS.bibliographicCitation	Ecotoxicology and Environmental Safety. 2025, 303, p. 119030
DC.title	Vacuum ultraviolet-induced degradation of polyethylene and polyvinyl chloride micro/nanoplastics enhances their cytotoxicity and lipid peroxidation level
DC.creator	Manabe, Sota
	Haga, Yuya
	Tsujino, Hirofumi
	Ikuno, Yudai
	Idehara, Wakaba
	Hokaku, Mii
	Bo Bo Aung, Phyo
	Asahara, Haruyasu
	Higashisaka, Kazuma
	Tsutsumi, Yasuo
DC.publisher	Academic Press
DC.language' scheme='DCTERMS.RFC1766	English
DC.type' scheme='DCTERMS.DCMIType	Text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2025-09-10
DC.identifier	https://doi.org/10.1016/j.ecoenv.2025.119030
DC.subject	Cell death mechanism
	Macrophage
	Reactive oxygen species
	Surface degradation
	Toxicity assessment
DC.description	Manabe S., Haga Y., Tsujino H., et al. Vacuum ultraviolet-induced degradation of polyethylene and polyvinyl chloride micro/nanoplastics enhances their cytotoxicity and lipid peroxidation level. Ecotoxicology and Environmental Safety 303, 119030 (2025); https://doi.org/10.1016/j.ecoenv.2025.119030.
DCTERMS.abstract	Plastic particles smaller than 5 mm, termed microplastics, have raised concerns regarding their potential impact on human health. Even smaller particles (<1 µm), classified as nanoplastics, also warrant further investigation. Since these plastic particles have been detected in various human tissues, their biological effects must be thoroughly evaluated. These plastics originate from diverse polymer types and exhibit complex physicochemical properties such as size, shape, and surface degradation in the environment. However, current studies mainly utilize polystyrene beads, which may not exist widely in the environment. Therefore, the use of environmentally relevant micro- and nanoplastics for safety assessments is crucial. Focusing mainly on surface degradation, this study aimed to elucidate detailed mechanisms of cell death caused by micro- and nanoplastics and assess their universality across different polymer types, including polyethylene (PE) and polyvinyl chloride (PVC). Surface-degraded samples of PE and PVC with different particle sizes, including microplastics and nanoplastics, were prepared and their cell death mechanisms were evaluated in murine macrophage RAW264.7 cells. Transcriptomic analysis revealed that degraded PE microplastics upregulated ferroptosis-related gene expressions and increased reactive oxygen species level and lipid peroxidation. Cell death and lipid peroxidation induction were also examined in other polymer types, including PVC microplastics as well as PE and PVC nanoplastics. Notably, only the degraded samples induced cell death and lipid peroxidation for all tested particles. Given the role of lipid peroxidation in various diseases such as neurological dysfunction and ischemia-reperfusion injury, our findings highlight the potential health risks of environmental micro- and nanoplastics.
DC.rights	This article is licensed under a Creative Commons Attribution 4.0 International License.
DC.format	103498
citation_title	Vacuum ultraviolet-induced degradation of polyethylene and polyvinyl chloride micro/nanoplastics enhances their cytotoxicity and lipid peroxidation level
citation_author	Manabe, Sota
	Haga, Yuya
	Tsujino, Hirofumi
	Ikuno, Yudai
	Idehara, Wakaba
	Hokaku, Mii
	Bo Bo Aung, Phyo
	Asahara, Haruyasu
	Higashisaka, Kazuma
	Tsutsumi, Yasuo
citation_publisher	Academic Press
citation_language	English
citation_date	2025-09-10
citation_journal_title	Ecotoxicology and Environmental Safety
citation_volume	303
citation_firstpage	119030
citation_doi	https://doi.org/10.1016/j.ecoenv.2025.119030
citation_public_url	https://hdl.handle.net/11094/103498

Vacuum ultraviolet-induced degradation of polyethylene and polyvinyl chloride micro/nanoplastics enhances their cytotoxicity and lipid peroxidation level

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