Silica nanoparticles reduce fetal weight in mice and induce an inflammatory response in human extravillous trophoblast cells

Yamamoto, Rena; Higashisaka, Kazuma; Sakai, Risa; Nakamoto, Yurina; Serizawa, Momoe; Haga, Yuya; Tsutsumi, Yasuo

doi:https://doi.org/10.1016/j.bbrc.2025.152803

Silica nanoparticles reduce fetal weight in mice and induce an inflammatory response in human extravillous trophoblast cells

Yamamoto, Rena; Higashisaka, Kazuma; Sakai, Risa et al.

Biochemical and Biophysical Research Communications, 2025, 788, 152803

Number of Access:645（2026-10-12 13:49 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/103512

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Title	Silica nanoparticles reduce fetal weight in mice and induce an inflammatory response in human extravillous trophoblast cells
Creator	CreatorName Yamamoto, Rena
	NRID 1000020646757 1000020646757 researchmap permalink read646757 read646757 Scopus Author ID 35603386200 35603386200 CreatorName Higashisaka, Kazuma
	CreatorName Sakai, Risa
	CreatorName Nakamoto, Yurina
	CreatorName Serizawa, Momoe
	NRID 1000030888863 1000030888863 ORCID 0000-0003-4148-2256 0000-0003-4148-2256 researchmap permalink 30888863 30888863 Scopus Author ID 57211023415 57211023415 CreatorName Haga, Yuya
	NRID 1000050263306 1000050263306 researchmap permalink ytsutsumi ytsutsumi Scopus Author ID 7202474093 7202474093 CreatorName Tsutsumi, Yasuo
Subject	Extravillous trophoblast cells
	Fetal toxicity
	Inflammation
	Placental toxicity
	Silica nanoparticle
Description	Yamamoto R., Higashisaka K., Sakai R., et al. Silica nanoparticles reduce fetal weight in mice and induce an inflammatory response in human extravillous trophoblast cells. Biochemical and Biophysical Research Communications 788, 152803 (2025); https://doi.org/10.1016/j.bbrc.2025.152803.
Abstract	Advances in nanotechnology make the exposure to nanoparticles inevitable, potentially resulting in unexpected biological effects, particularly in pregnant women and fetuses. In this study, we used silica nanoparticles with a size of 10 nm (nSP10) and evaluated their toxicity during pregnancy in BALB/c mice. To evaluate the effect of nSP10 on the remodeling of uterine spiral arteries by extravillous trophoblast (EVT) cells, we used the human placental cell line HTR-8/SVneo, a model for EVT cells. On gestational day (GD) 10, mice were intravenously injected with a single dose of nSP10. On GD17, placental weight was not changed significantly, but fetal weight was significantly decreased by the treatment. In wound healing assay, nSP10 treatment significantly decreased the relative migration area of HTR-8/SVneo cells in a concentration-dependent manner, suggesting that nSP10 inhibits cell migration. In the cytokine array assay, nSP10-treated cells tended to produce more IL-6 and IL-8 than non-treated cells. In ELISA, nSP10 significantly increased IL-6 and IL-8 production, and JSH-23 (an inhibitor of NF-κB nuclear translocation) significantly suppressed this effect, suggesting that nSP10 increases IL-6 and IL-8 production by inducing nuclear translocation of NF-κB. Our data suggest that nSP10 reduces mouse fetal weight and may inhibit migration and induce inflammatory response of human extravillous trophoblast cells.
Publisher	Elsevier B.V.
Source Title	Biochemical and Biophysical Research Communications
Volume (Issue)	788
Page	152803
Date of Issued	2025-10-16
Language	English
Handle URL	https://hdl.handle.net/11094/103512
PISSN	0006291X
eISSN	10902104
Relation.isIdenticalTo	DOI (Publisher Version) https://doi.org/10.1016/j.bbrc.2025.152803
Access Rights	open access
CopyRight	This article is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
oaire:version	Version of Record
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCMI Type	Text
DCTERMS.bibliographicCitation	Biochemical and Biophysical Research Communications. 2025, 788, p. 152803
DC.title	Silica nanoparticles reduce fetal weight in mice and induce an inflammatory response in human extravillous trophoblast cells
DC.creator	Yamamoto, Rena
	Higashisaka, Kazuma
	Sakai, Risa
	Nakamoto, Yurina
	Serizawa, Momoe
	Haga, Yuya
	Tsutsumi, Yasuo
DC.publisher	Elsevier B.V.
DC.language' scheme='DCTERMS.RFC1766	English
DC.type' scheme='DCTERMS.DCMIType	Text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2025-10-16
DC.identifier	https://doi.org/10.1016/j.bbrc.2025.152803
DC.subject	Extravillous trophoblast cells
	Fetal toxicity
	Inflammation
	Placental toxicity
	Silica nanoparticle
DC.description	Yamamoto R., Higashisaka K., Sakai R., et al. Silica nanoparticles reduce fetal weight in mice and induce an inflammatory response in human extravillous trophoblast cells. Biochemical and Biophysical Research Communications 788, 152803 (2025); https://doi.org/10.1016/j.bbrc.2025.152803.
DCTERMS.abstract	Advances in nanotechnology make the exposure to nanoparticles inevitable, potentially resulting in unexpected biological effects, particularly in pregnant women and fetuses. In this study, we used silica nanoparticles with a size of 10 nm (nSP10) and evaluated their toxicity during pregnancy in BALB/c mice. To evaluate the effect of nSP10 on the remodeling of uterine spiral arteries by extravillous trophoblast (EVT) cells, we used the human placental cell line HTR-8/SVneo, a model for EVT cells. On gestational day (GD) 10, mice were intravenously injected with a single dose of nSP10. On GD17, placental weight was not changed significantly, but fetal weight was significantly decreased by the treatment. In wound healing assay, nSP10 treatment significantly decreased the relative migration area of HTR-8/SVneo cells in a concentration-dependent manner, suggesting that nSP10 inhibits cell migration. In the cytokine array assay, nSP10-treated cells tended to produce more IL-6 and IL-8 than non-treated cells. In ELISA, nSP10 significantly increased IL-6 and IL-8 production, and JSH-23 (an inhibitor of NF-κB nuclear translocation) significantly suppressed this effect, suggesting that nSP10 increases IL-6 and IL-8 production by inducing nuclear translocation of NF-κB. Our data suggest that nSP10 reduces mouse fetal weight and may inhibit migration and induce inflammatory response of human extravillous trophoblast cells.
DC.rights	This article is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
DC.format	103512
citation_title	Silica nanoparticles reduce fetal weight in mice and induce an inflammatory response in human extravillous trophoblast cells
citation_author	Yamamoto, Rena
	Higashisaka, Kazuma
	Sakai, Risa
	Nakamoto, Yurina
	Serizawa, Momoe
	Haga, Yuya
	Tsutsumi, Yasuo
citation_publisher	Elsevier B.V.
citation_language	English
citation_date	2025-10-16
citation_journal_title	Biochemical and Biophysical Research Communications
citation_volume	788
citation_firstpage	152803
citation_doi	https://doi.org/10.1016/j.bbrc.2025.152803
citation_public_url	https://hdl.handle.net/11094/103512

Silica nanoparticles reduce fetal weight in mice and induce an inflammatory response in human extravillous trophoblast cells

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