Suppression of mechanical hypersensitivity and change in the expression of the dopamine D2 receptor by administration of anti-CGRP antibody into the trigeminal ganglion in trigeminal neuropathic pain model rats

Maegawa, Hiroharu; Usami, Nayuka; Kudo, Chiho et al.

PLOS One, 2025, 20(5), e0323810

Number of Access:17（2026-02-18 05:32 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/103683

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Title
Suppression of mechanical hypersensitivity and change in the expression of the dopamine D2 receptor by administration of anti-CGRP antibody into the trigeminal ganglion in trigeminal neuropathic pain model rats
Creator
NRID
1000010711012 1000010711012
ORCID
0000-0001-8331-546X 0000-0001-8331-546X
researchmap permalink
maega maega
Scopus Author ID
36604752300 36604752300
CreatorName
Maegawa, Hiroharu
CreatorName
Usami, Nayuka
NRID
1000020533110 1000020533110
ORCID
0000-0002-1689-3566 0000-0002-1689-3566
researchmap permalink
chihokudo chihokudo
Scopus Author ID
6701693347 6701693347
CreatorName
Kudo, Chiho
NRID
1000030218250 1000030218250
researchmap permalink
19580710 19580710
Scopus Author ID
24351577000 24351577000
CreatorName
Niwa, Hitoshi
Description
Maegawa H., Usami N., Kudo C., et al. (2025) Suppression of mechanical hypersensitivity and change in the expression of the dopamine D2 receptor by administration of anti-CGRP antibody into the trigeminal ganglion in trigeminal neuropathic pain model rats. PLOS One 20, e0323810. https://doi.org/10.1371/journal.pone.0323810.
Abstract
Calcitonin gene-related peptide (CGRP) and dopamine D2 receptor (D2 receptor) are associated with neuropathic pain. However, their relationship is not well understood. To better establish their relationship in trigeminal neuropathic pain, we examined male rats with infraorbital nerve (ION) ligation. Rats with ION ligation were administered CGRP or anti-CGRP antibody into the trigeminal ganglion (TG). The change in the head-withdrawal threshold was measured using von Frey filament. Immunohistochemical staining for phosphorylated extracellular signal-regulated kinase (pERK) was also performed in the trigeminal spinal subnucleus caudalis (Vc). CGRP was detected in the TG and Vc by immunohistochemical staining, and glial fibrillary acidic protein (GFAP) in the TG and dopamine D2 receptor in the Vc were detected in the same way. Antibody administration restored the head-withdrawal threshold to mechanical stimuli, which had decreased after ION ligation. Furthermore, the number of pERK-immunoreactive (-IR) neurons in the Vc, which increased following ION ligation, declined. The ratio of CGRP-IR TG neurons, large-sized CGRP-IR TG neurons, and TG neurons encircled with the GFAP-IR cells increased after ION ligation and decreased after antibody administration. Moreover, the immunoreactivity of CGRP and D2 receptor in the Vc increased after ION ligation and decreased after antibody administration. There were no significant differences in the head-withdrawal threshold, pERK-IR cell count, ratio of CGRP-IR TG neurons, ratio of size of CGRP-IR TG neurons, ratio of TG neurons encircled with GFAP-IR cells, and immunoreactivity of CGRP and D2 receptor in the Vc between the ION-ligated rats with and without CGRP. These findings suggest that the administration of an anti-CGRP antibody into the TG is involved in the suppression of trigeminal neuropathic pain and the D2 receptor expression in the Vc.
Publisher
Public Library of Science
Source Title
PLOS One
Volume (Issue)
20(5)
Page
e0323810
Date of Issued
2025-05-14
Language
English
Handle URL
https://hdl.handle.net/11094/103683
eISSN
19326203
Relation.isIdenticalTo
DOI (Publisher Version)
https://doi.org/10.1371/journal.pone.0323810
Access Rights
open access
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This article is licensed under a Creative Commons Attribution 4.0 International License.
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Category
学術雑誌論文 Journal Article