Distribution of Helicobacter pylori in the Oral Cavity of Patients With Tooth Extractions at Various Locations and Ages

Hamada, Masakazu; Nomura, Ryota; Ogaya, Yuko; Nishiyama, Kyoko; Kadota, Tamami; Nakano, Kazuhiko

doi:https://doi.org/10.1016/j.identj.2025.103977

Distribution of Helicobacter pylori in the Oral Cavity of Patients With Tooth Extractions at Various Locations and Ages

Hamada, Masakazu; Nomura, Ryota; Ogaya, Yuko et al.

International Dental Journal, 2025, 76, 103977

Number of Access:20（2026-02-05 17:48 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/103684

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Title	Distribution of Helicobacter pylori in the Oral Cavity of Patients With Tooth Extractions at Various Locations and Ages
Creator	NRID 1000080506361 1000080506361 researchmap permalink 6361 6361 Scopus Author ID 36616148000 36616148000 CreatorName Hamada, Masakazu
	NRID 1000090437385 1000090437385 CreatorName Nomura, Ryota
	NRID 1000040803078 1000040803078 researchmap permalink y_ogaya y_ogaya Scopus Author ID 56460237100 56460237100 CreatorName Ogaya, Yuko
	NRID 1000070846542 1000070846542 researchmap permalink 02205 02205 CreatorName Nishiyama, Kyoko
	NRID 1000010908643 1000010908643 researchmap permalink kadota-tamami kadota-tamami CreatorName Kadota, Tamami
	NRID 1000000379083 1000000379083 researchmap permalink read0202427 read0202427 Scopus Author ID 7402011667 7402011667 CreatorName Nakano, Kazuhiko
Subject	Extracted tooth
	Helicobacter pylori
	Molecular biological analysis
	Oral specimens
Description	Hamada M., Nomura R., Ogaya Y., et al. Distribution of Helicobacter pylori in the Oral Cavity of Patients With Tooth Extractions at Various Locations and Ages. International Dental Journal 76, 103977 (2026); https://doi.org/https://doi.org/10.1016/j.identj.2025.103977.
Abstract	Background Helicobacter pylori is well known to cause gastric cancer, gastric ulcers and duodenal ulcers. The oral cavity has been implicated as a reservoir of H. pylori in adulthood. However, there are currently no detailed studies focused on the tooth type colonised by H. pylori and/or the age of subjects. Therefore, we examined the distribution of H. pylori in oral specimens collected from 163 Japanese adults who underwent tooth extraction. Materials and Methods Bacterial DNA was extracted from 163 Japanese adults, and H. pylori was detected using PCR. Surface deposits on the occlusal surface were observed using an electron microscope. Results A PCR analysis revealed H. pylori in 17 saliva samples (10.4%), 27 tooth samples (16.6%) and 19 pulp samples (11.7%). We also confirmed surface deposits on the occlusal surface of deeply impacted and obviously stained third molars using electron microscopic images, and H. pylori was detected in 11 out of 33 (33.3%) extracted impacted third molars. We then categorised tooth extraction sites into 3 patterns: teeth other than the third molars, erupted third molars and impacted third molars, and compared the ages of subjects who tested positive and negative for H. pylori . Among impacted third molars, H. pylori -positive subjects were significantly older than H. pylori -negative subjects for all oral samples tested ( P < .05). We then examined these subjects based on the localisation of H. pylori in saliva, teeth and dental pulp, and found that subjects with teeth that were positive for H. pylori were significantly older than those with teeth that were negative for H. pylori ( P < .05). Conclusions H. pylori may colonise not only erupted teeth, but also impacted teeth, particularly in the elderly. Impacted third molars left for a long period may be a reservoir for H. pylori .
Publisher	Elsevier Inc.
Source Title	International Dental Journal
Volume (Issue)	76
Page	103977
Date of Issued	2025-10-29
Language	English
Handle URL	https://hdl.handle.net/11094/103684
PISSN	00206539
eISSN	1875595X
Relation.isIdenticalTo	DOI (Publisher Version) https://doi.org/10.1016/j.identj.2025.103977
Access Rights	open access
CopyRight	This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
oaire:version	Version of Record
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCMI Type	Text
DCTERMS.bibliographicCitation	International Dental Journal. 2025, 76, p. 103977
DC.title	Distribution of Helicobacter pylori in the Oral Cavity of Patients With Tooth Extractions at Various Locations and Ages
DC.creator	Hamada, Masakazu
	Nomura, Ryota
	Ogaya, Yuko
	Nishiyama, Kyoko
	Kadota, Tamami
	Nakano, Kazuhiko
DC.publisher	Elsevier Inc.
DC.language' scheme='DCTERMS.RFC1766	English
DC.type' scheme='DCTERMS.DCMIType	Text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2025-10-29
DC.identifier	https://doi.org/10.1016/j.identj.2025.103977
DC.subject	Extracted tooth
	Helicobacter pylori
	Molecular biological analysis
	Oral specimens
DC.description	Hamada M., Nomura R., Ogaya Y., et al. Distribution of Helicobacter pylori in the Oral Cavity of Patients With Tooth Extractions at Various Locations and Ages. International Dental Journal 76, 103977 (2026); https://doi.org/https://doi.org/10.1016/j.identj.2025.103977.
DCTERMS.abstract	Background Helicobacter pylori is well known to cause gastric cancer, gastric ulcers and duodenal ulcers. The oral cavity has been implicated as a reservoir of H. pylori in adulthood. However, there are currently no detailed studies focused on the tooth type colonised by H. pylori and/or the age of subjects. Therefore, we examined the distribution of H. pylori in oral specimens collected from 163 Japanese adults who underwent tooth extraction. Materials and Methods Bacterial DNA was extracted from 163 Japanese adults, and H. pylori was detected using PCR. Surface deposits on the occlusal surface were observed using an electron microscope. Results A PCR analysis revealed H. pylori in 17 saliva samples (10.4%), 27 tooth samples (16.6%) and 19 pulp samples (11.7%). We also confirmed surface deposits on the occlusal surface of deeply impacted and obviously stained third molars using electron microscopic images, and H. pylori was detected in 11 out of 33 (33.3%) extracted impacted third molars. We then categorised tooth extraction sites into 3 patterns: teeth other than the third molars, erupted third molars and impacted third molars, and compared the ages of subjects who tested positive and negative for H. pylori . Among impacted third molars, H. pylori -positive subjects were significantly older than H. pylori -negative subjects for all oral samples tested ( P < .05). We then examined these subjects based on the localisation of H. pylori in saliva, teeth and dental pulp, and found that subjects with teeth that were positive for H. pylori were significantly older than those with teeth that were negative for H. pylori ( P < .05). Conclusions H. pylori may colonise not only erupted teeth, but also impacted teeth, particularly in the elderly. Impacted third molars left for a long period may be a reservoir for H. pylori .
DC.rights	This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
DC.format	103684
citation_title	Distribution of Helicobacter pylori in the Oral Cavity of Patients With Tooth Extractions at Various Locations and Ages
citation_author	Hamada, Masakazu
	Nomura, Ryota
	Ogaya, Yuko
	Nishiyama, Kyoko
	Kadota, Tamami
	Nakano, Kazuhiko
citation_publisher	Elsevier Inc.
citation_language	English
citation_date	2025-10-29
citation_journal_title	International Dental Journal
citation_volume	76
citation_firstpage	103977
citation_doi	https://doi.org/10.1016/j.identj.2025.103977
citation_public_url	https://hdl.handle.net/11094/103684

Distribution of Helicobacter pylori in the Oral Cavity of Patients With Tooth Extractions at Various Locations and Ages

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