Rapid and Accurate Diagnosis of Breast Cancer by Fine-Needle Aspiration Biopsy Using the “Click-to-Sense” Method

Kitahara, Yuri; Tanei, Tomonori; Hatano, Takaaki; Pradipta, Ambara R.; Morimoto, Koji; Nagatomo, Tadasuke; Abe, Kaori; Masunaga, Nanae; Mishima, Chieko; Yoshinami, Tetsuhiro; Tsukabe, Masami; Sota, Yoshiaki; Miyake, Tomohiro; Shimoda, Masafumi; Motoyama, Yuichi; Morii, Eiichi; Tanaka, Katsunori; Shimazu, Kenzo

doi:https://doi.org/10.1002/cam4.71525

Rapid and Accurate Diagnosis of Breast Cancer by Fine-Needle Aspiration Biopsy Using the “Click-to-Sense” Method

Kitahara, Yuri; Tanei, Tomonori; Hatano, Takaaki et al.

Cancer Medicine, 2026, 15(2), e71525

Number of Access:39（2026-04-06 20:45 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/104243

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Title	Rapid and Accurate Diagnosis of Breast Cancer by Fine-Needle Aspiration Biopsy Using the “Click-to-Sense” Method
Creator	CreatorName Kitahara, Yuri
	NRID 1000080771518 1000080771518 ORCID 0000-0003-2183-8681 0000-0003-2183-8681 researchmap permalink tanei tanei Scopus Author ID 6506192669 6506192669 CreatorName Tanei, Tomonori
	CreatorName Hatano, Takaaki
	NRID 1000090631648 1000090631648 CreatorName Pradipta, Ambara R.
	NRID 1000000599996 1000000599996 CreatorName Morimoto, Koji
	CreatorName Nagatomo, Tadasuke
	NRID 1000080587593 1000080587593 CreatorName Abe, Kaori
	NRID 1000060961620 1000060961620 ORCID 0000-0002-6164-5107 0000-0002-6164-5107 researchmap permalink nnnnnanae nnnnnanae Scopus Author ID 57195636812 57195636812 CreatorName Masunaga, Nanae
	NRID 1000031009103 1000031009103 ORCID 0009-0009-9610-5758 0009-0009-9610-5758 researchmap permalink chiekomishima chiekomishima Scopus Author ID 56692673200 56692673200 CreatorName Mishima, Chieko
	NRID 1000030894240 1000030894240 researchmap permalink osaka-yoshi osaka-yoshi Scopus Author ID 36009656500 36009656500 CreatorName Yoshinami, Tetsuhiro
	ORCID 0009-0009-3284-0833 0009-0009-3284-0833 researchmap permalink masami-tsukabe masami-tsukabe Scopus Author ID 55908971300 55908971300 CreatorName Tsukabe, Masami
	NRID 1000080943779 1000080943779 ORCID 0000-0001-5963-652X 0000-0001-5963-652X researchmap permalink y_sota123 y_sota123 Scopus Author ID 56308090600 56308090600 CreatorName Sota, Yoshiaki
	NRID 1000000636110 1000000636110 ORCID 0000-0002-9362-9822 0000-0002-9362-9822 researchmap permalink tomohiromiyake tomohiromiyake Scopus Author ID 55165894400 55165894400 CreatorName Miyake, Tomohiro
	NRID 1000030644455 1000030644455 ORCID 0000-0002-0832-9877 0000-0002-0832-9877 researchmap permalink shimodam shimodam Scopus Author ID 55796822600 55796822600 CreatorName Shimoda, Masafumi
	NRID 1000060948074 1000060948074 researchmap permalink y_motoyama y_motoyama Scopus Author ID 57208337832 57208337832 CreatorName Motoyama, Yuichi
	NRID 1000010283772 1000010283772 ORCID 0000-0001-8490-3650 0000-0001-8490-3650 researchmap permalink read0046454 read0046454 Scopus Author ID 7003914326 7003914326 CreatorName Morii, Eiichi
	NRID 1000000403098 1000000403098 CreatorName Tanaka, Katsunori
	NRID 1000030448039 1000030448039 ORCID 0000-0002-0837-2357 0000-0002-0837-2357 researchmap permalink _-23f _-23f Scopus Author ID 7005224756 7005224756 CreatorName Shimazu, Kenzo
Subject	acrolein
	biopsy
	click-to-sense
	fine-needle
	mammary neoplasms
Description	Kitahara Y., Tanei T., Hatano T., et al. Rapid and Accurate Diagnosis of Breast Cancer by Fine-Needle Aspiration Biopsy Using the “Click-to-Sense” Method. Cancer Medicine 15, e71525 (2026); https://doi.org/10.1002/cam4.71525.
Abstract	Background: We have previously demonstrated the value of the “click-to-sense” (CTS) assay, in which a fluorescent probe targeting acrolein can detect cancer cells and differentiate between malignant and benign lesions in breast tissue. In this study, we assessed the usefulness of the CTS assay for diagnosis of breast tumors by fine-needle aspiration biopsy (FNAB). Methods: A total of 126 FNABs were performed on live tissue samples obtained by surgery (63 breast cancers, 31 benign breast tumors, and 32 normal breast glands). CTS reagents (CTS probe and Hoechst dye mixed with encapsulating agents) were added to the aspirated cells and placed on slides, which were then cover-slipped and imaged under a fluorescence microscope. Another FNAB slide was prepared for each of the same live tissue samples, fixed in ethanol, and subjected to Papanicolaou (PAP) staining. The diagnostic accuracy of the CTS assay was compared with that of PAP staining by histopathological examination of permanent sections. Results: The CTS assay had a sensitivity of 92.1%, a specificity of 96.8%, and an accuracy of 94.4% (119/126 samples); the respective values for PAP staining were 98.4%, 89.8%, and 94.2% (114/121 samples). The insufficiency/inadequacy rate was 0% for the CTS assay and 4% for PAP staining (5/126 samples). Conclusion: The CTS assay is as accurate as PAP staining for FNAB of breast lesions. This assay has the potential to establish a new diagnostic method in cytopathology in the future, because it has a lower inadequacy rate and is simpler and less labor-intensive and time-consuming to perform.
Publisher	John Wiley and Sons Inc
Source Title	Cancer Medicine
Volume (Issue)	15(2)
Page	e71525
Date of Issued	2026-02-13
Language	English
Handle URL	https://hdl.handle.net/11094/104243
eISSN	20457634
Relation.isIdenticalTo	DOI (Publisher Version) https://doi.org/10.1002/cam4.71525
Access Rights	open access
CopyRight	This article is licensed under a Creative Commons Attribution 4.0 International License.
oaire:version	Version of Record
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCMI Type	Text
DCTERMS.bibliographicCitation	Cancer Medicine. 2026, 15(2), p. e71525
DC.title	Rapid and Accurate Diagnosis of Breast Cancer by Fine-Needle Aspiration Biopsy Using the “Click-to-Sense” Method
DC.creator	Kitahara, Yuri
	Tanei, Tomonori
	Hatano, Takaaki
	Pradipta, Ambara R.
	Morimoto, Koji
	Nagatomo, Tadasuke
	Abe, Kaori
	Masunaga, Nanae
	Mishima, Chieko
	Yoshinami, Tetsuhiro
	Tsukabe, Masami
	Sota, Yoshiaki
	Miyake, Tomohiro
	Shimoda, Masafumi
	Motoyama, Yuichi
	Morii, Eiichi
	Tanaka, Katsunori
	Shimazu, Kenzo
DC.publisher	John Wiley and Sons Inc
DC.language' scheme='DCTERMS.RFC1766	English
DC.type' scheme='DCTERMS.DCMIType	Text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2026-02-13
DC.identifier	https://doi.org/10.1002/cam4.71525
DC.subject	acrolein
	biopsy
	click-to-sense
	fine-needle
	mammary neoplasms
DC.description	Kitahara Y., Tanei T., Hatano T., et al. Rapid and Accurate Diagnosis of Breast Cancer by Fine-Needle Aspiration Biopsy Using the “Click-to-Sense” Method. Cancer Medicine 15, e71525 (2026); https://doi.org/10.1002/cam4.71525.
DCTERMS.abstract	Background: We have previously demonstrated the value of the “click-to-sense” (CTS) assay, in which a fluorescent probe targeting acrolein can detect cancer cells and differentiate between malignant and benign lesions in breast tissue. In this study, we assessed the usefulness of the CTS assay for diagnosis of breast tumors by fine-needle aspiration biopsy (FNAB). Methods: A total of 126 FNABs were performed on live tissue samples obtained by surgery (63 breast cancers, 31 benign breast tumors, and 32 normal breast glands). CTS reagents (CTS probe and Hoechst dye mixed with encapsulating agents) were added to the aspirated cells and placed on slides, which were then cover-slipped and imaged under a fluorescence microscope. Another FNAB slide was prepared for each of the same live tissue samples, fixed in ethanol, and subjected to Papanicolaou (PAP) staining. The diagnostic accuracy of the CTS assay was compared with that of PAP staining by histopathological examination of permanent sections. Results: The CTS assay had a sensitivity of 92.1%, a specificity of 96.8%, and an accuracy of 94.4% (119/126 samples); the respective values for PAP staining were 98.4%, 89.8%, and 94.2% (114/121 samples). The insufficiency/inadequacy rate was 0% for the CTS assay and 4% for PAP staining (5/126 samples). Conclusion: The CTS assay is as accurate as PAP staining for FNAB of breast lesions. This assay has the potential to establish a new diagnostic method in cytopathology in the future, because it has a lower inadequacy rate and is simpler and less labor-intensive and time-consuming to perform.
DC.rights	This article is licensed under a Creative Commons Attribution 4.0 International License.
DC.format	104243
citation_title	Rapid and Accurate Diagnosis of Breast Cancer by Fine-Needle Aspiration Biopsy Using the “Click-to-Sense” Method
citation_author	Kitahara, Yuri
	Tanei, Tomonori
	Hatano, Takaaki
	Pradipta, Ambara R.
	Morimoto, Koji
	Nagatomo, Tadasuke
	Abe, Kaori
	Masunaga, Nanae
	Mishima, Chieko
	Yoshinami, Tetsuhiro
	Tsukabe, Masami
	Sota, Yoshiaki
	Miyake, Tomohiro
	Shimoda, Masafumi
	Motoyama, Yuichi
	Morii, Eiichi
	Tanaka, Katsunori
	Shimazu, Kenzo
citation_publisher	John Wiley and Sons Inc
citation_language	English
citation_date	2026-02-13
citation_journal_title	Cancer Medicine
citation_volume	15
citation_issue	2
citation_firstpage	e71525
citation_doi	https://doi.org/10.1002/cam4.71525
citation_public_url	https://hdl.handle.net/11094/104243

Rapid and Accurate Diagnosis of Breast Cancer by Fine-Needle Aspiration Biopsy Using the “Click-to-Sense” Method

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