ADAM5 is required for sperm-zona pellucida binding and sperm oviduct migration

Mashiko, Daisuke; Tonai, Shingo; Ikawa, Masahito

Biology of Reproduction, 2025, 114(3), 1091-1100

Number of Access:95（2026-08-02 22:13 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/104855

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Title
ADAM5 is required for sperm-zona pellucida binding and sperm oviduct migration
Creator
NRID
1000090938515 1000090938515
ORCID
0000-0003-3927-3076 0000-0003-3927-3076
researchmap permalink
mashikodaisuke mashikodaisuke
Scopus Author ID
55953432500 55953432500
CreatorName
Mashiko, Daisuke
ORCID
0000-0003-0466-2450 0000-0003-0466-2450
researchmap permalink
shingotonai shingotonai
CreatorName
Tonai, Shingo
NRID
1000020304066 1000020304066
ORCID
0000-0001-9859-6217 0000-0001-9859-6217
researchmap permalink
read0054910 read0054910
Scopus Author ID
16689054500 16689054500
CreatorName
Ikawa, Masahito
Subject
knockout mouse
male infertility
uterotubal junction
zona pellucida binding
Description
This is a submitted version of an article in Biology of Reproduction. The version of record is available online at: https://doi.org/10.1093/biolre/ioaf254.
Abstract
ADAM5 is a testis-specific transmembrane protein whose role in male fertility remains poorly understood. In this study, we report that Adam5 knockout (KO) male mice are severely subfertile, despite exhibiting normal testicular morphology, sperm structure, and motility. Adam5 KO sperm failed to transit the uterotubal junction (UTJ) and displayed severe defects in zona pellucida (ZP) binding, phenotypes that resemble those observed in Adam2 and Adam3 knockout mice. Western blot analysis revealed a significant reduction in the levels of ADAM2 and ADAM3 in Adam5 KO spermatozoa, supporting the previous finding that ADAM5 interacts with these proteins to form a complex. Additionally, Adam5 KO spermatozoa exhibited reduced binding to extracellular matrix (ECM) components, including Laminin I and Fibronectin. These findings suggest that ADAM5 plays a crucial role in sperm ECM interaction, a process likely critical for successful UTJ transit and ZP binding. While ADAM5 is a pseudogene in humans, our results provide valuable insights into the function of ADAM family proteins in mammalian reproduction.
Publisher
Oxford University Press
Source Title
Biology of Reproduction
Volume (Issue)
114(3)
Page
1091-1100
Date of Issued
2025-11-20
Language
English
Handle URL
https://hdl.handle.net/11094/104855
PISSN
00063363
eISSN
15297268
Relation.isVersionOf
DOI (Publisher Version)
https://doi.org/10.1093/biolre/ioaf254
Access Rights
open access
oaire:version
Author’s Original
Category
学術雑誌論文 Journal Article