Defects in Axonal Elongation and Neuronal Migration in Mice with Disrupted tau and map1b Genes

Takei, Yosuke; Teng, Junlin; Harada, Akihiro et al.

Journal of Cell Biology, 2000, 150(5), 989-1000

Number of Access:597（2025-07-27 23:38 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/23081

Link to primary information 

File Format Terms of use Size Views Date.Available Description information
jcb_150_5_989 pdf None 1.05 MB 407 2012.11.07  

Item Information

Output File Export EndNote Basic Export Mendeley

Title
Defects in Axonal Elongation and Neuronal Migration in Mice with Disrupted tau and map1b Genes
Creator
NRID
1000020272487 1000020272487
CreatorName
Takei, Yosuke
CreatorName
Teng, Junlin
NRID
1000040251441 1000040251441
researchmap permalink
aharada aharada
Scopus Author ID
7202253419 7202253419
CreatorName
Harada, Akihiro
NRID
1000020010085 1000020010085
CreatorName
Hirokawa, Nobutaka
Subject
tau
MAP1B
axon
growth cone
neuronal migration
Abstract
Tau and MAP1B are the main members of neuronal microtubule-associated proteins (MAPs), the functions of which have remained obscure because of a putative functional redundancy (Harada, A., K. Oguchi, S. Okabe, J. Kuno, S. Terada, T. Ohshima, R. SatoYoshitake, Y. Takei, T. Noda, and N. Hirokawa. 1994. Nature. 369:488–491; Takei, Y., S. Kondo, A. Harada, S. Inomata, T. Noda, and N. Hirokawa. 1997. J. Cell Biol. 137:1615–1626). To unmask the role of these proteins, we generated double-knockout mice with disrupted tau and map1b genes and compared their phenotypes with those of single-knockout mice. In the analysis of mice with a genetic background of predominantly C57Bl/6J, a hypoplastic commissural axon tract and disorganized neuronal layering were observed in the brains of the tau +/+map1b-/-mice. These phenotypes are markedly more severe in tau -/-map1b-/-double mutants, indicating that tau and MAP1B act in a synergistic fashion. Primary cultures of hippocampal neurons from tau -/-map1b-/-mice showed inhibited axonal elongation. In these cells, a generation of new axons via bundling of microtubules at the neck of the growth cones appeared to be disturbed. Cultured cerebellar neurons from tau -/-map1b-/-mice showed delayed neuronal migration concomitant with suppressed neurite elongation. These findings indicate the cooperative functions of tau and MAP1B in vivo in axonal elongation and neuronal migration as regulators of microtubule oganization.
Publisher
The Rockefeller University Press
Source Title
Journal of Cell Biology
Volume (Issue)
150(5)
Page
989-1000
Date of Issued
2000-09-04
Language
English
Handle URL
https://hdl.handle.net/11094/23081
PISSN
00219525
NCID
AA00694812
Relation.isIdenticalTo
DOI (Publisher Version)
https://doi.org/10.1083/jcb.150.5.989
Access Rights
open access
oaire:version
Version of Record
Category
学術雑誌論文 Journal Article