Caenorhabditis elegans SNAP-29 is required for organellar integrity of the endomembrane system and general exocytosis in intestinal epithelial cells

Sato, Miyuki; Saegusa, Keiko; Sato, Katsuya; Hara, Taichi; Harada, Akihiro; Sato, Ken

doi:https://doi.org/10.1091/mbc.E11-04-0279

Caenorhabditis elegans SNAP-29 is required for organellar integrity of the endomembrane system and general exocytosis in intestinal epithelial cells

Sato, Miyuki; Saegusa, Keiko; Sato, Katsuya et al.

Molecular Biology of the Cell, 2011, 22(14), 2579-2587

Number of Access:800（2025-07-27 23:42 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/23086

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Title	Caenorhabditis elegans SNAP-29 is required for organellar integrity of the endomembrane system and general exocytosis in intestinal epithelial cells
Creator	NRID 1000070321768 1000070321768 CreatorName Sato, Miyuki
	CreatorName Saegusa, Keiko
	NRID 1000090370402 1000090370402 CreatorName Sato, Katsuya
	NRID 1000000392374 1000000392374 CreatorName Hara, Taichi
	NRID 1000040251441 1000040251441 researchmap permalink aharada aharada Scopus Author ID 7202253419 7202253419 CreatorName Harada, Akihiro
	CreatorName Sato, Ken
Abstract	It is generally accepted that soluble N-ethylmaleimide–sensitive factor attachment protein receptors mediate the docking and fusion of transport intermediates with target membranes. Our research identifies Caenorhabditis elegans homologue of synaptosomal-associated protein 29 (SNAP-29) as an essential regulator of membrane trafficking in polarized intestinal cells of living animals. We show that a depletion of SNAP-29 blocks yolk secretion and targeting of apical and basolateral plasma membrane proteins in the intestinal cells and results in a strong accumulation of small cargo-containing vesicles. The loss of SNAP-29 also blocks the transport of yolk receptor RME-2 to the plasma membrane in nonpolarized oocytes, indicating that its function is required in various cell types. SNAP-29 is essential for embryogenesis, animal growth, and viability. Functional fluorescent protein–tagged SNAP-29 mainly localizes to the plasma membrane and the late Golgi, although it also partially colocalizes with endosomal proteins. The loss of SNAP-29 leads to the vesiculation/fragmentation of the Golgi and endosomes, suggesting that SNAP-29 is involved in multiple transport pathways between the exocytic and endocytic organelles. These observations also suggest that organelles comprising the endomembrane system are highly dynamic structures based on the balance between membrane budding and fusion and that SNAP-29–mediated fusion is required to maintain proper organellar morphology and functions.
Publisher	The American Society for Cell Biology
Source Title	Molecular Biology of the Cell
Volume (Issue)	22(14)
Page	2579-2587
Date of Issued	2011-07-15
Language	English
Handle URL	https://hdl.handle.net/11094/23086
PISSN	10591524
NCID	AA10830484
Relation.references	URI http://www.molbiolcell.org/content/22/14/2579.full
Relation.isIdenticalTo	DOI (Publisher Version) https://doi.org/10.1091/mbc.E11-04-0279
Access Rights	open access
oaire:version	Version of Record
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCMI Type	Text
DCTERMS.bibliographicCitation	Molecular Biology of the Cell. 2011, 22(14), p. 2579-2587
DC.title	Caenorhabditis elegans SNAP-29 is required for organellar integrity of the endomembrane system and general exocytosis in intestinal epithelial cells
DC.creator	Sato, Miyuki
	Saegusa, Keiko
	Sato, Katsuya
	Hara, Taichi
	Harada, Akihiro
	Sato, Ken
DC.publisher	The American Society for Cell Biology
DC.language' scheme='DCTERMS.RFC1766	English
DC.type' scheme='DCTERMS.DCMIType	Text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2011-07-15
DC.identifier	https://doi.org/10.1091/mbc.E11-04-0279
DC.relation' scheme='DCTERMS.URI	http://www.molbiolcell.org/content/22/14/2579.full
DCTERMS.abstract	It is generally accepted that soluble N-ethylmaleimide–sensitive factor attachment protein receptors mediate the docking and fusion of transport intermediates with target membranes. Our research identifies Caenorhabditis elegans homologue of synaptosomal-associated protein 29 (SNAP-29) as an essential regulator of membrane trafficking in polarized intestinal cells of living animals. We show that a depletion of SNAP-29 blocks yolk secretion and targeting of apical and basolateral plasma membrane proteins in the intestinal cells and results in a strong accumulation of small cargo-containing vesicles. The loss of SNAP-29 also blocks the transport of yolk receptor RME-2 to the plasma membrane in nonpolarized oocytes, indicating that its function is required in various cell types. SNAP-29 is essential for embryogenesis, animal growth, and viability. Functional fluorescent protein–tagged SNAP-29 mainly localizes to the plasma membrane and the late Golgi, although it also partially colocalizes with endosomal proteins. The loss of SNAP-29 leads to the vesiculation/fragmentation of the Golgi and endosomes, suggesting that SNAP-29 is involved in multiple transport pathways between the exocytic and endocytic organelles. These observations also suggest that organelles comprising the endomembrane system are highly dynamic structures based on the balance between membrane budding and fusion and that SNAP-29–mediated fusion is required to maintain proper organellar morphology and functions.
DC.format	23086
citation_title	Caenorhabditis elegans SNAP-29 is required for organellar integrity of the endomembrane system and general exocytosis in intestinal epithelial cells
citation_author	Sato, Miyuki
	Saegusa, Keiko
	Sato, Katsuya
	Hara, Taichi
	Harada, Akihiro
	Sato, Ken
citation_publisher	The American Society for Cell Biology
citation_language	English
citation_date	2011-07-15
citation_journal_title	Molecular Biology of the Cell
citation_volume	22
citation_issue	14
citation_firstpage	2579
citation_lastpage	2587
citation_doi	https://doi.org/10.1091/mbc.E11-04-0279
citation_public_url	https://hdl.handle.net/11094/23086
citation_abstract_html_url	http://www.molbiolcell.org/content/22/14/2579.full

Caenorhabditis elegans SNAP-29 is required for organellar integrity of the endomembrane system and general exocytosis in intestinal epithelial cells

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