Delayed Development of Nervous System in Mice Homozygous for Disrupted Microtubule-associated Protein 1B (MAP1B) Gene

Takei, Yosuke; Kondo, Satoru; Harada, Akihiro; Inomata, Satomi; Noda, Tetsuo; Hirokawa, Nobutaka

doi:https://doi.org/10.1083/jcb.137.7.1615

Delayed Development of Nervous System in Mice Homozygous for Disrupted Microtubule-associated Protein 1B (MAP1B) Gene

Takei, Yosuke; Kondo, Satoru; Harada, Akihiro et al.

Journal of Cell Biology, 1997, 137(7), 1615-1626

Number of Access:693（2025-07-27 23:29 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/23091

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Title	Delayed Development of Nervous System in Mice Homozygous for Disrupted Microtubule-associated Protein 1B (MAP1B) Gene
Creator	NRID 1000020272487 1000020272487 CreatorName Takei, Yosuke
	NRID 1000060763737 1000060763737 CreatorName Kondo, Satoru
	NRID 1000040251441 1000040251441 researchmap permalink aharada aharada Scopus Author ID 7202253419 7202253419 CreatorName Harada, Akihiro
	CreatorName Inomata, Satomi
	NRID 1000010183550 1000010183550 CreatorName Noda, Tetsuo
	NRID 1000020010085 1000020010085 CreatorName Hirokawa, Nobutaka
Abstract	Microtubule-associated protein 1B (MAP1B), one of the microtubule-associated proteins (MAPs), is a major component of the neuronal cytoskeleton. It is expressed at high levels in immature neurons during growth of their axons, which indicates that it plays a crucial role in neuronal morphogenesis and neurite extension. To better define the role of MAP1B in vivo, we have used gene targeting to disrupt the murine MAP1B gene. Heterozygotes of our MAP1B disruption exhibit no overt abnormalities in their development and behavior, while homozygotes showed a slightly decreased brain weight and delayed nervous system development. Our data indicate that while MAP1B is not essential for survival, it is essential for normal time course development of the murine nervous system. These conclusions are very different from those of a previous MAP1B gene–targeting study (Edelmann, W., M. Zervas, P. Costello, L. Roback, I. Fischer, A. Hammarback, N. Cowan, P. Davis, B. Wainer, and R. Kucherlapati. 1996. Proc. Natl. Acad. Sci. USA. 93: 1270–1275). In this previous effort, homozygotes died before reaching 8-d embryos, while heterozygotes showed severely abnormal phenotypes in their nervous systems. Because the gene targeting event in these mice produced a gene encoding a 571–amino acid truncated product of MAP1B, it seems likely that the phenotypes seen arise from the truncated MAP1B product acting in a dominant-negative fashion, rather than a loss of MAP1B function.
Publisher	The Rockefeller University Press
Source Title	Journal of Cell Biology
Volume (Issue)	137(7)
Page	1615-1626
Date of Issued	1997-06-30
Language	English
Handle URL	https://hdl.handle.net/11094/23091
PISSN	00219525
NCID	AA00694812
Relation.isIdenticalTo	DOI (Publisher Version) https://doi.org/10.1083/jcb.137.7.1615
Access Rights	open access
oaire:version	Version of Record
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCMI Type	Text
DCTERMS.bibliographicCitation	Journal of Cell Biology. 1997, 137(7), p. 1615-1626
DC.title	Delayed Development of Nervous System in Mice Homozygous for Disrupted Microtubule-associated Protein 1B (MAP1B) Gene
DC.creator	Takei, Yosuke
	Kondo, Satoru
	Harada, Akihiro
	Inomata, Satomi
	Noda, Tetsuo
	Hirokawa, Nobutaka
DC.publisher	The Rockefeller University Press
DC.language' scheme='DCTERMS.RFC1766	English
DC.type' scheme='DCTERMS.DCMIType	Text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	1997-06-30
DC.identifier	https://doi.org/10.1083/jcb.137.7.1615
DCTERMS.abstract	Microtubule-associated protein 1B (MAP1B), one of the microtubule-associated proteins (MAPs), is a major component of the neuronal cytoskeleton. It is expressed at high levels in immature neurons during growth of their axons, which indicates that it plays a crucial role in neuronal morphogenesis and neurite extension. To better define the role of MAP1B in vivo, we have used gene targeting to disrupt the murine MAP1B gene. Heterozygotes of our MAP1B disruption exhibit no overt abnormalities in their development and behavior, while homozygotes showed a slightly decreased brain weight and delayed nervous system development. Our data indicate that while MAP1B is not essential for survival, it is essential for normal time course development of the murine nervous system. These conclusions are very different from those of a previous MAP1B gene–targeting study (Edelmann, W., M. Zervas, P. Costello, L. Roback, I. Fischer, A. Hammarback, N. Cowan, P. Davis, B. Wainer, and R. Kucherlapati. 1996. Proc. Natl. Acad. Sci. USA. 93: 1270–1275). In this previous effort, homozygotes died before reaching 8-d embryos, while heterozygotes showed severely abnormal phenotypes in their nervous systems. Because the gene targeting event in these mice produced a gene encoding a 571–amino acid truncated product of MAP1B, it seems likely that the phenotypes seen arise from the truncated MAP1B product acting in a dominant-negative fashion, rather than a loss of MAP1B function.
DC.format	23091
citation_title	Delayed Development of Nervous System in Mice Homozygous for Disrupted Microtubule-associated Protein 1B (MAP1B) Gene
citation_author	Takei, Yosuke
	Kondo, Satoru
	Harada, Akihiro
	Inomata, Satomi
	Noda, Tetsuo
	Hirokawa, Nobutaka
citation_publisher	The Rockefeller University Press
citation_language	English
citation_date	1997-06-30
citation_journal_title	Journal of Cell Biology
citation_volume	137
citation_issue	7
citation_firstpage	1615
citation_lastpage	1626
citation_doi	https://doi.org/10.1083/jcb.137.7.1615
citation_public_url	https://hdl.handle.net/11094/23091

Delayed Development of Nervous System in Mice Homozygous for Disrupted Microtubule-associated Protein 1B (MAP1B) Gene

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