Self-assembly of the chaperonin GroEL nanocage induced at submicellar detergent

Chen, Jin; Yagi, Hisashi; Furutani, Yuji et al.

Scientific Reports, 2014, 4, 5614

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Identifier to cite or link to this item: https://hdl.handle.net/11094/71271

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Title
Self-assembly of the chaperonin GroEL nanocage induced at submicellar detergent
Creator
CreatorName
Chen, Jin
NRID
1000010432494 1000010432494
CreatorName
Yagi, Hisashi
NRID
1000080432285 1000080432285
CreatorName
Furutani, Yuji
CreatorName
Nakamura, Takashi
CreatorName
Inaguma, Asumi
CreatorName
Guo, Hao
CreatorName
Kong, Yan
NRID
1000040153770 1000040153770
researchmap permalink
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CreatorName
Goto, Yuji
Description
Jin Chen, Hisashi Yagi, Yuji Furutani, Takashi Nakamura, Asumi Inaguma, Hao Guo, Yan Kong & Yuji Goto "Self-assembly of the chaperonin GroEL nanocage induced at submicellar detergent", Scientific Reports, volume 4, Article number: 5614, Springer Nature, 2014
Abstract
Protein nanoassemblies possess unique advantage in biomedical applications such as drug delivery, biocatalysis and vaccine development. Despite recent accomplishment in atomic structure data, the underlying molecular mechanism of protein self-assembly remains elusive, where considerable heterogeneity is often involved. Here we use E. coli chaperonin GroEL, a tetradecameric protein with a molecular weight of 805 kDa, to probe its transformation from cage-like oligomers to protein nanofibers. We show that sodium dodecyl sulfate (SDS), a widely-used protein denaturant, at submicellar concentration binds to and causes partial distortion of GroEL apical domain. Subsequently, the GroEL apical domain with altered secondary structural content converts the GroEL oligomers into modular structural units which are observed to self-assemble into cylindrical nanofibers under an agitated incubation in a physiological buffer. Interestingly, through targeted mutagenesis where two cysteine residues are introduced at the entry site of GroEL cage, we found that the formation of GroEL nanoassembly could be modulated depending on the redox condition of incubation. Without the need of chemical engineering, tunable GroEL nanofibers built by controlled-assembly are among the largest nanoscale bioassembly with broad applications.
Publisher
Springer Nature
Source Title
Scientific Reports
Volume (Issue)
4
Page
5614
Date of Issued
2014-07
Language
English
Handle URL
https://hdl.handle.net/11094/71271
eISSN
20452322
Relation.isIdenticalTo
DOI (Publisher Version)
https://doi.org/10.1038/srep05614
Access Rights
open access
CopyRight
© 2014 The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License.
oaire:version
Version of Record
Category
学術雑誌論文 Journal Article