Destruction of Amyloid Fibrils of Keratoepithelin Peptides by Laser Irradiation Coupled with Amyloid-specific Thioflavin T

Ozawa, Daisaku; Kaji, Yuichi; Yagi, Hisashi; Sakurai, Kazumasa; Kawakami, Toru; Naiki, Hironobu; Goto, Yuji

doi:https://doi.org/10.1074/jbc.M111.222901

Destruction of Amyloid Fibrils of Keratoepithelin Peptides by Laser Irradiation Coupled with Amyloid-specific Thioflavin T

Ozawa, Daisaku; Kaji, Yuichi; Yagi, Hisashi et al.

Journal of Biological Chemistry, 2011, 286(12), 10856-10863

Number of Access:738（2025-08-30 01:43 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/71284

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Title	Destruction of Amyloid Fibrils of Keratoepithelin Peptides by Laser Irradiation Coupled with Amyloid-specific Thioflavin T
Creator	NRID 1000060554524 1000060554524 CreatorName Ozawa, Daisaku
	NRID 1000050361332 1000050361332 CreatorName Kaji, Yuichi
	NRID 1000010432494 1000010432494 CreatorName Yagi, Hisashi
	NRID 1000010403015 1000010403015 CreatorName Sakurai, Kazumasa
	NRID 1000070273711 1000070273711 CreatorName Kawakami, Toru
	NRID 1000010227704 1000010227704 CreatorName Naiki, Hironobu
	NRID 1000040153770 1000040153770 researchmap permalink read0014440 read0014440 CreatorName Goto, Yuji
Description	This research was originally published in the Journal of Biological Chemistry. Daisaku Ozawa, Yuichi Kaji, Hisashi Yagi, Kazumasa Sakurai, Toru Kawakami, Hironobu Naiki, and Yuji Goto. Destruction of Amyloid Fibrils of Keratoepithelin Peptides byLaser Irradiation Coupled with Amyloid-specific Thioflavin T. J. Biol. Chem. 2011; 286, 10856-10863. © the American Society for Biochemistry and Molecular Biology
Abstract	Mutations in keratoepithelin are associated with blinding ocular diseases, including lattice corneal dystrophy type 1 and granular corneal dystrophy type 2. These diseases are characterized by deposits of amyloid fibrils and/or granular non-amyloid aggregates in the cornea. Removing the deposits in the cornea is important for treatment. Previously, we reported the destruction of amyloid fibrils of β2-microglobulin K3 fragments and amyloid β by laser irradiation coupled with the binding of an amyloid-specific thioflavin T. Here, we studied the effects of this combination on the amyloid fibrils of two 22-residue fragments of keratoepithelin. The direct observation of individual amyloid fibrils was performed in real time using total internal reflection fluorescence microscopy. Both types of amyloid fibrils were broken up by the laser irradiation, dependent on the laser power. The results suggest the laser-induced destruction of amyloid fibrils to be a useful strategy for the treatment of these corneal dystrophies.
Publisher	American Society for Biochemistry and Molecular Biology
Source Title	Journal of Biological Chemistry
Volume (Issue)	286(12)
Page	10856-10863
Date of Issued	2011-03
Language	English
Handle URL	https://hdl.handle.net/11094/71284
eISSN	1083351X
NCID	AA1202453X
Relation.isIdenticalTo	DOI (Publisher Version) https://doi.org/10.1074/jbc.M111.222901
Access Rights	open access
oaire:version	Version of Record
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCMI Type	Text
DCTERMS.bibliographicCitation	Journal of Biological Chemistry. 2011, 286(12), p. 10856-10863
DC.title	Destruction of Amyloid Fibrils of Keratoepithelin Peptides by Laser Irradiation Coupled with Amyloid-specific Thioflavin T
DC.creator	Ozawa, Daisaku
	Kaji, Yuichi
	Yagi, Hisashi
	Sakurai, Kazumasa
	Kawakami, Toru
	Naiki, Hironobu
	Goto, Yuji
DC.publisher	American Society for Biochemistry and Molecular Biology
DC.language' scheme='DCTERMS.RFC1766	English
DC.type' scheme='DCTERMS.DCMIType	Text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2011-03
DC.identifier	https://doi.org/10.1074/jbc.M111.222901
DC.description	This research was originally published in the Journal of Biological Chemistry. Daisaku Ozawa, Yuichi Kaji, Hisashi Yagi, Kazumasa Sakurai, Toru Kawakami, Hironobu Naiki, and Yuji Goto. Destruction of Amyloid Fibrils of Keratoepithelin Peptides byLaser Irradiation Coupled with Amyloid-specific Thioflavin T. J. Biol. Chem. 2011; 286, 10856-10863. © the American Society for Biochemistry and Molecular Biology
DCTERMS.abstract	Mutations in keratoepithelin are associated with blinding ocular diseases, including lattice corneal dystrophy type 1 and granular corneal dystrophy type 2. These diseases are characterized by deposits of amyloid fibrils and/or granular non-amyloid aggregates in the cornea. Removing the deposits in the cornea is important for treatment. Previously, we reported the destruction of amyloid fibrils of β2-microglobulin K3 fragments and amyloid β by laser irradiation coupled with the binding of an amyloid-specific thioflavin T. Here, we studied the effects of this combination on the amyloid fibrils of two 22-residue fragments of keratoepithelin. The direct observation of individual amyloid fibrils was performed in real time using total internal reflection fluorescence microscopy. Both types of amyloid fibrils were broken up by the laser irradiation, dependent on the laser power. The results suggest the laser-induced destruction of amyloid fibrils to be a useful strategy for the treatment of these corneal dystrophies.
DC.format	71284
citation_title	Destruction of Amyloid Fibrils of Keratoepithelin Peptides by Laser Irradiation Coupled with Amyloid-specific Thioflavin T
citation_author	Ozawa, Daisaku
	Kaji, Yuichi
	Yagi, Hisashi
	Sakurai, Kazumasa
	Kawakami, Toru
	Naiki, Hironobu
	Goto, Yuji
citation_publisher	American Society for Biochemistry and Molecular Biology
citation_language	English
citation_date	2011-03
citation_journal_title	Journal of Biological Chemistry
citation_volume	286
citation_issue	12
citation_firstpage	10856
citation_lastpage	10863
citation_doi	https://doi.org/10.1074/jbc.M111.222901
citation_public_url	https://hdl.handle.net/11094/71284

Destruction of Amyloid Fibrils of Keratoepithelin Peptides by Laser Irradiation Coupled with Amyloid-specific Thioflavin T

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