Laser-induced Propagation and Destruction of Amyloid β Fibrils

Yagi, Hisashi; Ozawa, Daisaku; Sakurai, Kazumasa; Kawakami, Toru; Kuyama, Hiroki; Nishimura, Osamu; Shimanouchi, Toshinori; Kuboi, Ryoichi; Naiki, Hironobu; Goto, Yuji

doi:https://doi.org/10.1074/jbc.M109.076505

Laser-induced Propagation and Destruction of Amyloid β Fibrils

Yagi, Hisashi; Ozawa, Daisaku; Sakurai, Kazumasa et al.

Journal of Biological Chemistry, 2010, 285(25), 19660-19667

Number of Access:1,012（2025-12-06 23:22 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/71286

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Title	Laser-induced Propagation and Destruction of Amyloid β Fibrils
Creator	NRID 1000010432494 1000010432494 CreatorName Yagi, Hisashi
	NRID 1000060554524 1000060554524 CreatorName Ozawa, Daisaku
	NRID 1000010403015 1000010403015 CreatorName Sakurai, Kazumasa
	NRID 1000070273711 1000070273711 CreatorName Kawakami, Toru
	NRID 1000060437332 1000060437332 CreatorName Kuyama, Hiroki
	NRID 1000010374125 1000010374125 CreatorName Nishimura, Osamu
	NRID 1000010335383 1000010335383 CreatorName Shimanouchi, Toshinori
	NRID 1000040029567 1000040029567 CreatorName Kuboi, Ryoichi
	NRID 1000010227704 1000010227704 CreatorName Naiki, Hironobu
	NRID 1000040153770 1000040153770 researchmap permalink read0014440 read0014440 CreatorName Goto, Yuji
Description	This research was originally published in the Journal of Biological Chemistry. Hisashi Yagi, Daisaku Ozawa, Kazumasa Sakurai, Toru Kawakami, Hiroki Kuyama, Osamu Nishimura,Toshinori Shimanouchi, Ryoichi Kuboi, Hironobu Naiki, and Yuji Goto. Laser-induced Propagation and Destruction of Amyloid β Fibrils. J. Biol. Chem. 2010; 285, 19660-19667. © the American Society for Biochemistry and Molecular Biology
Abstract	The amyloid deposition of amyloid β (Aβ) peptides is a critical pathological event in Alzheimer disease (AD). Preventing the formation of amyloid deposits and removing preformed fibrils in tissues are important therapeutic strategies against AD. Previously, we reported the destruction of amyloid fibrils of β2-microglobulin K3 fragments by laser irradiation coupled with the binding of amyloid-specific thioflavin T. Here, we studied the effects of a laser beam on Aβ fibrils. As was the case for K3 fibrils, extensive irradiation destroyed the preformed Aβ fibrils. However, irradiation during spontaneous fibril formation resulted in only the partial destruction of growing fibrils and a subsequent explosive propagation of fibrils. The explosive propagation was caused by an increase in the number of active ends due to breakage. The results not only reveal a case of fragmentation-induced propagation of fibrils but also provide insights into therapeutic strategies for AD.
Publisher	American Society for Biochemistry and Molecular Biology
Source Title	Journal of Biological Chemistry
Volume (Issue)	285(25)
Page	19660-19667
Date of Issued	2010-06
Language	English
Handle URL	https://hdl.handle.net/11094/71286
eISSN	1083351X
NCID	AA1202455X
Relation.isIdenticalTo	DOI (Publisher Version) https://doi.org/10.1074/jbc.M109.076505
Access Rights	open access
oaire:version	Version of Record
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCMI Type	Text
DCTERMS.bibliographicCitation	Journal of Biological Chemistry. 2010, 285(25), p. 19660-19667
DC.title	Laser-induced Propagation and Destruction of Amyloid β Fibrils
DC.creator	Yagi, Hisashi
	Ozawa, Daisaku
	Sakurai, Kazumasa
	Kawakami, Toru
	Kuyama, Hiroki
	Nishimura, Osamu
	Shimanouchi, Toshinori
	Kuboi, Ryoichi
	Naiki, Hironobu
	Goto, Yuji
DC.publisher	American Society for Biochemistry and Molecular Biology
DC.language' scheme='DCTERMS.RFC1766	English
DC.type' scheme='DCTERMS.DCMIType	Text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2010-06
DC.identifier	https://doi.org/10.1074/jbc.M109.076505
DC.description	This research was originally published in the Journal of Biological Chemistry. Hisashi Yagi, Daisaku Ozawa, Kazumasa Sakurai, Toru Kawakami, Hiroki Kuyama, Osamu Nishimura,Toshinori Shimanouchi, Ryoichi Kuboi, Hironobu Naiki, and Yuji Goto. Laser-induced Propagation and Destruction of Amyloid β Fibrils. J. Biol. Chem. 2010; 285, 19660-19667. © the American Society for Biochemistry and Molecular Biology
DCTERMS.abstract	The amyloid deposition of amyloid β (Aβ) peptides is a critical pathological event in Alzheimer disease (AD). Preventing the formation of amyloid deposits and removing preformed fibrils in tissues are important therapeutic strategies against AD. Previously, we reported the destruction of amyloid fibrils of β2-microglobulin K3 fragments by laser irradiation coupled with the binding of amyloid-specific thioflavin T. Here, we studied the effects of a laser beam on Aβ fibrils. As was the case for K3 fibrils, extensive irradiation destroyed the preformed Aβ fibrils. However, irradiation during spontaneous fibril formation resulted in only the partial destruction of growing fibrils and a subsequent explosive propagation of fibrils. The explosive propagation was caused by an increase in the number of active ends due to breakage. The results not only reveal a case of fragmentation-induced propagation of fibrils but also provide insights into therapeutic strategies for AD.
DC.format	71286
citation_title	Laser-induced Propagation and Destruction of Amyloid β Fibrils
citation_author	Yagi, Hisashi
	Ozawa, Daisaku
	Sakurai, Kazumasa
	Kawakami, Toru
	Kuyama, Hiroki
	Nishimura, Osamu
	Shimanouchi, Toshinori
	Kuboi, Ryoichi
	Naiki, Hironobu
	Goto, Yuji
citation_publisher	American Society for Biochemistry and Molecular Biology
citation_language	English
citation_date	2010-06
citation_journal_title	Journal of Biological Chemistry
citation_volume	285
citation_issue	25
citation_firstpage	19660
citation_lastpage	19667
citation_doi	https://doi.org/10.1074/jbc.M109.076505
citation_public_url	https://hdl.handle.net/11094/71286

Laser-induced Propagation and Destruction of Amyloid β Fibrils

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