Recombinant Expression and Characterization of a Novel Fibronectin Isoform Expressed in Cartilaginous Tissues

Kozaki, Tomohiro; Matsui, Yoshito; Gu, Jianguo; Nishiuchi, Ryoko; Sugiura, Nobuo; Kimata, Koji; Ozono, Keiichi; Yoshikawa, Hideki; Sekiguchi, Kiyotoshi

doi:https://doi.org/10.1074/jbc.M307432200

Recombinant Expression and Characterization of a Novel Fibronectin Isoform Expressed in Cartilaginous Tissues

Kozaki, Tomohiro; Matsui, Yoshito; Gu, Jianguo et al.

Journal of Biological Chemistry, 2003, 278(50), 50546-50553

Number of Access:967（2025-08-18 14:07 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/71432

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Title	Recombinant Expression and Characterization of a Novel Fibronectin Isoform Expressed in Cartilaginous Tissues
Creator	CreatorName Kozaki, Tomohiro
	CreatorName Matsui, Yoshito
	NRID 1000040260369 1000040260369 CreatorName Gu, Jianguo
	CreatorName Nishiuchi, Ryoko
	NRID 1000090454420 1000090454420 CreatorName Sugiura, Nobuo
	NRID 1000010022641 1000010022641 CreatorName Kimata, Koji
	NRID 1000020270770 1000020270770 researchmap permalink read0092357 read0092357 Scopus Author ID 55545335700 55545335700 CreatorName Ozono, Keiichi
	NRID 1000060191558 1000060191558 CreatorName Yoshikawa, Hideki
	NRID 1000050187845 1000050187845 researchmap permalink read0054833 read0054833 Scopus Author ID 7201662709 7201662709 ORCID 0000-0002-6433-1410 0000-0002-6433-1410 CreatorName Sekiguchi, Kiyotoshi
Description	This research was originally published in the Journal of Biological Chemistry. Tomohiro Kozaki, Yoshito Matsui, Jianguo Gu, Ryoko Nishiuchi, Nobuo Sugiura, Koji Kimata, Keiichi Ozono, Hideki Yoshikawa and Kiyotoshi Sekiguchi. Recombinant Expression and Characterization of a Novel Fibronectin Isoform Expressed in Cartilaginous Tissues. J. Biol. Chem. 2003; 278: 50546-50553 © the American Society for Biochemistry and Molecular Biology
Abstract	A novel fibronectin (FN) isoform lacking the segment from IIICS (type III connecting segment) through the I-10 module is expressed predominantly in normal cartilaginous tissues. We expressed and purified recombinant cartilage-type FN using a mammalian expression system and characterized its molecular and biological properties. Although FNs have been shown to be secreted as disulfide-bonded dimers, cartilage-type FN was secreted mainly as a monomer. It was less potent than plasma-type FN in promoting cell adhesion and binding to integrin α5β1, although it was more active than plasma-type FN in binding to chondroitin sulfate E. When added exogenously, cartilage-type FN was poorly assembled into the fibrillar FN matrix, mostly because of its monomeric structure. Given that cartilage is characterized by its non-fibrillar matrix with abundant chondroitin sulfate-containing proteoglycans, it is likely that cartilage-type FN has evolved to adapt itself to the non-fibrillar structure of the cartilage matrix through acquisition of a novel mechanism of alternative pre-mRNA splicing.
Publisher	American Society for Biochemistry and Molecular Biology
Source Title	Journal of Biological Chemistry
Volume (Issue)	278(50)
Page	50546-50553
Date of Issued	2003-12
Language	English
Handle URL	https://hdl.handle.net/11094/71432
eISSN	1083351X
NCID	AA1202441X
Relation.isIdenticalTo	DOI (Publisher Version) https://doi.org/10.1074/jbc.M307432200
Access Rights	open access
oaire:version	Version of Record
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCMI Type	Text
DCTERMS.bibliographicCitation	Journal of Biological Chemistry. 2003, 278(50), p. 50546-50553
DC.title	Recombinant Expression and Characterization of a Novel Fibronectin Isoform Expressed in Cartilaginous Tissues
DC.creator	Kozaki, Tomohiro
	Matsui, Yoshito
	Gu, Jianguo
	Nishiuchi, Ryoko
	Sugiura, Nobuo
	Kimata, Koji
	Ozono, Keiichi
	Yoshikawa, Hideki
	Sekiguchi, Kiyotoshi
DC.publisher	American Society for Biochemistry and Molecular Biology
DC.language' scheme='DCTERMS.RFC1766	English
DC.type' scheme='DCTERMS.DCMIType	Text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2003-12
DC.identifier	https://doi.org/10.1074/jbc.M307432200
DC.description	This research was originally published in the Journal of Biological Chemistry. Tomohiro Kozaki, Yoshito Matsui, Jianguo Gu, Ryoko Nishiuchi, Nobuo Sugiura, Koji Kimata, Keiichi Ozono, Hideki Yoshikawa and Kiyotoshi Sekiguchi. Recombinant Expression and Characterization of a Novel Fibronectin Isoform Expressed in Cartilaginous Tissues. J. Biol. Chem. 2003; 278: 50546-50553 © the American Society for Biochemistry and Molecular Biology
DCTERMS.abstract	A novel fibronectin (FN) isoform lacking the segment from IIICS (type III connecting segment) through the I-10 module is expressed predominantly in normal cartilaginous tissues. We expressed and purified recombinant cartilage-type FN using a mammalian expression system and characterized its molecular and biological properties. Although FNs have been shown to be secreted as disulfide-bonded dimers, cartilage-type FN was secreted mainly as a monomer. It was less potent than plasma-type FN in promoting cell adhesion and binding to integrin α5β1, although it was more active than plasma-type FN in binding to chondroitin sulfate E. When added exogenously, cartilage-type FN was poorly assembled into the fibrillar FN matrix, mostly because of its monomeric structure. Given that cartilage is characterized by its non-fibrillar matrix with abundant chondroitin sulfate-containing proteoglycans, it is likely that cartilage-type FN has evolved to adapt itself to the non-fibrillar structure of the cartilage matrix through acquisition of a novel mechanism of alternative pre-mRNA splicing.
DC.format	71432
citation_title	Recombinant Expression and Characterization of a Novel Fibronectin Isoform Expressed in Cartilaginous Tissues
citation_author	Kozaki, Tomohiro
	Matsui, Yoshito
	Gu, Jianguo
	Nishiuchi, Ryoko
	Sugiura, Nobuo
	Kimata, Koji
	Ozono, Keiichi
	Yoshikawa, Hideki
	Sekiguchi, Kiyotoshi
citation_publisher	American Society for Biochemistry and Molecular Biology
citation_language	English
citation_date	2003-12
citation_journal_title	Journal of Biological Chemistry
citation_volume	278
citation_issue	50
citation_firstpage	50546
citation_lastpage	50553
citation_doi	https://doi.org/10.1074/jbc.M307432200
citation_public_url	https://hdl.handle.net/11094/71432

Recombinant Expression and Characterization of a Novel Fibronectin Isoform Expressed in Cartilaginous Tissues

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