Molecular Basis of Constitutive Production of Basement Membrane Components : GENE EXPRESSION PROFILES OF ENGELBRETH-HOLM-SWARM TUMOR AND F9 EMBRYONAL CARCINOMA CELLS

Futaki, Sugiko; Hayashi, Yoshitaka; Yamashita, Megumi; Yagi, Ken; Bono, Hidemasa; Hayashizaki, Yoshihide; Okazaki, Yasushi; Sekiguchi, Kiyotoshi

doi:https://doi.org/10.1074/jbc.M304985200

Molecular Basis of Constitutive Production of Basement Membrane Components : GENE EXPRESSION PROFILES OF ENGELBRETH-HOLM-SWARM TUMOR AND F9 EMBRYONAL CARCINOMA CELLS

Futaki, Sugiko; Hayashi, Yoshitaka; Yamashita, Megumi et al.

Journal of Biological Chemistry, 2003, 278(50), 50691-50701

Number of Access:654（2025-08-18 14:07 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/71433

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Title	Molecular Basis of Constitutive Production of Basement Membrane Components : GENE EXPRESSION PROFILES OF ENGELBRETH-HOLM-SWARM TUMOR AND F9 EMBRYONAL CARCINOMA CELLS
Creator	NRID 1000000403014 1000000403014 CreatorName Futaki, Sugiko
	NRID 1000080420363 1000080420363 CreatorName Hayashi, Yoshitaka
	CreatorName Yamashita, Megumi
	CreatorName Yagi, Ken
	NRID 1000020364789 1000020364789 CreatorName Bono, Hidemasa
	NRID 1000070192705 1000070192705 CreatorName Hayashizaki, Yoshihide
	NRID 1000080280733 1000080280733 CreatorName Okazaki, Yasushi
	NRID 1000050187845 1000050187845 researchmap permalink read0054833 read0054833 Scopus Author ID 7201662709 7201662709 ORCID 0000-0002-6433-1410 0000-0002-6433-1410 CreatorName Sekiguchi, Kiyotoshi
Description	This research was originally published in the Journal of Biological Chemistry. Sugiko Futaki, Yoshitaka Hayashi, Megumi Yamashita, Ken Yagi, Hidemasa Bono, Yoshihide Hayashizaki, Yasushi Okazaki and Kiyotoshi Sekiguchi. Molecular Basis of Constitutive Production of Basement Membrane Components : GENE EXPRESSION PROFILES OF ENGELBRETH-HOLM-SWARM TUMOR AND F9 EMBRYONAL CARCINOMA CELLS. J. Biol. Chem. 2003; 278: 50691-50701 © the American Society for Biochemistry and Molecular Biology
Abstract	Engelbreth-Holm-Swarm (EHS) tumors produce large amounts of basement membrane (BM) components that are widely used as cell culture substrates mimicking BM functions. To delineate the tissue/organ origin of the tumor and the mechanisms operating in the BM overproduction, a genome-wide expression profile of EHS tumor was analyzed using RIKEN cDNA microarrays containing ∼40,000 mouse cDNA clones. Expression profiles of F9 embryonal carcinoma cells that produce laminin-1 and other BM components upon differentiation into parietal endoderm-like cells (designated F9-PE) were also analyzed. Hierarchical clustering analysis showed that the gene expression profiles of EHS and F9-PE were the most similar among 49 mouse tissues/organs in the RIKEN Expression Array Database, suggesting that EHS tumor is parietal endoderm-derived. Quantitative PCR analysis confirmed that not only BM components but also the machineries required for efficient production of BM components, such as enzymes involved in post-translational modification and molecular chaperones, were highly expressed in both EHS and F9-PE. Pairs of similar transcription factor isoforms, such as Gata4/Gata6, Sox7/Sox17, and Cited1/Cited2, were also highly expressed in both EHS tumor and F9-PE. Time course analysis of F9 differentiation showed that up-regulation of the transcription factors was associated with those of BM components, suggesting their involvement in parietal endoderm specification and overproduction of the BM components.
Publisher	American Society for Biochemistry and Molecular Biology
Source Title	Journal of Biological Chemistry
Volume (Issue)	278(50)
Page	50691-50701
Date of Issued	2003-12
Language	English
Handle URL	https://hdl.handle.net/11094/71433
eISSN	1083351X
NCID	AA1202441X
Relation.isIdenticalTo	DOI (Publisher Version) https://doi.org/10.1074/jbc.M304985200
Access Rights	open access
oaire:version	Version of Record
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCMI Type	Text
DCTERMS.bibliographicCitation	Journal of Biological Chemistry. 2003, 278(50), p. 50691-50701
DC.title	Molecular Basis of Constitutive Production of Basement Membrane Components : GENE EXPRESSION PROFILES OF ENGELBRETH-HOLM-SWARM TUMOR AND F9 EMBRYONAL CARCINOMA CELLS
DC.creator	Futaki, Sugiko
	Hayashi, Yoshitaka
	Yamashita, Megumi
	Yagi, Ken
	Bono, Hidemasa
	Hayashizaki, Yoshihide
	Okazaki, Yasushi
	Sekiguchi, Kiyotoshi
DC.publisher	American Society for Biochemistry and Molecular Biology
DC.language' scheme='DCTERMS.RFC1766	English
DC.type' scheme='DCTERMS.DCMIType	Text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2003-12
DC.identifier	https://doi.org/10.1074/jbc.M304985200
DC.description	This research was originally published in the Journal of Biological Chemistry. Sugiko Futaki, Yoshitaka Hayashi, Megumi Yamashita, Ken Yagi, Hidemasa Bono, Yoshihide Hayashizaki, Yasushi Okazaki and Kiyotoshi Sekiguchi. Molecular Basis of Constitutive Production of Basement Membrane Components : GENE EXPRESSION PROFILES OF ENGELBRETH-HOLM-SWARM TUMOR AND F9 EMBRYONAL CARCINOMA CELLS. J. Biol. Chem. 2003; 278: 50691-50701 © the American Society for Biochemistry and Molecular Biology
DCTERMS.abstract	Engelbreth-Holm-Swarm (EHS) tumors produce large amounts of basement membrane (BM) components that are widely used as cell culture substrates mimicking BM functions. To delineate the tissue/organ origin of the tumor and the mechanisms operating in the BM overproduction, a genome-wide expression profile of EHS tumor was analyzed using RIKEN cDNA microarrays containing ∼40,000 mouse cDNA clones. Expression profiles of F9 embryonal carcinoma cells that produce laminin-1 and other BM components upon differentiation into parietal endoderm-like cells (designated F9-PE) were also analyzed. Hierarchical clustering analysis showed that the gene expression profiles of EHS and F9-PE were the most similar among 49 mouse tissues/organs in the RIKEN Expression Array Database, suggesting that EHS tumor is parietal endoderm-derived. Quantitative PCR analysis confirmed that not only BM components but also the machineries required for efficient production of BM components, such as enzymes involved in post-translational modification and molecular chaperones, were highly expressed in both EHS and F9-PE. Pairs of similar transcription factor isoforms, such as Gata4/Gata6, Sox7/Sox17, and Cited1/Cited2, were also highly expressed in both EHS tumor and F9-PE. Time course analysis of F9 differentiation showed that up-regulation of the transcription factors was associated with those of BM components, suggesting their involvement in parietal endoderm specification and overproduction of the BM components.
DC.format	71433
citation_title	Molecular Basis of Constitutive Production of Basement Membrane Components : GENE EXPRESSION PROFILES OF ENGELBRETH-HOLM-SWARM TUMOR AND F9 EMBRYONAL CARCINOMA CELLS
citation_author	Futaki, Sugiko
	Hayashi, Yoshitaka
	Yamashita, Megumi
	Yagi, Ken
	Bono, Hidemasa
	Hayashizaki, Yoshihide
	Okazaki, Yasushi
	Sekiguchi, Kiyotoshi
citation_publisher	American Society for Biochemistry and Molecular Biology
citation_language	English
citation_date	2003-12
citation_journal_title	Journal of Biological Chemistry
citation_volume	278
citation_issue	50
citation_firstpage	50691
citation_lastpage	50701
citation_doi	https://doi.org/10.1074/jbc.M304985200
citation_public_url	https://hdl.handle.net/11094/71433

Molecular Basis of Constitutive Production of Basement Membrane Components : GENE EXPRESSION PROFILES OF ENGELBRETH-HOLM-SWARM TUMOR AND F9 EMBRYONAL CARCINOMA CELLS

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