Identification of an Upstream Enhancer in the Mouse Lamininα1 Gene Defining Its High Level of Expression in Parietal Endoderm Cells

Niimi, Tomoaki; Hayashi, Yoshitaka; Sekiguchi, Kiyotoshi

Journal of Biological Chemistry, 2003, 278(11), 9332-9338

Number of Access:420（2025-08-18 12:36 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/71434

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Title
Identification of an Upstream Enhancer in the Mouse Lamininα1 Gene Defining Its High Level of Expression in Parietal Endoderm Cells
Creator
NRID
1000030377791 1000030377791
CreatorName
Niimi, Tomoaki
NRID
1000080420363 1000080420363
CreatorName
Hayashi, Yoshitaka
NRID
1000050187845 1000050187845
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Scopus Author ID
7201662709 7201662709
ORCID
0000-0002-6433-1410 0000-0002-6433-1410
CreatorName
Sekiguchi, Kiyotoshi
Description
This research was originally published in the Journal of Biological Chemistry. Tomoaki Niimi, Yoshitaka Hayashi and Kiyotoshi Sekiguchi. Identification of an Upstream Enhancer in the Mouse Lamininα1 Gene Defining Its High Level of Expression in Parietal Endoderm Cells. J. Biol. Chem. 2003; 278: 9332–9338 © the American Society for Biochemistry and Molecular Biology
Abstract
Laminin-1 is the major component of the embryonic basement membrane and consists of α1, β1, and γ1 chains. The expression of laminin-1 is induced in mouse F9 embryonal carcinoma cells upon differentiation into parietal endoderm through transcriptional up-regulation of the genes encoding these subunits. Here, we identified a 435-bp enhancer in the 5′-flanking region of the mouse laminin α1 (LAMA1) gene that activated its transcription in a differentiation-dependent manner. This enhancer was also active in PYS-2 parietal yolk sac-derived cells but not in NIH/3T3 fibroblasts, indicating that it was a parietal endoderm-specific enhancer. This enhancer was also active in Engelbreth-Holm-Swarm (EHS) tumor-derived cells characterized by excessive production of laminin-1 and other basement membrane components, suggesting that EHS tumors have a transcriptional control mechanism similar to that of parietal endoderm cells. Electrophoretic mobility shift analyses revealed four protein binding sites (PBS1-PBS4) in the 435-bp region. However, these DNA-binding proteins were detected not only in parietal endoderm cells (i.e. differentiated F9 cells, PYS-2 cells, and EHS tumor-derived cells) but also in undifferentiated F9 cells and NIH/3T3 cells. Mutational analyses revealed that three of these binding sites (PBS2, PBS3, and PBS4) function synergistically to confer the parietal endoderm-specific enhancer activity. The proteins binding to PBS2 and PBS4 were identified as the Sp1/Sp3 family of transcription factors and YY1, respectively.
Publisher
American Society for Biochemistry and Molecular Biology
Source Title
Journal of Biological Chemistry
Volume (Issue)
278(11)
Page
9332-9338
Date of Issued
2003-03
Language
English
Handle URL
https://hdl.handle.net/11094/71434
eISSN
1083351X
NCID
AA1202441X
Relation.isIdenticalTo
DOI (Publisher Version)
https://doi.org/10.1074/jbc.M212578200
Access Rights
open access
oaire:version
Version of Record
Category
学術雑誌論文 Journal Article