Laminin-10/11 and Fibronectin Differentially Prevent Apoptosis Induced by Serum Removal via Phosphatidylinositol 3-Kinase/Akt- and MEK1/ERK-dependent Pathways

Gu, Jianguo; Fujibayashi, Akemi; Yamada, Kenneth M. et al.

Journal of Biological Chemistry, 2002, 277(22), 19922-19928

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Identifier to cite or link to this item: https://hdl.handle.net/11094/71435

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Title
Laminin-10/11 and Fibronectin Differentially Prevent Apoptosis Induced by Serum Removal via Phosphatidylinositol 3-Kinase/Akt- and MEK1/ERK-dependent Pathways
Creator
NRID
1000040260369 1000040260369
CreatorName
Gu, Jianguo
CreatorName
Fujibayashi, Akemi
CreatorName
Yamada, Kenneth M.
NRID
1000050187845 1000050187845
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Scopus Author ID
7201662709 7201662709
ORCID
0000-0002-6433-1410 0000-0002-6433-1410
CreatorName
Sekiguchi, Kiyotoshi
Description
This research was originally published in the Journal of Biological Chemistry. Jianguo Gu, Akemi Fujibayashi, Kenneth M. Yamada and Kiyotoshi Sekiguchi. Laminin-10/11 and Fibronectin Differentially Prevent Apoptosis Induced by Serum Removal via Phosphatidylinositol 3-Kinase/Akt- and MEK1/ERK-dependent Pathways. J. Biol. Chem. 2003; 277: 19922–19928 © the American Society for Biochemistry and Molecular Biology
Abstract
Cell adhesion to the extracellular matrix inhibits apoptosis, but the molecular mechanisms underlying the signals transduced by different matrix components are not well understood. Here, we examined integrin-mediated antiapoptotic signals from laminin-10/11 in comparison with those from fibronectin, the best characterized extracellular adhesive ligand. We found that the activation of protein kinase B/Akt in cells adhering to laminin-10/11 can rescue cell apoptosis induced by serum removal. Consistent with this, wortmannin, a specific inhibitor of phosphatidylinositol 3-kinase, or ectopic expression of a dominant-negative mutant of Akt selectively accelerated cell death upon serum removal. In contrast to laminin-10/11, fibronectin rescued cells from serum depletion-induced apoptosis mainly through the extracellular signal-regulated kinase pathway. Cell survival on fibronectin but not laminin was significantly reduced by treatment with PD98059, a specific inhibitor of mitogen- or extracellular signal-regulated kinase kinase-1 (MEK1) and by expression of a dominant-negative mutant of MEK1. Laminin-10/11 was more potent than fibronectin in preventing apoptosis induced by serum depletion. These results, taken together, demonstrate laminin-10/11 potency as a survival factor and demonstrate that different extracellular matrix components can transduce distinct survival signals through preferential activation of subsets of multiple integrin-mediated signaling pathways.
Publisher
American Society for Biochemistry and Molecular Biology
Source Title
Journal of Biological Chemistry
Volume (Issue)
277(22)
Page
19922-19928
Date of Issued
2002-05
Language
English
Handle URL
https://hdl.handle.net/11094/71435
eISSN
1083351X
NCID
AA1202441X
Relation.isIdenticalTo
DOI (Publisher Version)
https://doi.org/10.1074/jbc.M200383200
Access Rights
open access
oaire:version
Version of Record
Category
学術雑誌論文 Journal Article