Deregulation of alternative splicing of fibronectin pre-mRNA in malignant human liver tumors

Oyama, Fumitaka; Hirohashi, Setsuo; Shimosato, Yukio; Titani, Koiti; Sekiguchi, Kiyotoshi

doi:https://doi.org/10.1016/S0021-9258(18)81621-0

Deregulation of alternative splicing of fibronectin pre-mRNA in malignant human liver tumors

Oyama, Fumitaka; Hirohashi, Setsuo; Shimosato, Yukio et al.

Journal of Biological Chemistry, 1989, 264(18), 10331-10334

Number of Access:482（2025-08-18 12:35 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/71444

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Title	Deregulation of alternative splicing of fibronectin pre-mRNA in malignant human liver tumors
Creator	NRID 1000040194641 1000040194641 CreatorName Oyama, Fumitaka
	NRID 1000070129625 1000070129625 CreatorName Hirohashi, Setsuo
	CreatorName Shimosato, Yukio
	NRID 1000060179942 1000060179942 CreatorName Titani, Koiti
	NRID 1000050187845 1000050187845 researchmap permalink read0054833 read0054833 Scopus Author ID 7201662709 7201662709 ORCID 0000-0002-6433-1410 0000-0002-6433-1410 CreatorName Sekiguchi, Kiyotoshi
Description	This research was originally published in the Journal of Biological Chemistry. F Oyama, S Hirohashi, Y Shimosato, K Titani and K Sekiguchi. Deregulation of alternative splicing of fibronectin pre-mRNA in malignant human liver tumors. J. Biol. Chem. 1989; 264: 10331-10334 © the American Society for Biochemistry and Molecular Biology
Abstract	Alternative splicing of fibronectin pre-mRNA at the ED-A region has been shown to be regulated in a tissue- and developmental stage-specific manner. We investigated the splicing pattern at this region in malignant and nonmalignant human liver tissues and found that the relative population of the fibronectin mRNA containing the ED-A sequence is markedly increased in malignant liver tumors. Nontumorous liver tissues including those with chronic hepatitis and cirrhosis did not show any significant change in the alternative splicing at the ED-A region. It was also found that the increased expression of the ED-A-containing fibronectin mRNA closely correlates with the occurrence of portal vein tumor thrombus and intrahepatic metastasis, which are two characteristic features of invasive liver tumors. These results indicate that tissue-specific alternative splicing of fibronectin mRNA is modified in human liver cancer and raise a possibility that the putative molecular machinery governing alternative RNA splicing of not only fibronectin but also other cellular proteins is deregulated in malignant human tumors.
Publisher	American Society for Biochemistry and Molecular Biology
Source Title	Journal of Biological Chemistry
Volume (Issue)	264(18)
Page	10331-10334
Date of Issued	1989-06
Language	English
Handle URL	https://hdl.handle.net/11094/71444
eISSN	1083351X
NCID	AA1202441X
Relation.isIdenticalTo	DOI (Publisher Version) https://doi.org/10.1016/S0021-9258(18)81621-0
Access Rights	open access
oaire:version	Version of Record
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCMI Type	Text
DCTERMS.bibliographicCitation	Journal of Biological Chemistry. 1989, 264(18), p. 10331-10334
DC.title	Deregulation of alternative splicing of fibronectin pre-mRNA in malignant human liver tumors
DC.creator	Oyama, Fumitaka
	Hirohashi, Setsuo
	Shimosato, Yukio
	Titani, Koiti
	Sekiguchi, Kiyotoshi
DC.publisher	American Society for Biochemistry and Molecular Biology
DC.language' scheme='DCTERMS.RFC1766	English
DC.type' scheme='DCTERMS.DCMIType	Text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	1989-06
DC.identifier	https://doi.org/10.1016/S0021-9258(18)81621-0
DC.description	This research was originally published in the Journal of Biological Chemistry. F Oyama, S Hirohashi, Y Shimosato, K Titani and K Sekiguchi. Deregulation of alternative splicing of fibronectin pre-mRNA in malignant human liver tumors. J. Biol. Chem. 1989; 264: 10331-10334 © the American Society for Biochemistry and Molecular Biology
DCTERMS.abstract	Alternative splicing of fibronectin pre-mRNA at the ED-A region has been shown to be regulated in a tissue- and developmental stage-specific manner. We investigated the splicing pattern at this region in malignant and nonmalignant human liver tissues and found that the relative population of the fibronectin mRNA containing the ED-A sequence is markedly increased in malignant liver tumors. Nontumorous liver tissues including those with chronic hepatitis and cirrhosis did not show any significant change in the alternative splicing at the ED-A region. It was also found that the increased expression of the ED-A-containing fibronectin mRNA closely correlates with the occurrence of portal vein tumor thrombus and intrahepatic metastasis, which are two characteristic features of invasive liver tumors. These results indicate that tissue-specific alternative splicing of fibronectin mRNA is modified in human liver cancer and raise a possibility that the putative molecular machinery governing alternative RNA splicing of not only fibronectin but also other cellular proteins is deregulated in malignant human tumors.
DC.format	71444
citation_title	Deregulation of alternative splicing of fibronectin pre-mRNA in malignant human liver tumors
citation_author	Oyama, Fumitaka
	Hirohashi, Setsuo
	Shimosato, Yukio
	Titani, Koiti
	Sekiguchi, Kiyotoshi
citation_publisher	American Society for Biochemistry and Molecular Biology
citation_language	English
citation_date	1989-06
citation_journal_title	Journal of Biological Chemistry
citation_volume	264
citation_issue	18
citation_firstpage	10331
citation_lastpage	10334
citation_doi	https://doi.org/10.1016/S0021-9258(18)81621-0
citation_public_url	https://hdl.handle.net/11094/71444

Deregulation of alternative splicing of fibronectin pre-mRNA in malignant human liver tumors

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