A monoclonal antibody directed to N-acetylneuraminosyl-alpha 2 leads to 6-galactosyl residue in gangliosides and glycoproteins

Hakomori, Sen-itiroh; Patterson, Charles M.; Nudelman, Edward et al.

Journal of Biological Chemistry, 1983, 258(19), 11819-11822

Number of Access:437（2025-08-18 12:34 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/71447

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Title
A monoclonal antibody directed to N-acetylneuraminosyl-alpha 2 leads to 6-galactosyl residue in gangliosides and glycoproteins
Creator
CreatorName
Hakomori, Sen-itiroh
CreatorName
Patterson, Charles M.
CreatorName
Nudelman, Edward
NRID
1000050187845 1000050187845
researchmap permalink
read0054833 read0054833
Scopus Author ID
7201662709 7201662709
ORCID
0000-0002-6433-1410 0000-0002-6433-1410
CreatorName
Sekiguchi, Kiyotoshi
Description
This research was originally published in the Journal of Biological Chemistry. S Hakomori, C M Patterson, E Nudelman and K Sekiguchi. A monoclonal antibody directed to N-acetylneuraminosyl-alpha 2 leads to 6-galactosyl residue in gangliosides and glycoproteins. J. Biol. Chem. 1983; 258: 11819-11822 © the American Society for Biochemistry and Molecular Biology
Abstract
A hybridoma cell line producing a monoclonal antibody directed to N-acetylneuraminosyl-alpha 2 leads to 6-galactosyl residue has been established. The antibody is IgG2b and reacts only with lacto-series gangliosides as well as with glycoproteins having an N-acetylneuraminosyl-alpha 2 leads to 6-galactosyl residue, but does not react with gangliosides or glycoproteins having an N-acetylneuraminosyl-alpha 2 leads to 3- or -alpha 2 leads to 4-galactosyl residue. The antibody is useful for detecting the specific carbohydrate chain having this terminal structure by immunostaining of glycolipids separated on thin layer chromatography or glycoproteins separated on gel electrophoresis after blotting on nitrocellulose sheet. A remarkable accumulation of a few gangliosides having this terminal structure has been detected by this monoclonal antibody in some human cancer.
Publisher
American Society for Biochemistry and Molecular Biology
Source Title
Journal of Biological Chemistry
Volume (Issue)
258(19)
Page
11819-11822
Date of Issued
1983-10
Language
English
Handle URL
https://hdl.handle.net/11094/71447
eISSN
1083351X
NCID
AA1202441X
Relation.references
URI
http://www.jbc.org/content/258/19/11819
Access Rights
open access
oaire:version
Version of Record
Category
学術雑誌論文 Journal Article