A novel efficient feeder-free culture system for the derivation of human induced pluripotent stem cells

Nakagawa, Masato; Taniguchi, Yukimasa; Senda, Sho; Takizawa, Nanako; Ichisaka, Tomoko; Asano, Kanako; Morizane, Asuka; Doi, Daisuke; Takahashi, Jun; Nishizawa, Masatoshi; Yoshida, Yoshinori; Toyoda, Taro; Osafune, Kenji; Sekiguchi, Kiyotoshi; Yamanaka, Shinya

doi:https://doi.org/10.1038/srep03594

A novel efficient feeder-free culture system for the derivation of human induced pluripotent stem cells

Nakagawa, Masato; Taniguchi, Yukimasa; Senda, Sho et al.

Scientific Reports, 2014, 4, 3594

Number of Access:1,530（2025-08-18 14:22 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/71804

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Title	A novel efficient feeder-free culture system for the derivation of human induced pluripotent stem cells
Creator	NRID 1000010379539 1000010379539 CreatorName Nakagawa, Masato
	CreatorName Taniguchi, Yukimasa
	CreatorName Senda, Sho
	CreatorName Takizawa, Nanako
	CreatorName Ichisaka, Tomoko
	CreatorName Asano, Kanako
	NRID 1000010528730 1000010528730 CreatorName Morizane, Asuka
	CreatorName Doi, Daisuke
	NRID 1000010270779 1000010270779 CreatorName Takahashi, Jun
	CreatorName Nishizawa, Masatoshi
	NRID 1000020447965 1000020447965 CreatorName Yoshida, Yoshinori
	NRID 1000060593530 1000060593530 CreatorName Toyoda, Taro
	NRID 1000080502947 1000080502947 CreatorName Osafune, Kenji
	NRID 1000050187845 1000050187845 researchmap permalink read0054833 read0054833 Scopus Author ID 7201662709 7201662709 ORCID 0000-0002-6433-1410 0000-0002-6433-1410 CreatorName Sekiguchi, Kiyotoshi
	NRID 1000010295694 1000010295694 CreatorName Yamanaka, Shinya
Description	Nakagawa, M.et al. A novel efficient feeder-free culture system forthe derivation of human induced pluripotent stem cells.Sci. Rep.4, 3594; DOI:10.1038/srep03594 (2014).
Abstract	In order to apply human embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs) to regenerative medicine, the cells should be produced under restricted conditions conforming to GMP guidelines. Since the conventional culture system has some issues that need to be addressed to achieve this goal, we developed a novel culture system. We found that recombinant laminin-511 E8 fragments are useful matrices for maintaining hESCs and hiPSCs when used in combination with a completely xeno-free (Xf) medium, StemFit™. Using this system, hESCs and hiPSCs can be easily and stably passaged by dissociating the cells into single cells for long periods, without any karyotype abnormalities. Human iPSCs could be generated under feeder-free (Ff) and Xf culture systems from human primary fibroblasts and blood cells, and they possessed differentiation abilities. These results indicate that hiPSCs can be generated and maintained under this novel Ff and Xf culture system.
Publisher	Springer Nature
Source Title	Scientific Reports
Volume (Issue)	4
Page	3594
Date of Issued	2014-01
Language	English
Handle URL	https://hdl.handle.net/11094/71804
eISSN	20452322
Relation.isIdenticalTo	DOI (Publisher Version) https://doi.org/10.1038/srep03594
Access Rights	open access
CopyRight	© 2014 The Author(s). This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License.
oaire:version	Version of Record
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCMI Type	Text
DCTERMS.bibliographicCitation	Scientific Reports. 2014, 4, p. 3594
DC.title	A novel efficient feeder-free culture system for the derivation of human induced pluripotent stem cells
DC.creator	Nakagawa, Masato
	Taniguchi, Yukimasa
	Senda, Sho
	Takizawa, Nanako
	Ichisaka, Tomoko
	Asano, Kanako
	Morizane, Asuka
	Doi, Daisuke
	Takahashi, Jun
	Nishizawa, Masatoshi
	Yoshida, Yoshinori
	Toyoda, Taro
	Osafune, Kenji
	Sekiguchi, Kiyotoshi
	Yamanaka, Shinya
DC.publisher	Springer Nature
DC.language' scheme='DCTERMS.RFC1766	English
DC.type' scheme='DCTERMS.DCMIType	Text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2014-01
DC.identifier	https://doi.org/10.1038/srep03594
DC.description	Nakagawa, M.et al. A novel efficient feeder-free culture system forthe derivation of human induced pluripotent stem cells.Sci. Rep.4, 3594; DOI:10.1038/srep03594 (2014).
DCTERMS.abstract	In order to apply human embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs) to regenerative medicine, the cells should be produced under restricted conditions conforming to GMP guidelines. Since the conventional culture system has some issues that need to be addressed to achieve this goal, we developed a novel culture system. We found that recombinant laminin-511 E8 fragments are useful matrices for maintaining hESCs and hiPSCs when used in combination with a completely xeno-free (Xf) medium, StemFit™. Using this system, hESCs and hiPSCs can be easily and stably passaged by dissociating the cells into single cells for long periods, without any karyotype abnormalities. Human iPSCs could be generated under feeder-free (Ff) and Xf culture systems from human primary fibroblasts and blood cells, and they possessed differentiation abilities. These results indicate that hiPSCs can be generated and maintained under this novel Ff and Xf culture system.
DC.rights	© 2014 The Author(s). This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License.
DC.format	71804
citation_title	A novel efficient feeder-free culture system for the derivation of human induced pluripotent stem cells
citation_author	Nakagawa, Masato
	Taniguchi, Yukimasa
	Senda, Sho
	Takizawa, Nanako
	Ichisaka, Tomoko
	Asano, Kanako
	Morizane, Asuka
	Doi, Daisuke
	Takahashi, Jun
	Nishizawa, Masatoshi
	Yoshida, Yoshinori
	Toyoda, Taro
	Osafune, Kenji
	Sekiguchi, Kiyotoshi
	Yamanaka, Shinya
citation_publisher	Springer Nature
citation_language	English
citation_date	2014-01
citation_journal_title	Scientific Reports
citation_volume	4
citation_firstpage	3594
citation_doi	https://doi.org/10.1038/srep03594
citation_public_url	https://hdl.handle.net/11094/71804

A novel efficient feeder-free culture system for the derivation of human induced pluripotent stem cells

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