Selective Laminin-Directed Differentiation of Human Induced Pluripotent Stem Cells into Distinct Ocular Lineages

Shibata, Shun; Hayashi, Ryuhei; Okubo, Toru; Kudo, Yuji; Katayama, Tomohiko; Ishikawa, Yuko; Toga, Junko; Yagi, Emiko; Honma, Yoichi; Quantock, Andrew J.; Sekiguchi, Kiyotoshi; Nishida, Kohji

doi:https://doi.org/10.1016/j.celrep.2018.10.032

Selective Laminin-Directed Differentiation of Human Induced Pluripotent Stem Cells into Distinct Ocular Lineages

Shibata, Shun; Hayashi, Ryuhei; Okubo, Toru et al.

Cell Reports, 2018, 25(6), 1668-1679

Number of Access:1,067（2025-08-18 14:33 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/71805

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Title	Selective Laminin-Directed Differentiation of Human Induced Pluripotent Stem Cells into Distinct Ocular Lineages
Creator	CreatorName Shibata, Shun
	NRID 1000070535278 1000070535278 ORCID 0000-0002-4699-8617 0000-0002-4699-8617 researchmap permalink stemed0701 stemed0701 Scopus Author ID 23012200500 23012200500 CreatorName Hayashi, Ryuhei
	CreatorName Okubo, Toru
	CreatorName Kudo, Yuji
	NRID 1000080897942 1000080897942 researchmap permalink phoeni457 phoeni457 CreatorName Katayama, Tomohiko
	CreatorName Ishikawa, Yuko
	CreatorName Toga, Junko
	CreatorName Yagi, Emiko
	CreatorName Honma, Yoichi
	CreatorName Quantock, Andrew J.
	NRID 1000050187845 1000050187845 researchmap permalink read0054833 read0054833 Scopus Author ID 7201662709 7201662709 ORCID 0000-0002-6433-1410 0000-0002-6433-1410 CreatorName Sekiguchi, Kiyotoshi
	NRID 1000040244610 1000040244610 ORCID 0000-0001-9069-3610 0000-0001-9069-3610 researchmap permalink read0131487 read0131487 Scopus Author ID 57211973404 57211973404 CreatorName Nishida, Kohji
Description	Shun Shibata, Ryuhei Hayashi, ToruOkubo. Yuji Kudo, Tomohiko Katayama, Yuko Ishikawa, Junko Toga, Emiko Yagi. Yoichi Honma, Andrew J. Quantock, Kiyotoshi Sekiguchi, Kohji Nishida, "Selective Laminin-Directed Differentiation of Human Induced Pluripotent Stem Cells into Distinct Ocular Lineages", Cell Reports, Vol. 25, Issue 6, 6 pp. 1668-1679, Elsevier, 2018
Abstract	The extracellular matrix plays a key role in stem cell maintenance, expansion, and differentiation. Laminin, a basement membrane protein, is a widely used substrate for cell culture including the growth of human induced pluripotent stem cells (hiPSCs). Here, we show that different isoforms of laminin lead to the selective differentiation of hiPSCs into different eye-like tissues. Specifically, the 211 isoform of the E8 fragment of laminin (LN211E8) promotes differentiation into neural crest cells via Wnt activation, whereas LN332E8 promotes differentiation into corneal epithelial cells. The immunohistochemical distributions of these laminin isoforms in the developing mouse eye mirrors the hiPSC type that was induced in vitro. Moreover, LN511E8 enables generation of dense hiPSC colonies due to actomyosin contraction, which in turn led to cell density-dependent YAP inactivation and subsequent retinal differentiation in colony centers. Thus, distinct laminin isoforms determine the fate of expanded hiPSCs into eye-like tissues.
Publisher	Elsevier
Source Title	Cell Reports
Volume (Issue)	25(6)
Page	1668-1679
Date of Issued	2018-11
Language	English
Handle URL	https://hdl.handle.net/11094/71805
eISSN	22111247
Relation.isIdenticalTo	DOI (Publisher Version) https://doi.org/10.1016/j.celrep.2018.10.032
Access Rights	open access
CopyRight	© 2018 The Authors. This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
oaire:version	Version of Record
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCMI Type	Text
DCTERMS.bibliographicCitation	Cell Reports. 2018, 25(6), p. 1668-1679
DC.title	Selective Laminin-Directed Differentiation of Human Induced Pluripotent Stem Cells into Distinct Ocular Lineages
DC.creator	Shibata, Shun
	Hayashi, Ryuhei
	Okubo, Toru
	Kudo, Yuji
	Katayama, Tomohiko
	Ishikawa, Yuko
	Toga, Junko
	Yagi, Emiko
	Honma, Yoichi
	Quantock, Andrew J.
	Sekiguchi, Kiyotoshi
	Nishida, Kohji
DC.publisher	Elsevier
DC.language' scheme='DCTERMS.RFC1766	English
DC.type' scheme='DCTERMS.DCMIType	Text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2018-11
DC.identifier	https://doi.org/10.1016/j.celrep.2018.10.032
DC.description	Shun Shibata, Ryuhei Hayashi, ToruOkubo. Yuji Kudo, Tomohiko Katayama, Yuko Ishikawa, Junko Toga, Emiko Yagi. Yoichi Honma, Andrew J. Quantock, Kiyotoshi Sekiguchi, Kohji Nishida, "Selective Laminin-Directed Differentiation of Human Induced Pluripotent Stem Cells into Distinct Ocular Lineages", Cell Reports, Vol. 25, Issue 6, 6 pp. 1668-1679, Elsevier, 2018
DCTERMS.abstract	The extracellular matrix plays a key role in stem cell maintenance, expansion, and differentiation. Laminin, a basement membrane protein, is a widely used substrate for cell culture including the growth of human induced pluripotent stem cells (hiPSCs). Here, we show that different isoforms of laminin lead to the selective differentiation of hiPSCs into different eye-like tissues. Specifically, the 211 isoform of the E8 fragment of laminin (LN211E8) promotes differentiation into neural crest cells via Wnt activation, whereas LN332E8 promotes differentiation into corneal epithelial cells. The immunohistochemical distributions of these laminin isoforms in the developing mouse eye mirrors the hiPSC type that was induced in vitro. Moreover, LN511E8 enables generation of dense hiPSC colonies due to actomyosin contraction, which in turn led to cell density-dependent YAP inactivation and subsequent retinal differentiation in colony centers. Thus, distinct laminin isoforms determine the fate of expanded hiPSCs into eye-like tissues.
DC.rights	© 2018 The Authors. This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
DC.format	71805
citation_title	Selective Laminin-Directed Differentiation of Human Induced Pluripotent Stem Cells into Distinct Ocular Lineages
citation_author	Shibata, Shun
	Hayashi, Ryuhei
	Okubo, Toru
	Kudo, Yuji
	Katayama, Tomohiko
	Ishikawa, Yuko
	Toga, Junko
	Yagi, Emiko
	Honma, Yoichi
	Quantock, Andrew J.
	Sekiguchi, Kiyotoshi
	Nishida, Kohji
citation_publisher	Elsevier
citation_language	English
citation_date	2018-11
citation_journal_title	Cell Reports
citation_volume	25
citation_issue	6
citation_firstpage	1668
citation_lastpage	1679
citation_doi	https://doi.org/10.1016/j.celrep.2018.10.032
citation_public_url	https://hdl.handle.net/11094/71805

Selective Laminin-Directed Differentiation of Human Induced Pluripotent Stem Cells into Distinct Ocular Lineages

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