Ventricular–subventricular zone fractones are speckled basement membranes that function as a neural stem cell niche

Sato, Yuya; Kiyozumi, Daiji; Futaki, Sugiko; Nakano, Itsuko; Shimono, Chisei; Kaneko, Naoko; Ikawa, Masahito; Okabe, Masaru; Sawamoto, Kazunobu; Sekiguchi, Kiyotoshi

doi:https://doi.org/10.1091/mbc.E18-05-0286

Ventricular–subventricular zone fractones are speckled basement membranes that function as a neural stem cell niche

Sato, Yuya; Kiyozumi, Daiji; Futaki, Sugiko et al.

Molecular Biology of the Cell, 2018, 30(1), 56-68

Number of Access:1,120（2025-08-18 16:20 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/71809

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Title	Ventricular–subventricular zone fractones are speckled basement membranes that function as a neural stem cell niche
Creator	CreatorName Sato, Yuya
	NRID 1000070452430 1000070452430 ORCID 0000-0003-3289-227X 0000-0003-3289-227X researchmap permalink dkiyozumi dkiyozumi Scopus Author ID 6506341053 6506341053 CreatorName Kiyozumi, Daiji
	NRID 1000000403014 1000000403014 CreatorName Futaki, Sugiko
	CreatorName Nakano, Itsuko
	CreatorName Shimono, Chisei
	NRID 1000020464571 1000020464571 CreatorName Kaneko, Naoko
	NRID 1000020304066 1000020304066 ORCID 0000-0001-9859-6217 0000-0001-9859-6217 researchmap permalink read0054910 read0054910 Scopus Author ID 16689054500 16689054500 CreatorName Ikawa, Masahito
	NRID 1000030089875 1000030089875 CreatorName Okabe, Masaru
	NRID 1000090282350 1000090282350 CreatorName Sawamoto, Kazunobu
	NRID 1000050187845 1000050187845 researchmap permalink read0054833 read0054833 Scopus Author ID 7201662709 7201662709 ORCID 0000-0002-6433-1410 0000-0002-6433-1410 CreatorName Sekiguchi, Kiyotoshi
Description	Yuya Sato, Daiji Kiyozumi, Sugiko Futaki, Itsuko Nakano, Chisei Shimono, Naoko Kaneko, Masahito Ikawa, Masaru Okabe, Kazunobu Sawamoto, and Kiyotoshi Sekiguchi, "Ventricular–subventricular zone fractones are speckled basement membranes that function as a neural stem cell niche", Molecular Biology of the Cell, Vol. 30, No.1, 56-68, American Society for Cell Biology, 2018
Abstract	Neural stem cells (NSCs) are retained in the adult ventricular–subventricular zone (V-SVZ), a specialized neurogenic niche with a unique cellular architecture. It currently remains unclear whether or how NSCs utilize basement membranes (BMs) in this niche. Here, we examine the molecular compositions and functions of BMs in the adult mouse V-SVZ. Whole-mount V-SVZ immunostaining revealed that fractones, which are fingerlike processes of extravascular BMs, are speckled BMs unconnected to the vasculature, and differ in their molecular composition from vascular BMs. Glial fibrillary acidic protein (GFAP)-positive astrocytes and NSCs produce and adhere to speckled BMs. Furthermore, Gfap-Cre-mediated Lamc1flox(E1605Q) knockin mice, in which integrin-binding activities of laminins are specifically nullified in GFAP-positive cells, exhibit a decreased number and size of speckled BMs and reduced in vitro neurosphere-forming activity. Our results reveal niche activities of fractones/speckled BMs for NSCs and provide molecular insights into how laminin–integrin interactions regulate NSCs in vivo.
Publisher	American Society for Cell Biology
Source Title	Molecular Biology of the Cell
Volume (Issue)	30(1)
Page	56-68
Date of Issued	2018-10
Language	English
Handle URL	https://hdl.handle.net/11094/71809
eISSN	19394586
NCID	AA12024500
Relation.isIdenticalTo	DOI (Publisher Version) https://doi.org/10.1091/mbc.E18-05-0286
Access Rights	open access
CopyRight	© 2019 Sato et al. This article is distributed by The American Society for Cell Biol-ogy under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License.
oaire:version	Version of Record
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCMI Type	Text
DCTERMS.bibliographicCitation	Molecular Biology of the Cell. 2018, 30(1), p. 56-68
DC.title	Ventricular–subventricular zone fractones are speckled basement membranes that function as a neural stem cell niche
DC.creator	Sato, Yuya
	Kiyozumi, Daiji
	Futaki, Sugiko
	Nakano, Itsuko
	Shimono, Chisei
	Kaneko, Naoko
	Ikawa, Masahito
	Okabe, Masaru
	Sawamoto, Kazunobu
	Sekiguchi, Kiyotoshi
DC.publisher	American Society for Cell Biology
DC.language' scheme='DCTERMS.RFC1766	English
DC.type' scheme='DCTERMS.DCMIType	Text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2018-10
DC.identifier	https://doi.org/10.1091/mbc.E18-05-0286
DC.description	Yuya Sato, Daiji Kiyozumi, Sugiko Futaki, Itsuko Nakano, Chisei Shimono, Naoko Kaneko, Masahito Ikawa, Masaru Okabe, Kazunobu Sawamoto, and Kiyotoshi Sekiguchi, "Ventricular–subventricular zone fractones are speckled basement membranes that function as a neural stem cell niche", Molecular Biology of the Cell, Vol. 30, No.1, 56-68, American Society for Cell Biology, 2018
DCTERMS.abstract	Neural stem cells (NSCs) are retained in the adult ventricular–subventricular zone (V-SVZ), a specialized neurogenic niche with a unique cellular architecture. It currently remains unclear whether or how NSCs utilize basement membranes (BMs) in this niche. Here, we examine the molecular compositions and functions of BMs in the adult mouse V-SVZ. Whole-mount V-SVZ immunostaining revealed that fractones, which are fingerlike processes of extravascular BMs, are speckled BMs unconnected to the vasculature, and differ in their molecular composition from vascular BMs. Glial fibrillary acidic protein (GFAP)-positive astrocytes and NSCs produce and adhere to speckled BMs. Furthermore, Gfap-Cre-mediated Lamc1flox(E1605Q) knockin mice, in which integrin-binding activities of laminins are specifically nullified in GFAP-positive cells, exhibit a decreased number and size of speckled BMs and reduced in vitro neurosphere-forming activity. Our results reveal niche activities of fractones/speckled BMs for NSCs and provide molecular insights into how laminin–integrin interactions regulate NSCs in vivo.
DC.rights	© 2019 Sato et al. This article is distributed by The American Society for Cell Biol-ogy under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License.
DC.format	71809
citation_title	Ventricular–subventricular zone fractones are speckled basement membranes that function as a neural stem cell niche
citation_author	Sato, Yuya
	Kiyozumi, Daiji
	Futaki, Sugiko
	Nakano, Itsuko
	Shimono, Chisei
	Kaneko, Naoko
	Ikawa, Masahito
	Okabe, Masaru
	Sawamoto, Kazunobu
	Sekiguchi, Kiyotoshi
citation_publisher	American Society for Cell Biology
citation_language	English
citation_date	2018-10
citation_journal_title	Molecular Biology of the Cell
citation_volume	30
citation_issue	1
citation_firstpage	56
citation_lastpage	68
citation_doi	https://doi.org/10.1091/mbc.E18-05-0286
citation_public_url	https://hdl.handle.net/11094/71809

Ventricular–subventricular zone fractones are speckled basement membranes that function as a neural stem cell niche

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