Respective optimal calcium concentrations for proliferation on type I collagen fibrils in two keratinocyte line cells, HaCaT and FEPE1L-8

Fujisaki, Hitomi; Futaki, Sugiko; Yamada, Masashi et al.

Regenerative Therapy, 2018, 8, 73-79

Number of Access:731（2025-08-18 14:27 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/71811

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Title
Respective optimal calcium concentrations for proliferation on type I collagen fibrils in two keratinocyte line cells, HaCaT and FEPE1L-8
Creator
CreatorName
Fujisaki, Hitomi
NRID
1000000403014 1000000403014
CreatorName
Futaki, Sugiko
NRID
1000090304055 1000090304055
CreatorName
Yamada, Masashi
NRID
1000050187845 1000050187845
researchmap permalink
read0054833 read0054833
Scopus Author ID
7201662709 7201662709
ORCID
0000-0002-6433-1410 0000-0002-6433-1410
CreatorName
Sekiguchi, Kiyotoshi
CreatorName
Hayashi, Toshihiko
CreatorName
Ikejima, Takashi
CreatorName
Hattori, Shunji
Subject
Type I collagen gel
Keratinocyte proliferation
Calcium concentration
Description
Hitomi Fujisaki, SugikoFutaki, Masashi Yamada, Kiyotoshi Sekiguchi, Toshihiko Hayashi, Takashi Ikejima, Shunji Hattori, "Respective optimal calcium concentrations for proliferation on type I collagen fibrils in two keratinocyte line cells, HaCaT and FEPE1L-8", Regenerative Therapy, Vol. 8, 73-79, Production and Hosting by Elsevier B.V. on behalf of the Japanese Society for Regenerative Medicine, 2018
Abstract
Keratinocyte line cells HaCaT and FEPE1L-8 are used for skin model with type I collagen fibrils (gels). For this purpose, not only differentiation but also regulation of proliferation on type I collagen gels by exogenous calcium concentration is important. When exogenous calcium concentration is low, primary keratinocyte proliferation is repressed and eventually cells are induced to apoptosis on type I collagen gels. The apoptosis induced on type I collagen gels is suppressed by increasing calcium concentration in the medium. That is, higher exogenous calcium concentration is necessary for primary keratinocyte survival on type I collagen gels than for that on dish surface culture. Meanwhile much higher exogenous calcium causes cell differentiation and inhibition of proliferation. The optimal calcium concentrations for proliferation on type I collagen gels have not been clarified in keratinocyte line cells. HaCaT cells have a unique calcium sensitivity in comparison with primary keratinocytes, whereas FEPE1L-8 cells have a similar sensitivity to primary keratinocytes. In this study, we compared the effect of calcium concentrations on proliferation of HaCaT and FEPE1L-8 cells on type I collagen gels. On type I collagen gels, both line cells required higher calcium concentrations for proliferation than on dish surface. HaCaT cells proliferated better in a wider range of calcium concentrations than FEPE1L-8 cells.
Publisher
Elsevier
Source Title
Regenerative Therapy
Volume (Issue)
8
Page
73-79
Date of Issued
2018-06
Language
English
Handle URL
https://hdl.handle.net/11094/71811
eISSN
23523204
Relation.isIdenticalTo
DOI (Publisher Version)
https://doi.org/10.1016/j.reth.2018.04.001
Access Rights
open access
CopyRight
© 2018 The Japanese Society for Regenerative Medicine. Production and hosting by Elsevier B.V. This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
oaire:version
Version of Record
Category
学術雑誌論文 Journal Article