Recapitulation of Extracellular LAMININ Environment Maintains Stemness of Satellite Cells In Vitro

Ishii, Kana; Sakurai, Hidetoshi; Suzuki, Nobuharu; Mabuchi, Yo; Sekiya, Ichiro; Sekiguchi, Kiyotoshi; Akazawa, Chihiro

doi:https://doi.org/10.1016/j.stemcr.2017.12.013

Recapitulation of Extracellular LAMININ Environment Maintains Stemness of Satellite Cells In Vitro

Ishii, Kana; Sakurai, Hidetoshi; Suzuki, Nobuharu et al.

Stem Cell Reports, 2018, 10(2), 568-582

Number of Access:455（2025-08-18 14:25 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/71812

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Title	Recapitulation of Extracellular LAMININ Environment Maintains Stemness of Satellite Cells In Vitro
Creator	CreatorName Ishii, Kana
	NRID 1000080528745 1000080528745 CreatorName Sakurai, Hidetoshi
	NRID 1000030596565 1000030596565 CreatorName Suzuki, Nobuharu
	NRID 1000050424172 1000050424172 CreatorName Mabuchi, Yo
	NRID 1000010345291 1000010345291 CreatorName Sekiya, Ichiro
	NRID 1000050187845 1000050187845 researchmap permalink read0054833 read0054833 Scopus Author ID 7201662709 7201662709 ORCID 0000-0002-6433-1410 0000-0002-6433-1410 CreatorName Sekiguchi, Kiyotoshi
	NRID 1000080291160 1000080291160 CreatorName Akazawa, Chihiro
Subject	Laminin
	muscle stem cell
	cell transplantation therapy
	regeneration
	muscle satellite cell
	LM-E8
Description	Kana Ishii, Hidetoshi Sakurai, Nobuharu Suzuki, Yo Mabuchi, Ichiro Sekiya, Kiyotoshi Sekiguchi, and Chihiro Akazawa, "Recapitulation of Extracellular LAMININ Environment Maintains Stemness ofSatellite CellsIn Vitro", Stem Cell Reports, 10 (2), 568-582, Cell Press, 2018
Abstract	Satellite cells function as precursor cells in mature skeletal muscle homeostasis and regeneration. In healthy tissue, these cells are maintained in a state of quiescence by a microenvironment formed by myofibers and basement membrane in which LAMININs (LMs) form a major component. In the present study, we evaluated the satellite cell microenvironment in vivo and found that these cells are encapsulated by LMα2–5. We sought to recapitulate this satellite cell niche in vitro by culturing satellite cells in the presence of recombinant LM-E8 fragments. We show that treatment with LM-E8 promotes proliferation of satellite cells in an undifferentiated state, through reduced phosphorylation of JNK and p38. On transplantation into injured muscle tissue, satellite cells cultured with LM-E8 promoted the regeneration of skeletal muscle. These findings represent an efficient method of culturing satellite cells for use in transplantation through the recapitulation of the satellite cell niche using recombinant LM-E8 fragments.
Publisher	Cell Press
Source Title	Stem Cell Reports
Volume (Issue)	10(2)
Page	568-582
Date of Issued	2018-01
Language	English
Handle URL	https://hdl.handle.net/11094/71812
eISSN	22136711
Relation.isIdenticalTo	DOI (Publisher Version) https://doi.org/10.1016/j.stemcr.2017.12.013
Access Rights	open access
CopyRight	© 2017 The Authors. This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
oaire:version	Version of Record
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCMI Type	Text
DCTERMS.bibliographicCitation	Stem Cell Reports. 2018, 10(2), p. 568-582
DC.title	Recapitulation of Extracellular LAMININ Environment Maintains Stemness of Satellite Cells In Vitro
DC.creator	Ishii, Kana
	Sakurai, Hidetoshi
	Suzuki, Nobuharu
	Mabuchi, Yo
	Sekiya, Ichiro
	Sekiguchi, Kiyotoshi
	Akazawa, Chihiro
DC.publisher	Cell Press
DC.language' scheme='DCTERMS.RFC1766	English
DC.type' scheme='DCTERMS.DCMIType	Text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2018-01
DC.identifier	https://doi.org/10.1016/j.stemcr.2017.12.013
DC.subject	Laminin
	muscle stem cell
	cell transplantation therapy
	regeneration
	muscle satellite cell
	LM-E8
DC.description	Kana Ishii, Hidetoshi Sakurai, Nobuharu Suzuki, Yo Mabuchi, Ichiro Sekiya, Kiyotoshi Sekiguchi, and Chihiro Akazawa, "Recapitulation of Extracellular LAMININ Environment Maintains Stemness ofSatellite CellsIn Vitro", Stem Cell Reports, 10 (2), 568-582, Cell Press, 2018
DCTERMS.abstract	Satellite cells function as precursor cells in mature skeletal muscle homeostasis and regeneration. In healthy tissue, these cells are maintained in a state of quiescence by a microenvironment formed by myofibers and basement membrane in which LAMININs (LMs) form a major component. In the present study, we evaluated the satellite cell microenvironment in vivo and found that these cells are encapsulated by LMα2–5. We sought to recapitulate this satellite cell niche in vitro by culturing satellite cells in the presence of recombinant LM-E8 fragments. We show that treatment with LM-E8 promotes proliferation of satellite cells in an undifferentiated state, through reduced phosphorylation of JNK and p38. On transplantation into injured muscle tissue, satellite cells cultured with LM-E8 promoted the regeneration of skeletal muscle. These findings represent an efficient method of culturing satellite cells for use in transplantation through the recapitulation of the satellite cell niche using recombinant LM-E8 fragments.
DC.rights	© 2017 The Authors. This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
DC.format	71812
citation_title	Recapitulation of Extracellular LAMININ Environment Maintains Stemness of Satellite Cells In Vitro
citation_author	Ishii, Kana
	Sakurai, Hidetoshi
	Suzuki, Nobuharu
	Mabuchi, Yo
	Sekiya, Ichiro
	Sekiguchi, Kiyotoshi
	Akazawa, Chihiro
citation_publisher	Cell Press
citation_language	English
citation_date	2018-01
citation_journal_title	Stem Cell Reports
citation_volume	10
citation_issue	2
citation_firstpage	568
citation_lastpage	582
citation_doi	https://doi.org/10.1016/j.stemcr.2017.12.013
citation_public_url	https://hdl.handle.net/11094/71812

Recapitulation of Extracellular LAMININ Environment Maintains Stemness of Satellite Cells In Vitro

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