Laminin-511 is an epithelial message promoting dermal papilla development and function during early hair morphogenesis

Gao, Jing; DeRouen, Mindy C.; Chen, Chih-Hsin; Nguyen, Michael; Nguyen, Ngon T.; Ido, Hiroyuki; Harada, Kenji; Sekiguchi, Kiyotoshi; Morgan, Bruce A.; Miner, Jeffery H.; Oro, Anthony E.; Marinkovich, M. Peter

doi:https://doi.org/10.1101/gad.1689908

Laminin-511 is an epithelial message promoting dermal papilla development and function during early hair morphogenesis

Gao, Jing; DeRouen, Mindy C.; Chen, Chih-Hsin et al.

Genes & Development, 2008, 22(15), 2111-2124

Number of Access:1,212（2025-08-18 14:27 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/71817

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Title	Laminin-511 is an epithelial message promoting dermal papilla development and function during early hair morphogenesis
Creator	CreatorName Gao, Jing
	CreatorName DeRouen, Mindy C.
	CreatorName Chen, Chih-Hsin
	CreatorName Nguyen, Michael
	CreatorName Nguyen, Ngon T.
	CreatorName Ido, Hiroyuki
	CreatorName Harada, Kenji
	NRID 1000050187845 1000050187845 researchmap permalink read0054833 read0054833 Scopus Author ID 7201662709 7201662709 ORCID 0000-0002-6433-1410 0000-0002-6433-1410 CreatorName Sekiguchi, Kiyotoshi
	CreatorName Morgan, Bruce A.
	CreatorName Miner, Jeffery H.
	CreatorName Oro, Anthony E.
	CreatorName Marinkovich, M. Peter
Subject	Laminin
	primary cilium
	basement membrane
	integrin
	hair
Description	Jing Gao, Mindy C. DeRouen, Chih-Hsin Chen, Michael Nguyen, Ngon T. Nguyen, Hiroyuki Ido, Kenji Harada, Kiyotoshi Sekiguchi, Bruce A. Morgan, Jeffery H. Miner, Anthony E. Oro, and M. Peter Marinkovich, "Laminin-511 is an epithelial message promoting dermal papilla development and function during early hair morphogenesis", Genes & Development, 22(15) 2111-2124, Cold Spring Harbor Laboratory, 2008
Abstract	Hair morphogenesis takes place through reciprocal epithelial and mesenchymal signaling; however, the mechanisms controlling signal exchange are poorly understood. Laminins are extracellular proteins that play critical roles in adhesion and signaling. Here we demonstrate the mechanism of how laminin-511 controls hair morphogenesis. Dermal papilla (DP) from laminin-511 mutants showed developmental defects by E16.5, including a failure to maintain expression of the key morphogen noggin. This maintenance was critical as exogenous introduction of noggin or sonic hedgehog (Shh) produced downstream from noggin was sufficient to restore hair follicle development in lama5−/− (laminin-511-null) skin. Hair development required the β1 integrin binding but not the heparin binding domain of laminin-511. Previous studies demonstrated that Shh signaling requires primary cilia, microtubule-based signaling organelles. Laminin-511 mutant DP showed decreased length and structure of primary cilia in vitro and in vivo. Laminin-511, but not laminin-111, restored primary cilia formation in lama5−/− mesenchyme and triggered noggin expression in an Shh- and PDGF-dependent manner. Inhibition of laminin-511 receptor β1 integrin disrupted DP primary cilia formation as well as hair development. These studies show that epithelial-derived laminin-511 is a critical early signal that directs ciliary function and DP maintenance as a requirement for hair follicle downgrowth.
Publisher	Cold Spring Harbor Laboratory Press
Source Title	Genes & Development
Volume (Issue)	22(15)
Page	2111-2124
Date of Issued	2008-08
Language	English
Handle URL	https://hdl.handle.net/11094/71817
PISSN	08909369
eISSN	15495477
NCID	AA10668692
NCID	AA12050792
Relation.isIdenticalTo	DOI (Publisher Version) https://doi.org/10.1101/gad.1689908
Access Rights	open access
oaire:version	Version of Record
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCMI Type	Text
DCTERMS.bibliographicCitation	Genes & Development. 2008, 22(15), p. 2111-2124
DC.title	Laminin-511 is an epithelial message promoting dermal papilla development and function during early hair morphogenesis
DC.creator	Gao, Jing
	DeRouen, Mindy C.
	Chen, Chih-Hsin
	Nguyen, Michael
	Nguyen, Ngon T.
	Ido, Hiroyuki
	Harada, Kenji
	Sekiguchi, Kiyotoshi
	Morgan, Bruce A.
	Miner, Jeffery H.
	Oro, Anthony E.
	Marinkovich, M. Peter
DC.publisher	Cold Spring Harbor Laboratory Press
DC.language' scheme='DCTERMS.RFC1766	English
DC.type' scheme='DCTERMS.DCMIType	Text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2008-08
DC.identifier	https://doi.org/10.1101/gad.1689908
DC.subject	Laminin
	primary cilium
	basement membrane
	integrin
	hair
DC.description	Jing Gao, Mindy C. DeRouen, Chih-Hsin Chen, Michael Nguyen, Ngon T. Nguyen, Hiroyuki Ido, Kenji Harada, Kiyotoshi Sekiguchi, Bruce A. Morgan, Jeffery H. Miner, Anthony E. Oro, and M. Peter Marinkovich, "Laminin-511 is an epithelial message promoting dermal papilla development and function during early hair morphogenesis", Genes & Development, 22(15) 2111-2124, Cold Spring Harbor Laboratory, 2008
DCTERMS.abstract	Hair morphogenesis takes place through reciprocal epithelial and mesenchymal signaling; however, the mechanisms controlling signal exchange are poorly understood. Laminins are extracellular proteins that play critical roles in adhesion and signaling. Here we demonstrate the mechanism of how laminin-511 controls hair morphogenesis. Dermal papilla (DP) from laminin-511 mutants showed developmental defects by E16.5, including a failure to maintain expression of the key morphogen noggin. This maintenance was critical as exogenous introduction of noggin or sonic hedgehog (Shh) produced downstream from noggin was sufficient to restore hair follicle development in lama5−/− (laminin-511-null) skin. Hair development required the β1 integrin binding but not the heparin binding domain of laminin-511. Previous studies demonstrated that Shh signaling requires primary cilia, microtubule-based signaling organelles. Laminin-511 mutant DP showed decreased length and structure of primary cilia in vitro and in vivo. Laminin-511, but not laminin-111, restored primary cilia formation in lama5−/− mesenchyme and triggered noggin expression in an Shh- and PDGF-dependent manner. Inhibition of laminin-511 receptor β1 integrin disrupted DP primary cilia formation as well as hair development. These studies show that epithelial-derived laminin-511 is a critical early signal that directs ciliary function and DP maintenance as a requirement for hair follicle downgrowth.
DC.format	71817
citation_title	Laminin-511 is an epithelial message promoting dermal papilla development and function during early hair morphogenesis
citation_author	Gao, Jing
	DeRouen, Mindy C.
	Chen, Chih-Hsin
	Nguyen, Michael
	Nguyen, Ngon T.
	Ido, Hiroyuki
	Harada, Kenji
	Sekiguchi, Kiyotoshi
	Morgan, Bruce A.
	Miner, Jeffery H.
	Oro, Anthony E.
	Marinkovich, M. Peter
citation_publisher	Cold Spring Harbor Laboratory Press
citation_language	English
citation_date	2008-08
citation_journal_title	Genes & Development
citation_volume	22
citation_issue	15
citation_firstpage	2111
citation_lastpage	2124
citation_doi	https://doi.org/10.1101/gad.1689908
citation_public_url	https://hdl.handle.net/11094/71817

Laminin-511 is an epithelial message promoting dermal papilla development and function during early hair morphogenesis

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