Overexpression of β1-chain-containing laminins in capillary basement membranes of human breast cancer and its metastases

Fujita, Manabu; Khazenzon, Natalya M; Bose, Shikha; Sekiguchi, Kiyotoshi; Sasaki, Takako; Carter, William G; Ljubimov, Alexander V; Black, Keith L; Ljubimova, Julia Y

doi:https://doi.org/10.1186/bcr1011

Overexpression of β1-chain-containing laminins in capillary basement membranes of human breast cancer and its metastases

Fujita, Manabu; Khazenzon, Natalya M; Bose, Shikha et al.

Breast Cancer Research, 2005, 4(4), R411-R421

Number of Access:934（2025-08-18 14:27 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/71824

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Title	Overexpression of β1-chain-containing laminins in capillary basement membranes of human breast cancer and its metastases
Creator	CreatorName Fujita, Manabu
	CreatorName Khazenzon, Natalya M
	CreatorName Bose, Shikha
	NRID 1000050187845 1000050187845 researchmap permalink read0054833 read0054833 Scopus Author ID 7201662709 7201662709 ORCID 0000-0002-6433-1410 0000-0002-6433-1410 CreatorName Sekiguchi, Kiyotoshi
	CreatorName Sasaki, Takako
	CreatorName Carter, William G
	CreatorName Ljubimov, Alexander V
	CreatorName Black, Keith L
	CreatorName Ljubimova, Julia Y
Subject	Laminin
	Normal Breast
	Invasive Ductal Carcinoma
	Normal Breast Tissue
	Invasive Breast Carcinoma
Description	Manabu Fujita, Natalya M Khazenzon, Shikha Bose, Kiyotoshi Sekiguchi, Takako Sasaki, William G Carter, Alexander V Ljubimov, Keith L Black and Julia Y Ljubimova, "Overexpression of β1-chain-containing laminins in capillary basement membranes of human breast cancer and its metastases", Breast Cancer Research, 7, R411-R421, BioMed Central, 2005
Abstract	Introduction: Laminins are the major components of vascular and parenchymal basement membranes. We previously documented a switch in the expression of vascular laminins containing the α4 chain from predominantly laminin-9 (α4β2γ1) to predominantly laminin-8 (α4β1γ1) during progression of human brain gliomas to high-grade glioblastoma multiforme. Here, differential expression of laminins was studied in blood vessels and ductal epithelium of the breast.
	Method: In the present study the expressions of laminin isoforms α1–α5, β1–β3, γ1, and γ2 were examined during progression of breast cancer. Forty-five clinical samples of breast tissues including normal breast, ductal carcinomas in situ, invasive ductal carcinomas, and their metastases to the brain were compared using Western blot analysis and immunohistochemistry for various chains of laminin, in particular laminin-8 and laminin-9.
	Results: Laminin α4 chain was observed in vascular basement membranes of most studied tissues, with the highest expression in metastases. At the same time, the expression of laminin β2 chain (a constituent of laminin-9) was mostly seen in normal breast and carcinomas in situ but not in invasive carcinomas or metastases. In contrast, laminin β1 chain (a constituent of laminin-8) was typically found in vessel walls of carcinomas and their metastases but not in those of normal breast. The expression of laminin-8 increased in a progression-dependent manner. A similar change was observed from laminin-11 (α5β2γ1) to laminin-10 (α5β1γ1) during breast tumor progression. Additionally, laminin-2 (α2β1γ1) appeared in vascular basement membranes of invasive carcinomas and metastases. Chains of laminin-5 (α3β3γ2) were expressed in the ductal epithelium basement membranes of the breast and diminished with tumor progression.
	Conclusion: These results suggest that laminin-2, laminin-8, and laminin-10 are important components of tumor microvessels and may associate with breast tumor progression. Angiogenic switch from laminin-9 and laminin-11 to laminin-8 and laminin-10 first occurs in carcinomas in situ and becomes more pronounced with progression of carcinomas to the invasive stage. Similar to high-grade brain gliomas, the expression of laminin-8 (and laminin-10) in breast cancer tissue may be a predictive factor for tumor neovascularization and invasion.
Publisher	BioMed Central
Source Title	Breast Cancer Research
Volume (Issue)	4(4)
Page	R411-R421
Date of Issued	2005-06
Language	English
Handle URL	https://hdl.handle.net/11094/71824
eISSN	1465542X
NCID	AA12050395
Relation.isIdenticalTo	DOI (Publisher Version) https://doi.org/10.1186/bcr1011
Access Rights	open access
CopyRight	© 2005 Fujita et al.; licensee BioMed Central Ltd. This article is licensed under a Creative Commons Attribution 2.0 Generic License.
oaire:version	Version of Record
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCMI Type	Text
DCTERMS.bibliographicCitation	Breast Cancer Research. 2005, 4(4), p. R411-R421
DC.title	Overexpression of β1-chain-containing laminins in capillary basement membranes of human breast cancer and its metastases
DC.creator	Fujita, Manabu
	Khazenzon, Natalya M
	Bose, Shikha
	Sekiguchi, Kiyotoshi
	Sasaki, Takako
	Carter, William G
	Ljubimov, Alexander V
	Black, Keith L
	Ljubimova, Julia Y
DC.publisher	BioMed Central
DC.language' scheme='DCTERMS.RFC1766	English
DC.type' scheme='DCTERMS.DCMIType	Text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2005-06
DC.identifier	https://doi.org/10.1186/bcr1011
DC.subject	Laminin
	Normal Breast
	Invasive Ductal Carcinoma
	Normal Breast Tissue
	Invasive Breast Carcinoma
DC.description	Manabu Fujita, Natalya M Khazenzon, Shikha Bose, Kiyotoshi Sekiguchi, Takako Sasaki, William G Carter, Alexander V Ljubimov, Keith L Black and Julia Y Ljubimova, "Overexpression of β1-chain-containing laminins in capillary basement membranes of human breast cancer and its metastases", Breast Cancer Research, 7, R411-R421, BioMed Central, 2005
DCTERMS.abstract	Introduction: Laminins are the major components of vascular and parenchymal basement membranes. We previously documented a switch in the expression of vascular laminins containing the α4 chain from predominantly laminin-9 (α4β2γ1) to predominantly laminin-8 (α4β1γ1) during progression of human brain gliomas to high-grade glioblastoma multiforme. Here, differential expression of laminins was studied in blood vessels and ductal epithelium of the breast.
	Method: In the present study the expressions of laminin isoforms α1–α5, β1–β3, γ1, and γ2 were examined during progression of breast cancer. Forty-five clinical samples of breast tissues including normal breast, ductal carcinomas in situ, invasive ductal carcinomas, and their metastases to the brain were compared using Western blot analysis and immunohistochemistry for various chains of laminin, in particular laminin-8 and laminin-9.
	Results: Laminin α4 chain was observed in vascular basement membranes of most studied tissues, with the highest expression in metastases. At the same time, the expression of laminin β2 chain (a constituent of laminin-9) was mostly seen in normal breast and carcinomas in situ but not in invasive carcinomas or metastases. In contrast, laminin β1 chain (a constituent of laminin-8) was typically found in vessel walls of carcinomas and their metastases but not in those of normal breast. The expression of laminin-8 increased in a progression-dependent manner. A similar change was observed from laminin-11 (α5β2γ1) to laminin-10 (α5β1γ1) during breast tumor progression. Additionally, laminin-2 (α2β1γ1) appeared in vascular basement membranes of invasive carcinomas and metastases. Chains of laminin-5 (α3β3γ2) were expressed in the ductal epithelium basement membranes of the breast and diminished with tumor progression.
	Conclusion: These results suggest that laminin-2, laminin-8, and laminin-10 are important components of tumor microvessels and may associate with breast tumor progression. Angiogenic switch from laminin-9 and laminin-11 to laminin-8 and laminin-10 first occurs in carcinomas in situ and becomes more pronounced with progression of carcinomas to the invasive stage. Similar to high-grade brain gliomas, the expression of laminin-8 (and laminin-10) in breast cancer tissue may be a predictive factor for tumor neovascularization and invasion.
DC.rights	© 2005 Fujita et al.; licensee BioMed Central Ltd. This article is licensed under a Creative Commons Attribution 2.0 Generic License.
DC.format	71824
citation_title	Overexpression of β1-chain-containing laminins in capillary basement membranes of human breast cancer and its metastases
citation_author	Fujita, Manabu
	Khazenzon, Natalya M
	Bose, Shikha
	Sekiguchi, Kiyotoshi
	Sasaki, Takako
	Carter, William G
	Ljubimov, Alexander V
	Black, Keith L
	Ljubimova, Julia Y
citation_publisher	BioMed Central
citation_language	English
citation_date	2005-06
citation_journal_title	Breast Cancer Research
citation_volume	4
citation_issue	4
citation_firstpage	R411
citation_lastpage	R421
citation_doi	https://doi.org/10.1186/bcr1011
citation_public_url	https://hdl.handle.net/11094/71824

Overexpression of β1-chain-containing laminins in capillary basement membranes of human breast cancer and its metastases

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