Crystal structure of 1-aminocyclopropane-1-carboxylate deaminase from Hansenula saturnus

Yao, Min; Ose, T.; Sugimoto, Hiroshi; Horiuchi, Atsushi; Nakagawa, Atushi; Wakatsuki, Soichi; Yokoi, Daisuke; Murakami, Toyotaka; Honma, Mamoru; Tanaka, Isao

doi:https://doi.org/10.1074/jbc.M004681200

Crystal structure of 1-aminocyclopropane-1-carboxylate deaminase from Hansenula saturnus

Yao, Min; Ose, T.; Sugimoto, Hiroshi et al.

Journal of Biological Chemistry, 2000, 275(44), 34557-34565

Number of Access:740（2025-08-14 15:06 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/73646

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Title	Crystal structure of 1-aminocyclopropane-1-carboxylate deaminase from Hansenula saturnus
Creator	NRID 1000040311518 1000040311518 CreatorName Yao, Min
	CreatorName Ose, T.
	NRID 1000090344043 1000090344043 CreatorName Sugimoto, Hiroshi
	CreatorName Horiuchi, Atsushi
	NRID 1000020188890 1000020188890 ORCID 0000-0002-1700-7861 0000-0002-1700-7861 researchmap permalink atsushi_nakagawa atsushi_nakagawa Scopus Author ID 7202934909 7202934909 CreatorName Nakagawa, Atushi
	CreatorName Wakatsuki, Soichi
	CreatorName Yokoi, Daisuke
	CreatorName Murakami, Toyotaka
	NRID 1000060001442 1000060001442 CreatorName Honma, Mamoru
	NRID 1000070093052 1000070093052 CreatorName Tanaka, Isao
Description	This research was originally published in Journal of Biological Chemistry. Min Yao, T. Ose, Hiroshi Sugimoto, Atsushi Horiuchi, Atsushi Nakagawa, Soichi Wakatsuki, Daisuke Yokoii, Toyotaka Murakamii, Mamoru Honmai, and Isao Tanaka. Crystal structure of 1-aminocyclopropane-1-carboxylate deaminase from Hansenula saturnus. J. Biol. Chem. 2000; 275, 34557-34565. © the American Society for Biochemistry and Molecular Biology.
Abstract	The pyridoxal 5´-phosphate (PLP)-dependent enzyme 1-aminocyclopropane-1-carboxylate deaminase (ACCD) catalyzes a reaction that involves a ring opening of cyclopropanoid amino acid, yielding a-ketobutyrate and ammonia. Unlike other PLP-dependent enzymes, this enzyme has no a-hydrogen atom in the substrate. Thus, a unique mechanism for the bond cleavage is expected. The crystal structure of ACCD from Hansenula saturnus has been determined at 2.0 Å resolution by the multiple wavelength anomalous diffraction method using mercury atoms as anomalous scatterers. The model was built on the electron density map, which was obtained by the density averaging of multiple crystal forms. The final model was refined to an R-factor of 22.5% and an Rfree-factor of 26.8%. The ACCD folds into two domains, each of which has an open twisted a/b structure similar to the β-subunit of tryptophan synthase. However, in ACCD, unlike in other members of the β family of PLP dependent enzymes, PLP is buried deep in the molecule. The structure provides the first view of the catalytic center of the cyclopropane ring opening.
Publisher	American Society for Biochemistry and Molecular Biology
Source Title	Journal of Biological Chemistry
Volume (Issue)	275(44)
Page	34557-34565
Date of Issued	2000-11
Language	English
Handle URL	https://hdl.handle.net/11094/73646
eISSN	1083351X
NCID	AA1202441X
Relation.isIdenticalTo	DOI (Publisher Version) https://doi.org/10.1074/jbc.M004681200
Relation.isIdenticalTo	PMID 10938279
Access Rights	open access
CopyRight	© the American Society for Biochemistry and Molecular Biology.
oaire:version	Version of Record
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCMI Type	Text
DCTERMS.bibliographicCitation	Journal of Biological Chemistry. 2000, 275(44), p. 34557-34565
DC.title	Crystal structure of 1-aminocyclopropane-1-carboxylate deaminase from Hansenula saturnus
DC.creator	Yao, Min
	Ose, T.
	Sugimoto, Hiroshi
	Horiuchi, Atsushi
	Nakagawa, Atushi
	Wakatsuki, Soichi
	Yokoi, Daisuke
	Murakami, Toyotaka
	Honma, Mamoru
	Tanaka, Isao
DC.publisher	American Society for Biochemistry and Molecular Biology
DC.language' scheme='DCTERMS.RFC1766	English
DC.type' scheme='DCTERMS.DCMIType	Text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2000-11
DC.identifier	https://doi.org/10.1074/jbc.M004681200
DC.description	This research was originally published in Journal of Biological Chemistry. Min Yao, T. Ose, Hiroshi Sugimoto, Atsushi Horiuchi, Atsushi Nakagawa, Soichi Wakatsuki, Daisuke Yokoii, Toyotaka Murakamii, Mamoru Honmai, and Isao Tanaka. Crystal structure of 1-aminocyclopropane-1-carboxylate deaminase from Hansenula saturnus. J. Biol. Chem. 2000; 275, 34557-34565. © the American Society for Biochemistry and Molecular Biology.
DCTERMS.abstract	The pyridoxal 5´-phosphate (PLP)-dependent enzyme 1-aminocyclopropane-1-carboxylate deaminase (ACCD) catalyzes a reaction that involves a ring opening of cyclopropanoid amino acid, yielding a-ketobutyrate and ammonia. Unlike other PLP-dependent enzymes, this enzyme has no a-hydrogen atom in the substrate. Thus, a unique mechanism for the bond cleavage is expected. The crystal structure of ACCD from Hansenula saturnus has been determined at 2.0 Å resolution by the multiple wavelength anomalous diffraction method using mercury atoms as anomalous scatterers. The model was built on the electron density map, which was obtained by the density averaging of multiple crystal forms. The final model was refined to an R-factor of 22.5% and an Rfree-factor of 26.8%. The ACCD folds into two domains, each of which has an open twisted a/b structure similar to the β-subunit of tryptophan synthase. However, in ACCD, unlike in other members of the β family of PLP dependent enzymes, PLP is buried deep in the molecule. The structure provides the first view of the catalytic center of the cyclopropane ring opening.
DC.rights	© the American Society for Biochemistry and Molecular Biology.
DC.format	73646
citation_title	Crystal structure of 1-aminocyclopropane-1-carboxylate deaminase from Hansenula saturnus
citation_author	Yao, Min
	Ose, T.
	Sugimoto, Hiroshi
	Horiuchi, Atsushi
	Nakagawa, Atushi
	Wakatsuki, Soichi
	Yokoi, Daisuke
	Murakami, Toyotaka
	Honma, Mamoru
	Tanaka, Isao
citation_publisher	American Society for Biochemistry and Molecular Biology
citation_language	English
citation_date	2000-11
citation_journal_title	Journal of Biological Chemistry
citation_volume	275
citation_issue	44
citation_firstpage	34557
citation_lastpage	34565
citation_doi	https://doi.org/10.1074/jbc.M004681200
citation_pmid	10938279
citation_public_url	https://hdl.handle.net/11094/73646

Crystal structure of 1-aminocyclopropane-1-carboxylate deaminase from Hansenula saturnus

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