Crystal structure of the hemolytic lectin CEL-III isolated from the marine invertebrate Cucumaria echinata : Implications of domain structure for its membrane pore-formation mechanism

Uchida, Tatsuya; Yamasaki, Takayuki; Eto, Seiichiro; Sugawara, Hajime; Kurisu, Genji; Nakagawa, Atushi; Kusunoki, Masami; Hatakeyama, Tomomitsu

doi:https://doi.org/10.1074/jbc.M404065200

Crystal structure of the hemolytic lectin CEL-III isolated from the marine invertebrate Cucumaria echinata : Implications of domain structure for its membrane pore-formation mechanism

Uchida, Tatsuya; Yamasaki, Takayuki; Eto, Seiichiro et al.

Journal of Biological Chemistry, 2004, 279(35), 37133-37141

Number of Access:1,071（2025-08-14 12:35 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/73650

Link to primary information

File	Format	Terms of use	Size	Views	Date.Available	Description	information
JBC279_35_37133	pdf	None	1.14 MB	188	2019.12.06

Item Information

Output File Export EndNote Basic Export Mendeley

Title	Crystal structure of the hemolytic lectin CEL-III isolated from the marine invertebrate Cucumaria echinata : Implications of domain structure for its membrane pore-formation mechanism
Creator	NRID 1000050380564 1000050380564 CreatorName Uchida, Tatsuya
	CreatorName Yamasaki, Takayuki
	CreatorName Eto, Seiichiro
	CreatorName Sugawara, Hajime
	NRID 1000090294131 1000090294131 ORCID 0000-0002-5354-0807 0000-0002-5354-0807 researchmap permalink read0051718 read0051718 Scopus Author ID 6701675123 6701675123 CreatorName Kurisu, Genji
	NRID 1000020188890 1000020188890 ORCID 0000-0002-1700-7861 0000-0002-1700-7861 researchmap permalink atsushi_nakagawa atsushi_nakagawa Scopus Author ID 7202934909 7202934909 CreatorName Nakagawa, Atushi
	NRID 1000090135749 1000090135749 CreatorName Kusunoki, Masami
	NRID 1000050228467 1000050228467 CreatorName Hatakeyama, Tomomitsu
Description	This research was originally published in Journal of Biological Chemistry. Tatsuya Uchida, Takayuki Yamasaki, Seiichiro Eto, Hajime Sugawara, Genji Kurisu, Atsushi Nakagawa, Masami Kusunoki and Tomomitsu Hatakeyama. Crystal structure of the hemolytic lectin CEL-III isolated from the marine invertebrate Cucumaria echinata : Implications of domain structure for its membrane pore-formation mechanism. Journal of Biological Chemistry. 2004; 279, 37133-37141. © the American Society for Biochemistry and Molecular Biology.
Abstract	CEL-III is a Ca^<2＋>＋ -dependent and galactose-specific lectin purified from the sea cucumber, Cucumaria echinata, which exhibits hemolytic and hemagglutinating activities. Six molecules of CEL-III are assumed to oligomerize to form an ion-permeable pore in the cell membrane. We have determined the crystal structure of CEL-III by using single isomorphous replacement aided by anomalous scattering in lead at 1.7 Å resolution. CEL-III consists of three distinct domains as follows: the N-terminal two carbohydrate-binding domains (1 and 2), which adopt β-trefoil folds such as the B-chain of ricin and are members of the (QXW)_3 motif family; and domain 3, which is a novel fold composed of two α-helices and one β-sandwich. CEL-III is the first Ca^<2＋> -dependent lectin structure with two β-trefoil folds. Despite sharing the structure of the B-chain of ricin, CEL-III binds five Ca^<2＋> ions at five of the six subdomains in both domains 1 and 2. Considering the relatively high similarity among the five subdomains, they are putative binding sites for galactose-related carbohydrates, although it remains to be elucidated whether bound Ca^<2＋> is directly involved in interaction with carbohydrates. The paucity of hydrophobic interactions in the interfaces between the domains and biochemical data suggest that these domains rearrange upon carbohydrate binding in the erythrocyte membrane. This conformational change may be responsible for oligomerization of CEL-III molecules and hemolysis in the erythrocyte membranes.
Publisher	American Society for Biochemistry and Molecular Biology
Source Title	Journal of Biological Chemistry
Volume (Issue)	279(35)
Page	37133-37141
Date of Issued	2004-08
Language	English
Handle URL	https://hdl.handle.net/11094/73650
eISSN	1083351X
NCID	AA1202441X
Relation.isIdenticalTo	DOI (Publisher Version) https://doi.org/10.1074/jbc.M404065200
Relation.isIdenticalTo	PMID 15194688
Access Rights	open access
CopyRight	© the American Society for Biochemistry and Molecular Biology.
oaire:version	Version of Record
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCMI Type	Text
DCTERMS.bibliographicCitation	Journal of Biological Chemistry. 2004, 279(35), p. 37133-37141
DC.title	Crystal structure of the hemolytic lectin CEL-III isolated from the marine invertebrate Cucumaria echinata : Implications of domain structure for its membrane pore-formation mechanism
DC.creator	Uchida, Tatsuya
	Yamasaki, Takayuki
	Eto, Seiichiro
	Sugawara, Hajime
	Kurisu, Genji
	Nakagawa, Atushi
	Kusunoki, Masami
	Hatakeyama, Tomomitsu
DC.publisher	American Society for Biochemistry and Molecular Biology
DC.language' scheme='DCTERMS.RFC1766	English
DC.type' scheme='DCTERMS.DCMIType	Text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2004-08
DC.identifier	https://doi.org/10.1074/jbc.M404065200
DC.description	This research was originally published in Journal of Biological Chemistry. Tatsuya Uchida, Takayuki Yamasaki, Seiichiro Eto, Hajime Sugawara, Genji Kurisu, Atsushi Nakagawa, Masami Kusunoki and Tomomitsu Hatakeyama. Crystal structure of the hemolytic lectin CEL-III isolated from the marine invertebrate Cucumaria echinata : Implications of domain structure for its membrane pore-formation mechanism. Journal of Biological Chemistry. 2004; 279, 37133-37141. © the American Society for Biochemistry and Molecular Biology.
DCTERMS.abstract	CEL-III is a Ca^<2＋>＋ -dependent and galactose-specific lectin purified from the sea cucumber, Cucumaria echinata, which exhibits hemolytic and hemagglutinating activities. Six molecules of CEL-III are assumed to oligomerize to form an ion-permeable pore in the cell membrane. We have determined the crystal structure of CEL-III by using single isomorphous replacement aided by anomalous scattering in lead at 1.7 Å resolution. CEL-III consists of three distinct domains as follows: the N-terminal two carbohydrate-binding domains (1 and 2), which adopt β-trefoil folds such as the B-chain of ricin and are members of the (QXW)_3 motif family; and domain 3, which is a novel fold composed of two α-helices and one β-sandwich. CEL-III is the first Ca^<2＋> -dependent lectin structure with two β-trefoil folds. Despite sharing the structure of the B-chain of ricin, CEL-III binds five Ca^<2＋> ions at five of the six subdomains in both domains 1 and 2. Considering the relatively high similarity among the five subdomains, they are putative binding sites for galactose-related carbohydrates, although it remains to be elucidated whether bound Ca^<2＋> is directly involved in interaction with carbohydrates. The paucity of hydrophobic interactions in the interfaces between the domains and biochemical data suggest that these domains rearrange upon carbohydrate binding in the erythrocyte membrane. This conformational change may be responsible for oligomerization of CEL-III molecules and hemolysis in the erythrocyte membranes.
DC.rights	© the American Society for Biochemistry and Molecular Biology.
DC.format	73650
citation_title	Crystal structure of the hemolytic lectin CEL-III isolated from the marine invertebrate Cucumaria echinata : Implications of domain structure for its membrane pore-formation mechanism
citation_author	Uchida, Tatsuya
	Yamasaki, Takayuki
	Eto, Seiichiro
	Sugawara, Hajime
	Kurisu, Genji
	Nakagawa, Atushi
	Kusunoki, Masami
	Hatakeyama, Tomomitsu
citation_publisher	American Society for Biochemistry and Molecular Biology
citation_language	English
citation_date	2004-08
citation_journal_title	Journal of Biological Chemistry
citation_volume	279
citation_issue	35
citation_firstpage	37133
citation_lastpage	37141
citation_doi	https://doi.org/10.1074/jbc.M404065200
citation_pmid	15194688
citation_public_url	https://hdl.handle.net/11094/73650

Crystal structure of the hemolytic lectin CEL-III isolated from the marine invertebrate Cucumaria echinata : Implications of domain structure for its membrane pore-formation mechanism

Link to primary information

Item Information

Show details