Structural insights into the electron/proton transfer pathways in the quinol : fumarate reductase from Desulfovibrio gigas

Guan, Hong-Hsiang; Hsieh, Yin-Cheng; Lin, Pei-Ju; Huang, Yen-Chieh; Yoshimura, Masato; Chen, Li-Ying; Chen, Shao-Kang; Chuankhayan, Phimonphan; Lin, Chien-Chih; Chen, Nai-Chi; Nakagawa, Atushi; Chan, Sunny I.; Chen, Chun-Jung

doi:https://doi.org/10.1038/s41598-018-33193-5

Structural insights into the electron/proton transfer pathways in the quinol : fumarate reductase from Desulfovibrio gigas

Guan, Hong-Hsiang; Hsieh, Yin-Cheng; Lin, Pei-Ju et al.

Scientific Reports, 2018, 8, 33193

Number of Access:1,299（2025-08-15 06:46 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/73668

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Title	Structural insights into the electron/proton transfer pathways in the quinol : fumarate reductase from Desulfovibrio gigas
Creator	CreatorName Guan, Hong-Hsiang
	CreatorName Hsieh, Yin-Cheng
	CreatorName Lin, Pei-Ju
	CreatorName Huang, Yen-Chieh
	CreatorName Yoshimura, Masato
	CreatorName Chen, Li-Ying
	CreatorName Chen, Shao-Kang
	CreatorName Chuankhayan, Phimonphan
	CreatorName Lin, Chien-Chih
	CreatorName Chen, Nai-Chi
	NRID 1000020188890 1000020188890 ORCID 0000-0002-1700-7861 0000-0002-1700-7861 researchmap permalink atsushi_nakagawa atsushi_nakagawa Scopus Author ID 7202934909 7202934909 CreatorName Nakagawa, Atushi
	CreatorName Chan, Sunny I.
	CreatorName Chen, Chun-Jung
Description	Guan, H., Hsieh, Y., Lin, P. et al. Structural insights into the electron/proton transfer pathways in the quinol : fumarate reductase from Desulfovibrio gigas. Sci Rep 8, 14935 (2018) doi:10.1038/s41598-018-33193-5
Abstract	The membrane-embedded quinol: fumarate reductase (QFR) in anaerobic bacteria catalyzes the reduction of fumarate to succinate by quinol in the anaerobic respiratory chain. The electron/protontransfer pathways in QFRs remain controversial. Here we report the crystal structure of QFR from the anaerobic sulphate-reducing bacterium Desulfovibrio gigas (D. gigas) at 3.6 Å resolution. The structure of the D. gigas QFR is a homo-dimer, each protomer comprising two hydrophilic subunits, A and B, and one transmembrane subunit C, together with six redox cofactors including two b-hemes. One menaquinone molecule is bound near heme bL in the hydrophobic subunit C. This location of the menaquinone-binding site differs from the menaquinol-binding cavity proposed previously for QFR from Wolinella succinogenes. The observed bound menaquinone might serve as an additional redox cofactor to mediate the proton-coupled electron transport across the membrane. Armed with these structuralinsights, we propose electron/proton-transfer pathways in the quinol reduction of fumarate to succinate in the D. gigas QFR.
Publisher	Springer Nature
Source Title	Scientific Reports
Volume (Issue)	8
Page	33193
Date of Issued	2018-10
Language	English
Handle URL	https://hdl.handle.net/11094/73668
eISSN	20452322
Relation.isIdenticalTo	DOI (Publisher Version) https://doi.org/10.1038/s41598-018-33193-5
Relation.isIdenticalTo	PMID 30297797
Access Rights	open access
CopyRight	© 2018 The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License.
oaire:version	Version of Record
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCMI Type	Text
DCTERMS.bibliographicCitation	Scientific Reports. 2018, 8, p. 33193
DC.title	Structural insights into the electron/proton transfer pathways in the quinol : fumarate reductase from Desulfovibrio gigas
DC.creator	Guan, Hong-Hsiang
	Hsieh, Yin-Cheng
	Lin, Pei-Ju
	Huang, Yen-Chieh
	Yoshimura, Masato
	Chen, Li-Ying
	Chen, Shao-Kang
	Chuankhayan, Phimonphan
	Lin, Chien-Chih
	Chen, Nai-Chi
	Nakagawa, Atushi
	Chan, Sunny I.
	Chen, Chun-Jung
DC.publisher	Springer Nature
DC.language' scheme='DCTERMS.RFC1766	English
DC.type' scheme='DCTERMS.DCMIType	Text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2018-10
DC.identifier	https://doi.org/10.1038/s41598-018-33193-5
DC.description	Guan, H., Hsieh, Y., Lin, P. et al. Structural insights into the electron/proton transfer pathways in the quinol : fumarate reductase from Desulfovibrio gigas. Sci Rep 8, 14935 (2018) doi:10.1038/s41598-018-33193-5
DCTERMS.abstract	The membrane-embedded quinol: fumarate reductase (QFR) in anaerobic bacteria catalyzes the reduction of fumarate to succinate by quinol in the anaerobic respiratory chain. The electron/protontransfer pathways in QFRs remain controversial. Here we report the crystal structure of QFR from the anaerobic sulphate-reducing bacterium Desulfovibrio gigas (D. gigas) at 3.6 Å resolution. The structure of the D. gigas QFR is a homo-dimer, each protomer comprising two hydrophilic subunits, A and B, and one transmembrane subunit C, together with six redox cofactors including two b-hemes. One menaquinone molecule is bound near heme bL in the hydrophobic subunit C. This location of the menaquinone-binding site differs from the menaquinol-binding cavity proposed previously for QFR from Wolinella succinogenes. The observed bound menaquinone might serve as an additional redox cofactor to mediate the proton-coupled electron transport across the membrane. Armed with these structuralinsights, we propose electron/proton-transfer pathways in the quinol reduction of fumarate to succinate in the D. gigas QFR.
DC.rights	© 2018 The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License.
DC.format	73668
citation_title	Structural insights into the electron/proton transfer pathways in the quinol : fumarate reductase from Desulfovibrio gigas
citation_author	Guan, Hong-Hsiang
	Hsieh, Yin-Cheng
	Lin, Pei-Ju
	Huang, Yen-Chieh
	Yoshimura, Masato
	Chen, Li-Ying
	Chen, Shao-Kang
	Chuankhayan, Phimonphan
	Lin, Chien-Chih
	Chen, Nai-Chi
	Nakagawa, Atushi
	Chan, Sunny I.
	Chen, Chun-Jung
citation_publisher	Springer Nature
citation_language	English
citation_date	2018-10
citation_journal_title	Scientific Reports
citation_volume	8
citation_firstpage	33193
citation_doi	https://doi.org/10.1038/s41598-018-33193-5
citation_pmid	30297797
citation_public_url	https://hdl.handle.net/11094/73668

Structural insights into the electron/proton transfer pathways in the quinol : fumarate reductase from Desulfovibrio gigas

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