A tissue engineering approach for prenatal closure of myelomeningocele with gelatin sponges incorporating basic fibroblast growth factor

Watanabe, Miho; Jo, Jun-ichiro; Radu, Antonetta; Kaneko, Michio; Tabata, Yasuhiko; Flake, Alan W.

doi:https://doi.org/10.1089/ten.tea.2009.0532

A tissue engineering approach for prenatal closure of myelomeningocele with gelatin sponges incorporating basic fibroblast growth factor

Watanabe, Miho; Jo, Jun-ichiro; Radu, Antonetta et al.

Tissue Engineering - Part A, 2010, 16(5), 1645-1655

Number of Access:575（2025-08-15 01:09 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/78333

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Title	A tissue engineering approach for prenatal closure of myelomeningocele with gelatin sponges incorporating basic fibroblast growth factor
Creator	NRID 1000040791728 1000040791728 researchmap permalink miho miho Scopus Author ID 56282966800 56282966800 ORCID 0000-0001-6691-0611 0000-0001-6691-0611 CreatorName Watanabe, Miho
	NRID 1000060511243 1000060511243 CreatorName Jo, Jun-ichiro
	CreatorName Radu, Antonetta
	NRID 1000060152807 1000060152807 CreatorName Kaneko, Michio
	NRID 1000050211371 1000050211371 CreatorName Tabata, Yasuhiko
	CreatorName Flake, Alan W.
Description	Miho Watanabe, Jun-ichiro Jo, Antonetta Radu, Michio Kaneko, Yasuhiko Tabata, and Alan W. Flake. Tissue Engineering Part A.May 2010.1645-1655. http://doi.org/10.1089/ten.tea.2009.0532.
Abstract	Myelomeningocele (MMC) is a common and devastating malformation. Although fetal surgical closure may improve outcome, a less invasive approach that can be applied earlier in gestation is desirable. The objective of this study was to evaluate the therapeutic feasibility of a tissue engineering approach for prenatal coverage of MMC. A gelatin hydrogel composite combining a gelatin sheet and gelatin sponge was prepared with or without basic fibroblast growth factor incorporation, and applied prenatally to retinoic-acid-induced fetal MMC in the rat model. Most of the composites were adherent to the MMC within the amniotic fluid environment with the help of cyanoacrylate adhesive. Histological examination revealed cells layered over the composites with associated extracellular matrix as well as cellular ingrowth into the sponges. The layer over the composite was composed of mixed nonepithelial and epithelial cells with the extracellular matrix consisting of collagen type I and hyaluronic acid. The tissue inside the sponge consisted of nonepithelial cells and hyaluronic acid. Epidermal ingrowth underneath the sponges and neovascularization into the sponges occurred and were significantly increased by the incorporation of basic fibroblast growth factor. Although further development is needed, this study supports the therapeutic potential of a tissue engineering approach for prenatal coverage of MMC. © 2010 Mary Ann Liebert, Inc.
Publisher	Mary Ann Liebert Inc.
Source Title	Tissue Engineering - Part A
Volume (Issue)	16(5)
Page	1645-1655
Date of Issued	2010-05
Language	English
Handle URL	https://hdl.handle.net/11094/78333
PISSN	19373341
eISSN	1937335X
NCID	AA12306003
Relation.isVersionOf	DOI (Publisher Version) https://doi.org/10.1089/ten.tea.2009.0532
Relation.isIdenticalTo	PMID 19954327
Access Rights	open access
oaire:version	Accepted Manuscript
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCMI Type	Text
DCTERMS.bibliographicCitation	Tissue Engineering - Part A. 2010, 16(5), p. 1645-1655
DC.title	A tissue engineering approach for prenatal closure of myelomeningocele with gelatin sponges incorporating basic fibroblast growth factor
DC.creator	Watanabe, Miho
	Jo, Jun-ichiro
	Radu, Antonetta
	Kaneko, Michio
	Tabata, Yasuhiko
	Flake, Alan W.
DC.publisher	Mary Ann Liebert Inc.
DC.language' scheme='DCTERMS.RFC1766	English
DC.type' scheme='DCTERMS.DCMIType	Text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2010-05
DC.identifier	https://doi.org/10.1089/ten.tea.2009.0532
DC.description	Miho Watanabe, Jun-ichiro Jo, Antonetta Radu, Michio Kaneko, Yasuhiko Tabata, and Alan W. Flake. Tissue Engineering Part A.May 2010.1645-1655. http://doi.org/10.1089/ten.tea.2009.0532.
DCTERMS.abstract	Myelomeningocele (MMC) is a common and devastating malformation. Although fetal surgical closure may improve outcome, a less invasive approach that can be applied earlier in gestation is desirable. The objective of this study was to evaluate the therapeutic feasibility of a tissue engineering approach for prenatal coverage of MMC. A gelatin hydrogel composite combining a gelatin sheet and gelatin sponge was prepared with or without basic fibroblast growth factor incorporation, and applied prenatally to retinoic-acid-induced fetal MMC in the rat model. Most of the composites were adherent to the MMC within the amniotic fluid environment with the help of cyanoacrylate adhesive. Histological examination revealed cells layered over the composites with associated extracellular matrix as well as cellular ingrowth into the sponges. The layer over the composite was composed of mixed nonepithelial and epithelial cells with the extracellular matrix consisting of collagen type I and hyaluronic acid. The tissue inside the sponge consisted of nonepithelial cells and hyaluronic acid. Epidermal ingrowth underneath the sponges and neovascularization into the sponges occurred and were significantly increased by the incorporation of basic fibroblast growth factor. Although further development is needed, this study supports the therapeutic potential of a tissue engineering approach for prenatal coverage of MMC. © 2010 Mary Ann Liebert, Inc.
DC.format	78333
citation_title	A tissue engineering approach for prenatal closure of myelomeningocele with gelatin sponges incorporating basic fibroblast growth factor
citation_author	Watanabe, Miho
	Jo, Jun-ichiro
	Radu, Antonetta
	Kaneko, Michio
	Tabata, Yasuhiko
	Flake, Alan W.
citation_publisher	Mary Ann Liebert Inc.
citation_language	English
citation_date	2010-05
citation_journal_title	Tissue Engineering - Part A
citation_volume	16
citation_issue	5
citation_firstpage	1645
citation_lastpage	1655
citation_doi	https://doi.org/10.1089/ten.tea.2009.0532
citation_pmid	19954327
citation_public_url	https://hdl.handle.net/11094/78333

A tissue engineering approach for prenatal closure of myelomeningocele with gelatin sponges incorporating basic fibroblast growth factor

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