A tissue engineering approach for prenatal closure of myelomeningocele: Comparison of gelatin sponge and microsphere scaffolds and bioactive protein coatings

Watanabe, Miho; Li, Hiaying; Roybal, Jessica; Santore, Matthew; Radu, Antonetta; Jo, Jun-ichiro; Kaneko, Michio; Tabata, Yasuhiko; Flake, Alan

doi:https://doi.org/10.1089/ten.tea.2010.0390

A tissue engineering approach for prenatal closure of myelomeningocele: Comparison of gelatin sponge and microsphere scaffolds and bioactive protein coatings

Watanabe, Miho; Li, Hiaying; Roybal, Jessica et al.

Tissue Engineering - Part A, 2011, 17(7-8), 1099-1110

Number of Access:565（2025-08-16 04:13 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/78334

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Title	A tissue engineering approach for prenatal closure of myelomeningocele: Comparison of gelatin sponge and microsphere scaffolds and bioactive protein coatings
Creator	NRID 1000040791728 1000040791728 researchmap permalink miho miho Scopus Author ID 56282966800 56282966800 ORCID 0000-0001-6691-0611 0000-0001-6691-0611 CreatorName Watanabe, Miho
	CreatorName Li, Hiaying
	CreatorName Roybal, Jessica
	CreatorName Santore, Matthew
	CreatorName Radu, Antonetta
	NRID 1000060511243 1000060511243 CreatorName Jo, Jun-ichiro
	NRID 1000060152807 1000060152807 CreatorName Kaneko, Michio
	NRID 1000050211371 1000050211371 CreatorName Tabata, Yasuhiko
	CreatorName Flake, Alan
Description	Miho Watanabe, Hiaying Li, Jessica Roybal, Matthew Santore, Antonetta Radu, Jun-Ichiro Jo, Michio Kaneko, Yasuhiko Tabata, and Alan Flake. Tissue Engineering Part A.Apr 2011.1099-1110. http://doi.org/10.1089/ten.tea.2010.0390
Abstract	Myelomeningocele (MMC) is a common and devastating malformation. As an alternative to fetal surgical repair, tissue engineering has the potential to provide a less invasive approach for tissue coverage applicable at an earlier stage of gestation. We have previously evaluated the use of gelatin hydrogel composites composed of gelatin sponges and sheets as a platform for tissue coverage of the MMC defect in the retinoic acid induced fetal rat model of MMC. In the current study, we compare our previous composite with gelatin microspheres as a scaffold for tissue ingrowth and cellular adhesion within the amniotic fluid environment. We also examine the relative efficacy of various bioactive protein coatings on the adhesion of amniotic fluid cells to the construct within the amniotic cavity. We conclude from this study that gelatin microspheres are as effective as gelatin sponges as a scaffold for cellular ingrowth and amniotic fluid cell adhesion and that collagen type I and fibronectin coatings enhance amniotic fluid cell adhesion to the gelatin-based scaffolds. These findings support the potential for the development of a tissue-engineered injectable scaffold that could be applied by ultrasound-guided injection, much earlier and less invasively than sponge or sheet-based composites. © 2011 Mary Ann Liebert, Inc.
Publisher	Mary Ann Liebert Inc.
Source Title	Tissue Engineering - Part A
Volume (Issue)	17(7-8)
Page	1099-1110
Date of Issued	2011-04
Language	English
Handle URL	https://hdl.handle.net/11094/78334
PISSN	19373341
eISSN	1937335X
NCID	AA12306003
Relation.isVersionOf	DOI (Publisher Version) https://doi.org/10.1089/ten.tea.2010.0390
Relation.isIdenticalTo	PMID 21128864
Access Rights	open access
oaire:version	Accepted Manuscript
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCMI Type	Text
DCTERMS.bibliographicCitation	Tissue Engineering - Part A. 2011, 17(7-8), p. 1099-1110
DC.title	A tissue engineering approach for prenatal closure of myelomeningocele: Comparison of gelatin sponge and microsphere scaffolds and bioactive protein coatings
DC.creator	Watanabe, Miho
	Li, Hiaying
	Roybal, Jessica
	Santore, Matthew
	Radu, Antonetta
	Jo, Jun-ichiro
	Kaneko, Michio
	Tabata, Yasuhiko
	Flake, Alan
DC.publisher	Mary Ann Liebert Inc.
DC.language' scheme='DCTERMS.RFC1766	English
DC.type' scheme='DCTERMS.DCMIType	Text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2011-04
DC.identifier	https://doi.org/10.1089/ten.tea.2010.0390
DC.description	Miho Watanabe, Hiaying Li, Jessica Roybal, Matthew Santore, Antonetta Radu, Jun-Ichiro Jo, Michio Kaneko, Yasuhiko Tabata, and Alan Flake. Tissue Engineering Part A.Apr 2011.1099-1110. http://doi.org/10.1089/ten.tea.2010.0390
DCTERMS.abstract	Myelomeningocele (MMC) is a common and devastating malformation. As an alternative to fetal surgical repair, tissue engineering has the potential to provide a less invasive approach for tissue coverage applicable at an earlier stage of gestation. We have previously evaluated the use of gelatin hydrogel composites composed of gelatin sponges and sheets as a platform for tissue coverage of the MMC defect in the retinoic acid induced fetal rat model of MMC. In the current study, we compare our previous composite with gelatin microspheres as a scaffold for tissue ingrowth and cellular adhesion within the amniotic fluid environment. We also examine the relative efficacy of various bioactive protein coatings on the adhesion of amniotic fluid cells to the construct within the amniotic cavity. We conclude from this study that gelatin microspheres are as effective as gelatin sponges as a scaffold for cellular ingrowth and amniotic fluid cell adhesion and that collagen type I and fibronectin coatings enhance amniotic fluid cell adhesion to the gelatin-based scaffolds. These findings support the potential for the development of a tissue-engineered injectable scaffold that could be applied by ultrasound-guided injection, much earlier and less invasively than sponge or sheet-based composites. © 2011 Mary Ann Liebert, Inc.
DC.format	78334
citation_title	A tissue engineering approach for prenatal closure of myelomeningocele: Comparison of gelatin sponge and microsphere scaffolds and bioactive protein coatings
citation_author	Watanabe, Miho
	Li, Hiaying
	Roybal, Jessica
	Santore, Matthew
	Radu, Antonetta
	Jo, Jun-ichiro
	Kaneko, Michio
	Tabata, Yasuhiko
	Flake, Alan
citation_publisher	Mary Ann Liebert Inc.
citation_language	English
citation_date	2011-04
citation_journal_title	Tissue Engineering - Part A
citation_volume	17
citation_issue	7-8
citation_firstpage	1099
citation_lastpage	1110
citation_doi	https://doi.org/10.1089/ten.tea.2010.0390
citation_pmid	21128864
citation_public_url	https://hdl.handle.net/11094/78334

A tissue engineering approach for prenatal closure of myelomeningocele: Comparison of gelatin sponge and microsphere scaffolds and bioactive protein coatings

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