Protocadherin-7 contributes to maintenance of bone homeostasis through regulation of osteoclast multinucleation

Kim, Hyunsoo; Takegahara, Noriko; Walsh, Matthew C. et al.

BMB Reports, 2020, 53(9), 472-477

Number of Access:801（2025-08-22 01:03 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/78541

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Title
Protocadherin-7 contributes to maintenance of bone homeostasis through regulation of osteoclast multinucleation
Creator
CreatorName
Kim, Hyunsoo
CreatorName
Takegahara, Noriko
CreatorName
Walsh, Matthew C.
NRID
1000080450394 1000080450394
CreatorName
Ueda, Jun
NRID
1000070578848 1000070578848
CreatorName
Fujihara, Yoshitaka
NRID
1000020304066 1000020304066
ORCID
0000-0001-9859-6217 0000-0001-9859-6217
researchmap permalink
read0054910 read0054910
Scopus Author ID
16689054500 16689054500
CreatorName
Ikawa, Masahito
CreatorName
Choi, Yongwon
Subject
Bone homeostasis
Maturation
Multinucleation
Osteoclast
Pcdh7
Description
Kim, H., Takegahara, N., Walsh, M. C., Ueda, J., Fujihara, Y., Ikawa, M., & Choi, Y. (2020). Protocadherin-7 contributes to maintenance of bone homeostasis through regulation of osteoclast multinucleation. BMB Reports, 53(9), 472-477. doi:10.5483/BMBRep.2020.53.9.050
Abstract
Osteoclasts are hematopoietic-derived cells that resorb bone. They are required to maintain proper bone homeostasis and skeletal strength. Although osteoclast differentiation depends on receptor activator of NF-κB ligand (RANKL) stimulation, additional molecules further contribute to osteoclast maturation. Here, we demonstrate that protocadherin-7 (Pcdh7) regulates formation of multinucleated osteoclasts and contributes to maintenance of bone homeostasis. We found that Pcdh7 expression is induced by RANKL stimulation, and that RNAi-mediated knockdown of Pcdh7 resulted in impaired formation of osteoclasts. We generated Pcdh7-deficient mice and found increased bone mass due to decreased bone resorption but without any defect in bone formation. Using an in vitro culture system, it was revealed that formation of multinucleated osteoclasts is impaired in Pcdh7-deficient cultures, while no apparent defects were observed in differentiation and function of Pcdh7-deficient osteoblasts. Taken together, these results reveal an osteoclast cell-intrinsic role for Pcdh7 in maintaining bone homeostasis.
Publisher
The Biochemical Society of the Republic of Korea
Source Title
BMB Reports
Volume (Issue)
53(9)
Page
472-477
Date of Issued
2020-09-30
Language
English
Handle URL
https://hdl.handle.net/11094/78541
PISSN
19766696
eISSN
1976670X
NCID
AA1231107X
Relation.isIdenticalTo
DOI (Publisher Version)
https://doi.org/10.5483/BMBRep.2020.53.9.050
PMID
32635982
Access Rights
open access
CopyRight
© 2020 Korean Society for Biochemistry and Molecular Biology. This article is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
oaire:version
Version of Record
Category
学術雑誌論文 Journal Article