Testis-enriched kinesin KIF9 is important for progressive motility in mouse spermatozoa

Miyata, Haruhiko; Shimada, Keisuke; Morohoshi, Akane; Oura, Seiya; Matsumura, Takafumi; Xu, Zoulan; Oyama, Yuki; Ikawa, Masahito

doi:https://doi.org/10.1096/fj.201902755R

Testis-enriched kinesin KIF9 is important for progressive motility in mouse spermatozoa

Miyata, Haruhiko; Shimada, Keisuke; Morohoshi, Akane et al.

FASEB Journal, 2020, 34(4), 5389-5400

Number of Access:762（2025-08-22 01:04 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/78548

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Title	Testis-enriched kinesin KIF9 is important for progressive motility in mouse spermatozoa
Creator	NRID 1000050604732 1000050604732 researchmap permalink hmiyata hmiyata Scopus Author ID 37161837000 37161837000 ORCID 0000-0003-4758-5803 0000-0003-4758-5803 CreatorName Miyata, Haruhiko
	NRID 1000060779601 1000060779601 ORCID 0000-0003-3739-7163 0000-0003-3739-7163 researchmap permalink nununununu nununununu Scopus Author ID 56479808000 56479808000 CreatorName Shimada, Keisuke
	CreatorName Morohoshi, Akane
	CreatorName Oura, Seiya
	CreatorName Matsumura, Takafumi
	CreatorName Xu, Zoulan
	CreatorName Oyama, Yuki
	NRID 1000020304066 1000020304066 ORCID 0000-0001-9859-6217 0000-0001-9859-6217 researchmap permalink read0054910 read0054910 Scopus Author ID 16689054500 16689054500 CreatorName Ikawa, Masahito
Subject	fertilization
	male fertility
	sperm motility
Description	Miyata, H., Shimada, K., Morohoshi, A., Oura, S., Matsumura, T., Xu, Z., . . . Ikawa, M. (2020). Testis-enriched kinesin KIF9 is important for progressive motility in mouse spermatozoa. FASEB Journal, 34(4), 5389-5400. doi:10.1096/fj.201902755R
Abstract	Kinesin is a molecular motor that moves along microtubules. Kinesin family member 9 (KIF9) is evolutionarily conserved and expressed strongly in mouse testis. In the unicellular flagellate Chlamydomonas, KLP1 (ortholog of KIF9) is localized to the central pair microtubules of the axoneme and regulates flagellar motility. In contrast, the function of KIF9 remains unclear in mammals. Here, we mutated KIF9 in mice using the CRISPR/Cas9 system. Kif9 mutated mice exhibit impaired sperm motility and subfertility. Further analysis reveals that the flagella lacking KIF9 showed an asymmetric waveform pattern, which leads to a circular motion of spermatozoa. In spermatozoa that lack the central pair protein HYDIN, KIF9 was not detected by immunofluorescence and immunoblot analysis. These results suggest that KIF9 is associated with the central pair microtubules and regulates flagellar motility in mice.
Publisher	John Wiley and Sons Inc.
Source Title	FASEB Journal
Volume (Issue)	34(4)
Page	5389-5400
Date of Issued	2020-04-05
Language	English
Handle URL	https://hdl.handle.net/11094/78548
PISSN	08926638
eISSN	15306860
NCID	AA1066874X
Relation.isIdenticalTo	DOI (Publisher Version) https://doi.org/10.1096/fj.201902755R
Relation.isIdenticalTo	PMID 32072696
Access Rights	open access
CopyRight	© 2020 The Authors. The FASEB Journal published by Wiley Periodicals, Inc. on behalf of Federation of American Societies for Experimental Biology. This article is licensed under a Creative Commons Attribution 4.0 International License.
oaire:version	Version of Record
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCTERMS.bibliographicCitation	FASEB Journal. 2020, 34(4), p. 5389-5400
DC.title	Testis-enriched kinesin KIF9 is important for progressive motility in mouse spermatozoa
DC.creator	Miyata, Haruhiko
	Shimada, Keisuke
	Morohoshi, Akane
	Oura, Seiya
	Matsumura, Takafumi
	Xu, Zoulan
	Oyama, Yuki
	Ikawa, Masahito
DC.publisher	John Wiley and Sons Inc.
DC.language' scheme='DCTERMS.RFC1766	English
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2020-04-05
DC.identifier	https://doi.org/10.1096/fj.201902755R
DC.subject	fertilization
	male fertility
	sperm motility
DC.description	Miyata, H., Shimada, K., Morohoshi, A., Oura, S., Matsumura, T., Xu, Z., . . . Ikawa, M. (2020). Testis-enriched kinesin KIF9 is important for progressive motility in mouse spermatozoa. FASEB Journal, 34(4), 5389-5400. doi:10.1096/fj.201902755R
DCTERMS.abstract	Kinesin is a molecular motor that moves along microtubules. Kinesin family member 9 (KIF9) is evolutionarily conserved and expressed strongly in mouse testis. In the unicellular flagellate Chlamydomonas, KLP1 (ortholog of KIF9) is localized to the central pair microtubules of the axoneme and regulates flagellar motility. In contrast, the function of KIF9 remains unclear in mammals. Here, we mutated KIF9 in mice using the CRISPR/Cas9 system. Kif9 mutated mice exhibit impaired sperm motility and subfertility. Further analysis reveals that the flagella lacking KIF9 showed an asymmetric waveform pattern, which leads to a circular motion of spermatozoa. In spermatozoa that lack the central pair protein HYDIN, KIF9 was not detected by immunofluorescence and immunoblot analysis. These results suggest that KIF9 is associated with the central pair microtubules and regulates flagellar motility in mice.
DC.rights	© 2020 The Authors. The FASEB Journal published by Wiley Periodicals, Inc. on behalf of Federation of American Societies for Experimental Biology. This article is licensed under a Creative Commons Attribution 4.0 International License.
DC.format	78548
citation_title	Testis-enriched kinesin KIF9 is important for progressive motility in mouse spermatozoa
citation_author	Miyata, Haruhiko
	Shimada, Keisuke
	Morohoshi, Akane
	Oura, Seiya
	Matsumura, Takafumi
	Xu, Zoulan
	Oyama, Yuki
	Ikawa, Masahito
citation_publisher	John Wiley and Sons Inc.
citation_language	English
citation_date	2020-04-05
citation_journal_title	FASEB Journal
citation_volume	34
citation_issue	4
citation_firstpage	5389
citation_lastpage	5400
citation_doi	https://doi.org/10.1096/fj.201902755R
citation_pmid	32072696
citation_public_url	https://hdl.handle.net/11094/78548

Testis-enriched kinesin KIF9 is important for progressive motility in mouse spermatozoa

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