Lvrn expression is not critical for mouse placentation

Tobita, Tomohiro; Kiyozumi, Daiji; Muto, Masanaga et al.

Journal of Reproduction and Development, 2019, 65(3), 239-244

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Identifier to cite or link to this item: https://hdl.handle.net/11094/78561

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Title
Lvrn expression is not critical for mouse placentation
Creator
CreatorName
Tobita, Tomohiro
NRID
1000070452430 1000070452430
ORCID
0000-0003-3289-227X 0000-0003-3289-227X
researchmap permalink
dkiyozumi dkiyozumi
Scopus Author ID
6506341053 6506341053
CreatorName
Kiyozumi, Daiji
CreatorName
Muto, Masanaga
NRID
1000050712551 1000050712551
CreatorName
Noda, Taichi
NRID
1000020304066 1000020304066
ORCID
0000-0001-9859-6217 0000-0001-9859-6217
researchmap permalink
read0054910 read0054910
Scopus Author ID
16689054500 16689054500
CreatorName
Ikawa, Masahito
Subject
Knockout
Lentivirus
Trophoblast
Description
Tomohiro TOBITA, Daiji KIYOZUMI, Masanaga MUTO, Taichi NODA, Masahito IKAWA, Lvrn expression is not critical for mouse placentation, Journal of Reproduction and Development, 2019, Volume 65, Issue 3, Pages 239-244, Released June 14, 2019, [Advance publication] Released February 10, 2019, Online ISSN 1348-4400, Print ISSN 0916-8818, https://doi.org/10.1262/jrd.2018-157, https://www.jstage.jst.go.jp/article/jrd/65/3/65_2018-157/_article/-char/en
Abstract
Preeclampsia is a systemic disease caused by abnormal placentation that affects both mother and fetus. It was reported that Laeverin (LVRN, also known as Aminopeptidase Q) was up-regulated in the placenta of preeclamptic patients. However, physiological and pathological functions of LVRN remained to be unknown. Here we characterized Lvrn function during placentation in mice. RT-PCR showed that Lvrn is expressed in both fetus and placenta during embryogenesis, and several adult tissues. When we overexpressed Lvrn in a placenta-specific manner using lentiviral vectors, we did not see any defects in both placentae and fetuses. The mice carrying Lvrn overexpressing placentas did not show any preeclampsialike symptoms such as maternal high blood pressure and fetal growth restriction. We next ablated Lvrn by CRISPR/Cas9- mediated genome editing to see physiological function. In Lvrn ablated mice, maternal blood pressure during pregnancy was not affected, and both placentas and fetuses grew normally. Collectively, these results suggest that, LVRN is irrelevant to preeclampsia and dispensable for normal placentation and embryonic development in mice.
Publisher
The Japanese Society of Animal Reproduction (JSAR)
Source Title
Journal of Reproduction and Development
Volume (Issue)
65(3)
Page
239-244
Date of Issued
2019
Language
English
Handle URL
https://hdl.handle.net/11094/78561
PISSN
09168818
eISSN
13484400
NCID
AA12018734
Relation.isIdenticalTo
DOI (Publisher Version)
https://doi.org/10.1262/jrd.2018-157
PMID
30745494
Access Rights
open access
CopyRight
© 2019 the Society for Reproduction and Development. This article is licensed under a Creative Commons Attribution 4.0 International License.
oaire:version
Version of Record
Category
学術雑誌論文 Journal Article