PTBP1 contributes to spermatogenesis through regulation of proliferation in spermatogonia

Senoo, Manami; Takijiri, Takashi; Yoshida, Nobuaki et al.

Journal of Reproduction and Development, 2019, 65(1), 37-46

Number of Access:238（2025-05-16 14:45 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/78563

Link to primary information 

File Format Terms of use Size Views Date.Available Description information
JReprodDev_65_01_37 pdf None 6.48 MB 147 2021.02.08  
Creative Commons : Attribution

Item Information

Output File Export EndNote Basic Export Mendeley

Title
PTBP1 contributes to spermatogenesis through regulation of proliferation in spermatogonia
Creator
CreatorName
Senoo, Manami
CreatorName
Takijiri, Takashi
NRID
1000010250341 1000010250341
CreatorName
Yoshida, Nobuaki
NRID
1000080608787 1000080608787
CreatorName
Ozawa, Manabu
NRID
1000020304066 1000020304066
ORCID
0000-0001-9859-6217 0000-0001-9859-6217
researchmap permalink
read0054910 read0054910
Scopus Author ID
16689054500 16689054500
CreatorName
Ikawa, Masahito
Subject
Alternative splicing
Polypyrimidine track-binding protein
RNA-binding protein
Spermatogenesis
Spermatogonia proliferation
Description
Manami SENOO, Takashi TAKIJIRI, Nobuaki YOSHIDA, Manabu OZAWA, Masahito IKAWA, PTBP1 contributes to spermatogenesis through regulation of proliferation in spermatogonia, Journal of Reproduction and Development, 2019, Volume 65, Issue 1, Pages 37-46, Released February 08, 2019, [Advance publication] Released November 12, 2018, Online ISSN 1348-4400, Print ISSN 0916-8818, https://doi.org/10.1262/jrd.2018-109, https://www.jstage.jst.go.jp/article/jrd/65/1/65_2018-109/_article/-char/en
Abstract
Polypyrimidine tract-binding protein 1 (PTBP1) is a highly conserved RNA-binding protein that is a well-known regulator of alternative splicing. Testicular tissue is one of the richest tissues with respect to the number of alternative splicing mRNA isoforms, but the molecular role(s) of PTBP1 in the regulation of these isoforms during spermatogenesis is still unclear. Here, we developed a germ cell-specific Ptbp1 conditional knockout (cKO) mouse model by using the Cre-loxP system to investigate the role of PTBP1 in spermatogenesis. Testis weight in Ptbp1 cKO mice was comparable to that in age-matched controls until 3 weeks of age; at ≥ 2 months old, testis weight was significantly lighter in cKO mice than in age-matched controls. Sperm count in Ptbp1 cKO mice at 2 months old was comparable to that in controls, whereas sperm count significantly decreased at 6 months old. Seminiferous tubules that exhibited degeneration in spermatogenic function were more evident in the 2-month-old Ptbp1 cKO mice than in controls. In addition, the early neonatal proliferation of spermatogonia, during postnatal days 1-5, was significantly retarded in Ptbp1 cKO mice compared with that in controls. An in vitro spermatogonia culture model (germline stem cells) revealed that hydroxytamoxifen-induced deletion of PTBP1 from germline stem cells caused severe proliferation arrest accompanied by an increase of apoptotic cell death. These data suggest that PTBP1 contributes to spermatogenesis through regulation of spermatogonia proliferation.
Publisher
The Japanese Society of Animal Reproduction (JSAR)
Source Title
Journal of Reproduction and Development
Volume (Issue)
65(1)
Page
37-46
Date of Issued
2019
Language
English
Handle URL
https://hdl.handle.net/11094/78563
PISSN
09168818
eISSN
13484400
NCID
AA12018734
Relation.isIdenticalTo
DOI (Publisher Version)
https://doi.org/10.1262/jrd.2018-109
PMID
30416150
Access Rights
open access
CopyRight
© 2019 the Society for Reproduction and Development. This article is licensed under a Creative Commons Attribution 4.0 International License.
oaire:version
Version of Record
Category
学術雑誌論文 Journal Article