Nine genes abundantly expressed in the epididymis are not essential for male fecundity in mice

Noda, T.; Sakurai, N.; Nozawa, K. et al.

Andrology, 2019, 7(5), 644-653

Number of Access:894（2025-08-22 01:04 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/78564

Link to primary information 

File Format Terms of use Size Views Date.Available Description information
Andrology_07_05_644 pdf None 4.36 MB 172 2021.02.08  
Creative Commons : Attribution

Item Information

Output File Export EndNote Basic Export Mendeley

Title
Nine genes abundantly expressed in the epididymis are not essential for male fecundity in mice
Creator
NRID
1000050712551 1000050712551
CreatorName
Noda, T.
CreatorName
Sakurai, N.
CreatorName
Nozawa, K.
CreatorName
Kobayashi, S.
CreatorName
Devlin, D. J.
CreatorName
Matzuk, M. M.
NRID
1000020304066 1000020304066
ORCID
0000-0001-9859-6217 0000-0001-9859-6217
researchmap permalink
read0054910 read0054910
Scopus Author ID
16689054500 16689054500
CreatorName
Ikawa, M.
Subject
genetically modified mice
genome editing
sperm maturation
Description
Noda, T., Sakurai, N., Nozawa, K., Kobayashi, S., Devlin, D. J., Matzuk, M. M., & Ikawa, M. (2019). Nine genes abundantly expressed in the epididymis are not essential for male fecundity in mice. Andrology, 7(5), 644-653. doi:10.1111/andr.12621
Abstract
Background: Spermatozoa become competent for fertilization during transit through the epididymis. As spermatozoa from the proximal caudal epididymis can fertilize eggs, proteins from the caput and corpus epididymis are required for sperm maturation. Objectives: Microarray analysis identified that more than 17,000 genes are expressed in the epididymis; however, few of these genes demonstrate expression restricted to the epididymis. To analyze epididymis-enriched gene function in vivo, we generated knockout (KO) mutations in nine genes that are abundantly expressed in the caput and corpus region of the epididymis. Materials and methods: KO mice were generated using the CRISPR/Cas9 system. The histology of the epididymis was observed with hematoxylin and eosin staining. KO males were caged with wild-type females for 3–6 months to check fertility. Results: We generated individual mutant mouse lines having indel mutations in Pate1, Pate2, or Pate3. We also deleted the coding regions of Clpsl2, Epp13, and Rnase13, independently. Finally, the 150 kb region encoding Gm1110, Glb1l2, and Glb1l3 was deleted to generate a triple KO mouse line. Histology of the epididymis and sperm morphology of all KO lines were comparable to control males. The females mated with these KO males delivered pups at comparable numbers as control males. Discussion and conclusion: We revealed that nine genes abundantly expressed in the caput and corpus epididymis are dispensable for sperm function and male fecundity. CRISPR/Cas9-mediated KO mice generation accelerates the screening of epididymis-enriched genes for potential functions in reproduction.
Publisher
Blackwell Publishing Ltd
Source Title
Andrology
Volume (Issue)
7(5)
Page
644-653
Date of Issued
2019-08-09
Language
English
Handle URL
https://hdl.handle.net/11094/78564
PISSN
20472919
eISSN
20472927
NCID
AA12787207
Relation.isIdenticalTo
DOI (Publisher Version)
https://doi.org/10.1111/andr.12621
PMID
30927342
Access Rights
open access
CopyRight
© 2019 The Authors. Andrology published by John Wiley & Sons Ltd on behalf of American Society of Andrology and European Academy of Andrology. This article is licensed under a Creative Commons Attribution 4.0 International License.
oaire:version
Version of Record
Category
学術雑誌論文 Journal Article