Co-expression of sperm membrane proteins CMTM2A and CMTM2B is essential for ADAM3 localization and male fertility in mice

Fujihara, Yoshitaka; Oji, Asami; Kojima, Kanako et al.

Journal of Cell Science, 2018, 131(19), jcs.221481

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Identifier to cite or link to this item: https://hdl.handle.net/11094/78568

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Title
Co-expression of sperm membrane proteins CMTM2A and CMTM2B is essential for ADAM3 localization and male fertility in mice
Creator
NRID
1000070578848 1000070578848
CreatorName
Fujihara, Yoshitaka
NRID
1000050803417 1000050803417
CreatorName
Oji, Asami
NRID
1000060813631 1000060813631
researchmap permalink
kojima-kita-kanako kojima-kita-kanako
Scopus Author ID
36008685400 36008685400
CreatorName
Kojima, Kanako
Creator.Alternative
Kita, Kanako
CreatorName
Larasati, Tamara
NRID
1000020304066 1000020304066
ORCID
0000-0001-9859-6217 0000-0001-9859-6217
researchmap permalink
read0054910 read0054910
Scopus Author ID
16689054500 16689054500
CreatorName
Ikawa, Masahito
Subject
CKLF
CMTM gene cluster
CRISPR/Cas9
Fertilization
Testis
Zona pellucida
Abstract
Chemokines are signaling proteins that are secreted to induce chemotaxis during an immunological response. However, the functions of transmembrane-type chemokine-like factor (CKLF) and the CMTM (CKLF-like MARVEL transmembrane domain containing) protein family remain to be determined. In this study, we focused on the testis-specific mouse CMTM gene cluster (Cmtm1, Cmtm2a and Cmtm2b) and generated CRISPR/Cas9-mediated mutant mice to examine their physiological functions. Although Cmtm1 mutant mice were fertile, Cmtm2a and Cmtm2b double mutant mice had defects in male fertility due to impaired sperm function. We found that co-expression of sperm membrane proteins CMTM2A and CMTM2B is required for male fertility and affects the localization of the sperm membrane protein ADAM3 in regulating sperm fertilizing ability.
Publisher
Company of Biologists Ltd
Source Title
Journal of Cell Science
Volume (Issue)
131(19)
Page
jcs.221481
Date of Issued
2018-10-08
Language
English
Handle URL
https://hdl.handle.net/11094/78568
PISSN
00219533
eISSN
14779137
NCID
AA11720335
Relation.isIdenticalTo
DOI (Publisher Version)
https://doi.org/10.1242/jcs.221481
PMID
30209135
Access Rights
open access
oaire:version
Version of Record
Category
学術雑誌論文 Journal Article