Revolutionizing male fertility factor research in mice by using the genome editing tool CRISPR/Cas9

Abbasi, Ferheen; Miyata, Haruhiko; Ikawa, Masahito

Reproductive Medicine and Biology, 2018, 17(1), 3-10

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Identifier to cite or link to this item: https://hdl.handle.net/11094/78575

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Title
Revolutionizing male fertility factor research in mice by using the genome editing tool CRISPR/Cas9
Creator
CreatorName
Abbasi, Ferheen
NRID
1000050604732 1000050604732
researchmap permalink
hmiyata hmiyata
Scopus Author ID
37161837000 37161837000
ORCID
0000-0003-4758-5803 0000-0003-4758-5803
CreatorName
Miyata, Haruhiko
NRID
1000020304066 1000020304066
ORCID
0000-0001-9859-6217 0000-0001-9859-6217
researchmap permalink
read0054910 read0054910
Scopus Author ID
16689054500 16689054500
CreatorName
Ikawa, Masahito
Subject
CRISPR/Cas9
fertility
gene editing
reproduction
spermatozoa
Description
Abbasi, F, Miyata, H, Ikawa, M. Revolutionizing male fertility factor research in mice by using the genome editing tool, CRISPR/Cas9. Reprod Med Biol. 2018; 17: 3‐ 10. https://doi.org/10.1002/rmb2.12067
Abstract
Background: Reproductive research is quintessential in understanding not only the cause of infertility, but also for creating family planning tools. The knockout (KO) system approach is conducive to discovering genes that are essential for fertility in mice. However, in vivo research has been limited due to its high cost and length of time needed to establish KO mice. Methods: The mechanisms behind the CRISPR/Cas9 system and its application in investigating male fertility in mice are described by using original and review articles. Results: The CRISPR/CAS9 SYSTEM has enabled researchers to rapidly, efficiently, and inexpensively produce genetically modified mice to study male fertility. Several genes have been highlighted that were found to be indispensable for male fertility by using the CRISPR/Cas9 system, as well as more complicated gene manipulation techniques, such as point mutations, tag insertions, and double knockouts, which have become easier with this new technology. Conclusion: In order to increase efficiency and usage, new methods of CRISPR/Cas9 integration are being developed, such as electroporation and applying the system to embryonic stem cells. The hidden mysteries of male fertility will be unraveled with the help of this new technology.
Publisher
John Wiley and Sons Ltd
Source Title
Reproductive Medicine and Biology
Volume (Issue)
17(1)
Page
3-10
Date of Issued
2018-01-16
Language
English
Handle URL
https://hdl.handle.net/11094/78575
PISSN
14455781
eISSN
14470578
NCID
AA11706516
Relation.isIdenticalTo
DOI (Publisher Version)
https://doi.org/10.1002/rmb2.12067
Access Rights
open access
CopyRight
© 2017 The Authors. Reproductive Medicine and Biology published by John Wiley & Sons Australia, Ltd on behalf of Japan Society for Reproductive Medicine. This article is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
oaire:version
Version of Record
Category
学術雑誌論文 Journal Article