Testis-Specific Histone Variant H3t Gene Is Essential for Entry into Spermatogenesis

Ueda, Jun; Harada, Akihito; Urahama, Takashi; Machida, Shinichi; Maehara, Kazumitsu; Hada, Masashi; Makino, Yoshinori; Nogami, Jumpei; Horikoshi, Naoki; Osakabe, Akihisa; Taguchi, Hiroyuki; Tanaka, Hiroki; Tachiwana, Hiroaki; Yao, Tatsuma; Yamada, Minami; Iwamoto, Takashi; Isotani, Ayako; Ikawa, Masahito; Tachibana, Taro; Okada, Yuki; Kimura, Hiroshi; Ohkawa, Yasuyuki; Kurumizaka, Hitoshi; Yamagata, Kazuo

doi:https://doi.org/10.1016/j.celrep.2016.12.065

Testis-Specific Histone Variant H3t Gene Is Essential for Entry into Spermatogenesis

Ueda, Jun; Harada, Akihito; Urahama, Takashi et al.

Cell Reports, 2017, 18(3), 593-600

Number of Access:3,685（2025-08-22 02:12 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/78582

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Title	Testis-Specific Histone Variant H3t Gene Is Essential for Entry into Spermatogenesis
Creator	NRID 1000080450394 1000080450394 CreatorName Ueda, Jun
	NRID 1000060596823 1000060596823 CreatorName Harada, Akihito
	CreatorName Urahama, Takashi
	NRID 1000050732195 1000050732195 CreatorName Machida, Shinichi
	NRID 1000090726431 1000090726431 CreatorName Maehara, Kazumitsu
	NRID 1000010802746 1000010802746 CreatorName Hada, Masashi
	CreatorName Makino, Yoshinori
	NRID 1000060801751 1000060801751 CreatorName Nogami, Jumpei
	CreatorName Horikoshi, Naoki
	NRID 1000070632107 1000070632107 CreatorName Osakabe, Akihisa
	CreatorName Taguchi, Hiroyuki
	NRID 1000090880104 1000090880104 CreatorName Tanaka, Hiroki
	NRID 1000070546382 1000070546382 CreatorName Tachiwana, Hiroaki
	CreatorName Yao, Tatsuma
	CreatorName Yamada, Minami
	NRID 1000060223426 1000060223426 CreatorName Iwamoto, Takashi
	NRID 1000020444523 1000020444523 CreatorName Isotani, Ayako
	NRID 1000020304066 1000020304066 ORCID 0000-0001-9859-6217 0000-0001-9859-6217 researchmap permalink read0054910 read0054910 Scopus Author ID 16689054500 16689054500 CreatorName Ikawa, Masahito
	NRID 1000080311752 1000080311752 CreatorName Tachibana, Taro
	NRID 1000060546430 1000060546430 CreatorName Okada, Yuki
	NRID 1000030241392 1000030241392 CreatorName Kimura, Hiroshi
	NRID 1000080448430 1000080448430 CreatorName Ohkawa, Yasuyuki
	NRID 1000080300870 1000080300870 CreatorName Kurumizaka, Hitoshi
	NRID 1000010361312 1000010361312 CreatorName Yamagata, Kazuo
Subject	chromatin
	crystal structure
	epigenetics
	histone variant
	meiosis
	nucleosome
	spermatogenesis
	spermatogonial differentiation
	spermatozoa
	testes
Description	Jun Ueda, Akihito Harada, Takashi Urahama, Shinichi Machida, Kazumitsu Maehara, Masashi Hada, Yoshinori Makino, Jumpei Nogami, Naoki Horikoshi, Akihisa Osakabe, Hiroyuki Taguchi, Hiroki Tanaka, Hiroaki Tachiwana, Tatsuma Yao, Minami Yamada, Takashi Iwamoto, Ayako Isotani, Masahito Ikawa, Taro Tachibana, Yuki Okada, Hiroshi Kimura, Yasuyuki Ohkawa, Hitoshi Kurumizaka, Kazuo Yamagata, Testis-Specific Histone Variant H3t Gene Is Essential for Entry into Spermatogenesis, Cell Reports, Volume 18, Issue 3, 2017, Pages 593-600, ISSN 2211-1247, https://doi.org/10.1016/j.celrep.2016.12.065.
Abstract	Cellular differentiation is associated with dynamic chromatin remodeling in establishing a cell-type-specific epigenomic landscape. Here, we find that mouse testis-specific and replication-dependent histone H3 variant H3t is essential for very early stages of spermatogenesis. H3t gene deficiency leads to azoospermia because of the loss of haploid germ cells. When differentiating spermatogonia emerge in normal spermatogenesis, H3t appears and replaces the canonical H3 proteins. Structural and biochemical analyses reveal that H3t-containing nucleosomes are more flexible than the canonical nucleosomes. Thus, by incorporating H3t into the genome during spermatogonial differentiation, male germ cells are able to enter meiosis and beyond.
Publisher	Elsevier B.V.
Source Title	Cell Reports
Volume (Issue)	18(3)
Page	593-600
Date of Issued	2017-01-17
Language	English
Handle URL	https://hdl.handle.net/11094/78582
eISSN	22111247
Relation.isIdenticalTo	DOI (Publisher Version) https://doi.org/10.1016/j.celrep.2016.12.065
Relation.isIdenticalTo	PMID 28099840
Access Rights	open access
CopyRight	© 2016 The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License.
oaire:version	Version of Record
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCTERMS.bibliographicCitation	Cell Reports. 2017, 18(3), p. 593-600
DC.title	Testis-Specific Histone Variant H3t Gene Is Essential for Entry into Spermatogenesis
DC.creator	Ueda, Jun
	Harada, Akihito
	Urahama, Takashi
	Machida, Shinichi
	Maehara, Kazumitsu
	Hada, Masashi
	Makino, Yoshinori
	Nogami, Jumpei
	Horikoshi, Naoki
	Osakabe, Akihisa
	Taguchi, Hiroyuki
	Tanaka, Hiroki
	Tachiwana, Hiroaki
	Yao, Tatsuma
	Yamada, Minami
	Iwamoto, Takashi
	Isotani, Ayako
	Ikawa, Masahito
	Tachibana, Taro
	Okada, Yuki
	Kimura, Hiroshi
	Ohkawa, Yasuyuki
	Kurumizaka, Hitoshi
	Yamagata, Kazuo
DC.publisher	Elsevier B.V.
DC.language' scheme='DCTERMS.RFC1766	English
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2017-01-17
DC.identifier	https://doi.org/10.1016/j.celrep.2016.12.065
DC.subject	chromatin
	crystal structure
	epigenetics
	histone variant
	meiosis
	nucleosome
	spermatogenesis
	spermatogonial differentiation
	spermatozoa
	testes
DC.description	Jun Ueda, Akihito Harada, Takashi Urahama, Shinichi Machida, Kazumitsu Maehara, Masashi Hada, Yoshinori Makino, Jumpei Nogami, Naoki Horikoshi, Akihisa Osakabe, Hiroyuki Taguchi, Hiroki Tanaka, Hiroaki Tachiwana, Tatsuma Yao, Minami Yamada, Takashi Iwamoto, Ayako Isotani, Masahito Ikawa, Taro Tachibana, Yuki Okada, Hiroshi Kimura, Yasuyuki Ohkawa, Hitoshi Kurumizaka, Kazuo Yamagata, Testis-Specific Histone Variant H3t Gene Is Essential for Entry into Spermatogenesis, Cell Reports, Volume 18, Issue 3, 2017, Pages 593-600, ISSN 2211-1247, https://doi.org/10.1016/j.celrep.2016.12.065.
DCTERMS.abstract	Cellular differentiation is associated with dynamic chromatin remodeling in establishing a cell-type-specific epigenomic landscape. Here, we find that mouse testis-specific and replication-dependent histone H3 variant H3t is essential for very early stages of spermatogenesis. H3t gene deficiency leads to azoospermia because of the loss of haploid germ cells. When differentiating spermatogonia emerge in normal spermatogenesis, H3t appears and replaces the canonical H3 proteins. Structural and biochemical analyses reveal that H3t-containing nucleosomes are more flexible than the canonical nucleosomes. Thus, by incorporating H3t into the genome during spermatogonial differentiation, male germ cells are able to enter meiosis and beyond.
DC.rights	© 2016 The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License.
DC.format	78582
citation_title	Testis-Specific Histone Variant H3t Gene Is Essential for Entry into Spermatogenesis
citation_author	Ueda, Jun
	Harada, Akihito
	Urahama, Takashi
	Machida, Shinichi
	Maehara, Kazumitsu
	Hada, Masashi
	Makino, Yoshinori
	Nogami, Jumpei
	Horikoshi, Naoki
	Osakabe, Akihisa
	Taguchi, Hiroyuki
	Tanaka, Hiroki
	Tachiwana, Hiroaki
	Yao, Tatsuma
	Yamada, Minami
	Iwamoto, Takashi
	Isotani, Ayako
	Ikawa, Masahito
	Tachibana, Taro
	Okada, Yuki
	Kimura, Hiroshi
	Ohkawa, Yasuyuki
	Kurumizaka, Hitoshi
	Yamagata, Kazuo
citation_publisher	Elsevier B.V.
citation_language	English
citation_date	2017-01-17
citation_journal_title	Cell Reports
citation_volume	18
citation_issue	3
citation_firstpage	593
citation_lastpage	600
citation_doi	https://doi.org/10.1016/j.celrep.2016.12.065
citation_pmid	28099840
citation_public_url	https://hdl.handle.net/11094/78582

Testis-Specific Histone Variant H3t Gene Is Essential for Entry into Spermatogenesis

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