Structural and functional insights into IZUMO1 recognition by JUNO in mammalian fertilization

Kato, Kazuki; Satouh, Yuhkoh; Nishimasu, Hiroshi; Kurabayashi, Arisa; Morita, Junko; Fujihara, Yoshitaka; Oji, Asami; Ishitani, Ryuichiro; Ikawa, Masahito; Nureki, Osamu

doi:https://doi.org/10.1038/ncomms12198

Structural and functional insights into IZUMO1 recognition by JUNO in mammalian fertilization

Kato, Kazuki; Satouh, Yuhkoh; Nishimasu, Hiroshi et al.

Nature Communications, 2016, 7, 12198

Number of Access:986（2025-08-22 01:08 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/78586

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Title	Structural and functional insights into IZUMO1 recognition by JUNO in mammalian fertilization
Creator	NRID 1000010903776 1000010903776 CreatorName Kato, Kazuki
	NRID 1000040545571 1000040545571 CreatorName Satouh, Yuhkoh
	NRID 1000000467044 1000000467044 CreatorName Nishimasu, Hiroshi
	CreatorName Kurabayashi, Arisa
	CreatorName Morita, Junko
	NRID 1000070578848 1000070578848 CreatorName Fujihara, Yoshitaka
	NRID 1000050803417 1000050803417 CreatorName Oji, Asami
	CreatorName Ishitani, Ryuichiro
	NRID 1000020304066 1000020304066 ORCID 0000-0001-9859-6217 0000-0001-9859-6217 researchmap permalink read0054910 read0054910 Scopus Author ID 16689054500 16689054500 CreatorName Ikawa, Masahito
	NRID 1000010272460 1000010272460 CreatorName Nureki, Osamu
Description	Kato, K., Satouh, Y., Nishimasu, H. et al. Structural and functional insights into IZUMO1 recognition by JUNO in mammalian fertilization. Nat Commun 7, 12198 (2016). https://doi.org/10.1038/ncomms12198
Abstract	Sperm-egg fusion is the critical step in mammalian fertilization, and requires the interaction between IZUMO1 on the sperm surface and JUNO (also known as folate receptor (FR) 4 or IZUMO1R) on the egg surface. Whereas other FRs bind and uptake folates, JUNO binds IZUMO1 and establishes the cell-cell adhesion. However, the mechanism of IZUMO1 recognition by JUNO has remained elusive. Here we report the crystal structure of mouse JUNO, at 2.3 Å resolution. A structural comparison of JUNO with the FRs revealed that JUNO and the FRs have similar overall structures, but JUNO lacks the folate-binding pocket, thereby explaining the inability of JUNO to bind folate. Further complementation of Juno knockout eggs with mutant Juno messenger RNAs revealed that the conserved, surface-exposed tryptophan residue of JUNO is required for sperm binding and fertilization. Our structure-based in vivo functional analyses provide a framework towards a mechanistic understanding of mammalian gamete recognition.
Publisher	Nature Research
Source Title	Nature Communications
Volume (Issue)	7
Page	12198
Date of Issued	2016-07-15
Language	English
Handle URL	https://hdl.handle.net/11094/78586
eISSN	20411723
NCID	AA12645905
Relation.isIdenticalTo	DOI (Publisher Version) https://doi.org/10.1038/ncomms12198
Relation.isIdenticalTo	PMID 27416963
Access Rights	open access
CopyRight	© 2016 The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License.
oaire:version	Version of Record
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCTERMS.bibliographicCitation	Nature Communications. 2016, 7, p. 12198
DC.title	Structural and functional insights into IZUMO1 recognition by JUNO in mammalian fertilization
DC.creator	Kato, Kazuki
	Satouh, Yuhkoh
	Nishimasu, Hiroshi
	Kurabayashi, Arisa
	Morita, Junko
	Fujihara, Yoshitaka
	Oji, Asami
	Ishitani, Ryuichiro
	Ikawa, Masahito
	Nureki, Osamu
DC.publisher	Nature Research
DC.language' scheme='DCTERMS.RFC1766	English
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2016-07-15
DC.identifier	https://doi.org/10.1038/ncomms12198
DC.description	Kato, K., Satouh, Y., Nishimasu, H. et al. Structural and functional insights into IZUMO1 recognition by JUNO in mammalian fertilization. Nat Commun 7, 12198 (2016). https://doi.org/10.1038/ncomms12198
DCTERMS.abstract	Sperm-egg fusion is the critical step in mammalian fertilization, and requires the interaction between IZUMO1 on the sperm surface and JUNO (also known as folate receptor (FR) 4 or IZUMO1R) on the egg surface. Whereas other FRs bind and uptake folates, JUNO binds IZUMO1 and establishes the cell-cell adhesion. However, the mechanism of IZUMO1 recognition by JUNO has remained elusive. Here we report the crystal structure of mouse JUNO, at 2.3 Å resolution. A structural comparison of JUNO with the FRs revealed that JUNO and the FRs have similar overall structures, but JUNO lacks the folate-binding pocket, thereby explaining the inability of JUNO to bind folate. Further complementation of Juno knockout eggs with mutant Juno messenger RNAs revealed that the conserved, surface-exposed tryptophan residue of JUNO is required for sperm binding and fertilization. Our structure-based in vivo functional analyses provide a framework towards a mechanistic understanding of mammalian gamete recognition.
DC.rights	© 2016 The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License.
DC.format	78586
citation_title	Structural and functional insights into IZUMO1 recognition by JUNO in mammalian fertilization
citation_author	Kato, Kazuki
	Satouh, Yuhkoh
	Nishimasu, Hiroshi
	Kurabayashi, Arisa
	Morita, Junko
	Fujihara, Yoshitaka
	Oji, Asami
	Ishitani, Ryuichiro
	Ikawa, Masahito
	Nureki, Osamu
citation_publisher	Nature Research
citation_language	English
citation_date	2016-07-15
citation_journal_title	Nature Communications
citation_volume	7
citation_firstpage	12198
citation_doi	https://doi.org/10.1038/ncomms12198
citation_pmid	27416963
citation_public_url	https://hdl.handle.net/11094/78586

Structural and functional insights into IZUMO1 recognition by JUNO in mammalian fertilization

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