Essential role of autoactivation circuitry on Aurora B-mediated H2AX-pS121 in mitosis

Shimada, Midori; Goshima, Takahiro; Matsuo, Hiromi; Johmura, Yoshikazu; Haruta, Mayumi; Murata, Kazuhiro; Tanaka, Hiromitsu; Ikawa, Masahito; Nakanishi, Keiko; Nakanishi, Makoto

doi:https://doi.org/10.1038/ncomms12059

Essential role of autoactivation circuitry on Aurora B-mediated H2AX-pS121 in mitosis

Shimada, Midori; Goshima, Takahiro; Matsuo, Hiromi et al.

Nature Communications, 2016, 7, 12059

Number of Access:961（2025-08-22 01:06 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/78587

Link to primary information

File	Format	Terms of use	Size	Views	Date.Available	Description	information
NatCommun_07_12059	pdf	None	2.08 MB	131	2021.02.08

Creative Commons : Attribution

Item Information

Output File Export EndNote Basic Export Mendeley

Title	Essential role of autoactivation circuitry on Aurora B-mediated H2AX-pS121 in mitosis
Creator	NRID 1000060444981 1000060444981 CreatorName Shimada, Midori
	CreatorName Goshima, Takahiro
	CreatorName Matsuo, Hiromi
	NRID 1000040616322 1000040616322 CreatorName Johmura, Yoshikazu
	CreatorName Haruta, Mayumi
	CreatorName Murata, Kazuhiro
	NRID 1000010263310 1000010263310 CreatorName Tanaka, Hiromitsu
	NRID 1000020304066 1000020304066 ORCID 0000-0001-9859-6217 0000-0001-9859-6217 researchmap permalink read0054910 read0054910 Scopus Author ID 16689054500 16689054500 CreatorName Ikawa, Masahito
	NRID 1000050280813 1000050280813 CreatorName Nakanishi, Keiko
	NRID 1000040217774 1000040217774 CreatorName Nakanishi, Makoto
Description	Shimada, M., Goshima, T., Matsuo, H. et al. Essential role of autoactivation circuitry on Aurora B-mediated H2AX-pS121 in mitosis. Nat Commun 7, 12059 (2016). https://doi.org/10.1038/ncomms12059
Abstract	Proper deposition and activation of Aurora B at the centromere is critical for faithful chromosome segregation in mammals. However, the mechanistic basis for abrupt Aurora B kinase activation at the centromere has not yet been fully understood. We demonstrate here that Aurora B-mediated phosphorylation of histone H2AX at serine 121 (H2AX-pS121) promotes Aurora B autophosphorylation and is essential for proper chromosome segregation. Aurora B-mediated H2AX-pS121 is specifically detected at the centromere during mitosis. H2AX depletion results in a severe defect in activation and deposition of Aurora B at this locus. A phosphomimic mutant of H2AX at S121 interacts with activated Aurora B more efficiently than wild-type in vitro. Taken together, these results propose a model in which Aurora B-mediated H2AX-pS121 probably provide a platform for Aurora B autoactivation circuitry at centromeres and thus play a pivotal role in proper chromosome segregation.
Publisher	Nature Research
Source Title	Nature Communications
Volume (Issue)	7
Page	12059
Date of Issued	2016-07-08
Language	English
Handle URL	https://hdl.handle.net/11094/78587
eISSN	20411723
NCID	AA12645905
Relation.isIdenticalTo	DOI (Publisher Version) https://doi.org/10.1038/ncomms12059
Relation.isIdenticalTo	PMID 27389782
Access Rights	open access
CopyRight	© 2016 The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License.
oaire:version	Version of Record
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCTERMS.bibliographicCitation	Nature Communications. 2016, 7, p. 12059
DC.title	Essential role of autoactivation circuitry on Aurora B-mediated H2AX-pS121 in mitosis
DC.creator	Shimada, Midori
	Goshima, Takahiro
	Matsuo, Hiromi
	Johmura, Yoshikazu
	Haruta, Mayumi
	Murata, Kazuhiro
	Tanaka, Hiromitsu
	Ikawa, Masahito
	Nakanishi, Keiko
	Nakanishi, Makoto
DC.publisher	Nature Research
DC.language' scheme='DCTERMS.RFC1766	English
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2016-07-08
DC.identifier	https://doi.org/10.1038/ncomms12059
DC.description	Shimada, M., Goshima, T., Matsuo, H. et al. Essential role of autoactivation circuitry on Aurora B-mediated H2AX-pS121 in mitosis. Nat Commun 7, 12059 (2016). https://doi.org/10.1038/ncomms12059
DCTERMS.abstract	Proper deposition and activation of Aurora B at the centromere is critical for faithful chromosome segregation in mammals. However, the mechanistic basis for abrupt Aurora B kinase activation at the centromere has not yet been fully understood. We demonstrate here that Aurora B-mediated phosphorylation of histone H2AX at serine 121 (H2AX-pS121) promotes Aurora B autophosphorylation and is essential for proper chromosome segregation. Aurora B-mediated H2AX-pS121 is specifically detected at the centromere during mitosis. H2AX depletion results in a severe defect in activation and deposition of Aurora B at this locus. A phosphomimic mutant of H2AX at S121 interacts with activated Aurora B more efficiently than wild-type in vitro. Taken together, these results propose a model in which Aurora B-mediated H2AX-pS121 probably provide a platform for Aurora B autoactivation circuitry at centromeres and thus play a pivotal role in proper chromosome segregation.
DC.rights	© 2016 The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License.
DC.format	78587
citation_title	Essential role of autoactivation circuitry on Aurora B-mediated H2AX-pS121 in mitosis
citation_author	Shimada, Midori
	Goshima, Takahiro
	Matsuo, Hiromi
	Johmura, Yoshikazu
	Haruta, Mayumi
	Murata, Kazuhiro
	Tanaka, Hiromitsu
	Ikawa, Masahito
	Nakanishi, Keiko
	Nakanishi, Makoto
citation_publisher	Nature Research
citation_language	English
citation_date	2016-07-08
citation_journal_title	Nature Communications
citation_volume	7
citation_firstpage	12059
citation_doi	https://doi.org/10.1038/ncomms12059
citation_pmid	27389782
citation_public_url	https://hdl.handle.net/11094/78587

Essential role of autoactivation circuitry on Aurora B-mediated H2AX-pS121 in mitosis

Link to primary information

Item Information

Show details