Generation of Hprt-disrupted rat through mouse← rat ES chimeras

Isotani, Ayako; Yamagata, Kazuo; Okabe, Masaru et al.

Scientific Reports, 2016, 6, 24215

Number of Access:458（2025-08-22 01:08 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/78589

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Title
Generation of Hprt-disrupted rat through mouse← rat ES chimeras
Creator
NRID
1000020444523 1000020444523
CreatorName
Isotani, Ayako
NRID
1000010361312 1000010361312
CreatorName
Yamagata, Kazuo
NRID
1000030089875 1000030089875
CreatorName
Okabe, Masaru
NRID
1000020304066 1000020304066
ORCID
0000-0001-9859-6217 0000-0001-9859-6217
researchmap permalink
read0054910 read0054910
Scopus Author ID
16689054500 16689054500
CreatorName
Ikawa, Masahito
Description
Isotani, A., Yamagata, K., Okabe, M. et al. Generation of Hprt-disrupted rat through mouse←rat ES chimeras. Sci Rep 6, 24215 (2016). https://doi.org/10.1038/srep24215
Abstract
We established rat embryonic stem (ES) cell lines from a double transgenic rat line which harbours CAG-GFP for ubiquitous expression of GFP in somatic cells and Acr3-EGFP for expression in sperm (green body and green sperm: GBGS rat). By injecting the GBGS rat ES cells into mouse blastocysts and transplanting them into pseudopregnant mice, rat spermatozoa were produced in mouse← rat ES chimeras. Rat spermatozoa from the chimeric testis were able to fertilize eggs by testicular sperm extraction combined with intracytoplasmic sperm injection (TESE-ICSI). In the present paper, we disrupted rat hypoxanthine-guanine phosphoribosyl transferase (Hprt) gene in ES cells and produced a Hprt-disrupted rat line using the mouse← rat ES chimera system. The mouse← rat ES chimera system demonstrated the dual advantages of space conservation and a clear indication of germ line transmission in knockout rat production.
Publisher
Nature Research
Source Title
Scientific Reports
Volume (Issue)
6
Page
24215
Date of Issued
2016-04-11
Language
English
Handle URL
https://hdl.handle.net/11094/78589
eISSN
20452322
Relation.isIdenticalTo
DOI (Publisher Version)
https://doi.org/10.1038/srep24215
PMID
27062982
Access Rights
open access
CopyRight
© 2016 The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License.
oaire:version
Version of Record
Category
学術雑誌論文 Journal Article