CABYR is essential for fibrous sheath integrity and progressive motility in mouse spermatozoa

Young, Samantha A.M.; Miyata, Haruhiko; Satouh, Yuhkoh et al.

Journal of Cell Science, 2016, 129(23), 4379-4387

Number of Access:526（2025-08-22 01:08 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/78592

Link to primary information 

File Format Terms of use Size Views Date.Available Description information
JCellSci_129_23_4379 pdf None 3.07 MB 161 2021.02.08  

Item Information

Output File Export EndNote Basic Export Mendeley

Title
CABYR is essential for fibrous sheath integrity and progressive motility in mouse spermatozoa
Creator
CreatorName
Young, Samantha A.M.
NRID
1000050604732 1000050604732
researchmap permalink
hmiyata hmiyata
Scopus Author ID
37161837000 37161837000
ORCID
0000-0003-4758-5803 0000-0003-4758-5803
CreatorName
Miyata, Haruhiko
NRID
1000040545571 1000040545571
CreatorName
Satouh, Yuhkoh
CreatorName
Aitken, Robert John
CreatorName
Baker, Mark A.
NRID
1000020304066 1000020304066
ORCID
0000-0001-9859-6217 0000-0001-9859-6217
researchmap permalink
read0054910 read0054910
Scopus Author ID
16689054500 16689054500
CreatorName
Ikawa, Masahito
Subject
CABYR
CRISPR-Cas9
Fibrous sheath
Knockout
Spermatozoa
Abstract
Ca2+-binding tyrosine-phosphorylation-regulated protein (CABYR) has been implicated in sperm physiological function in several in vitro studies. It has also been implicated as a potential cause of and diagnostic tool in asthenozoospermic human males. CABYR is known to be localized to the fibrous sheath, an accessory structure in the flagellar principal piece. Utilizing the CRISPR-Cas9 technology, we have knocked out this gene inmice to understand its role inmale fertility. Cabyr-knockout male mice showed severe subfertility with a defect in sperm motility as well as a significant disorganization in the fibrous sheath. Further, abnormal configuration of doublet microtubules was observed in the Cabyr-knockout spermatozoa, suggesting that the fibrous sheath is important for the correct organization of the axoneme. Our results show that it is the role of CABYR in the formation of the fibrous sheath that is essential for male fertility.
Publisher
Company of Biologists Ltd
Source Title
Journal of Cell Science
Volume (Issue)
129(23)
Page
4379-4387
Date of Issued
2016
Language
English
Handle URL
https://hdl.handle.net/11094/78592
PISSN
00219533
eISSN
14779137
NCID
AA11720335
Relation.isIdenticalTo
DOI (Publisher Version)
https://doi.org/10.1242/jcs.193151
PMID
27802166
Access Rights
open access
oaire:version
Version of Record
Category
学術雑誌論文 Journal Article