Calcitonin Receptor Signaling Inhibits Muscle Stem Cells from Escaping the Quiescent State and the Niche

Yamaguchi, Masahiko; Watanabe, Yoko; Ohtani, Takuji; Uezumi, Akiyoshi; Mikami, Norihisa; Nakamura, Miki; Sato, Takahiko; Ikawa, Masahito; Hoshino, Mikio; Tsuchida, Kunihiro; Miyagoe, Yuko; Tsujikawa, Kazutake; Takeda, Shin'ichi; Yamamoto, Hiroshi; Fukada, So-ichiro

doi:https://doi.org/10.1016/j.celrep.2015.08.083

Calcitonin Receptor Signaling Inhibits Muscle Stem Cells from Escaping the Quiescent State and the Niche

Yamaguchi, Masahiko; Watanabe, Yoko; Ohtani, Takuji et al.

Cell Reports, 2015, 13(2), 302-314

Number of Access:2,284（2025-08-16 04:45 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/78600

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Title	Calcitonin Receptor Signaling Inhibits Muscle Stem Cells from Escaping the Quiescent State and the Niche
Creator	CreatorName Yamaguchi, Masahiko
	CreatorName Watanabe, Yoko
	CreatorName Ohtani, Takuji
	NRID 1000060434594 1000060434594 CreatorName Uezumi, Akiyoshi
	NRID 1000020710388 1000020710388 CreatorName Mikami, Norihisa
	CreatorName Nakamura, Miki
	NRID 1000030570775 1000030570775 CreatorName Sato, Takahiko
	NRID 1000020304066 1000020304066 ORCID 0000-0001-9859-6217 0000-0001-9859-6217 researchmap permalink read0054910 read0054910 Scopus Author ID 16689054500 16689054500 CreatorName Ikawa, Masahito
	NRID 1000070301273 1000070301273 CreatorName Hoshino, Mikio
	NRID 1000030281091 1000030281091 CreatorName Tsuchida, Kunihiro
	CreatorName Miyagoe, Yuko Creator.Alternative Suzuki, Yuko
	NRID 1000010207376 1000010207376 researchmap permalink read0014359 read0014359 Scopus Author ID 7004354760 7004354760 CreatorName Tsujikawa, Kazutake
	NRID 1000090171644 1000090171644 CreatorName Takeda, Shin'ichi
	NRID 1000050127312 1000050127312 CreatorName Yamamoto, Hiroshi
	NRID 1000020432445 1000020432445 ORCID 0000-0003-4051-5108 0000-0003-4051-5108 researchmap permalink mdx mdx Scopus Author ID 56020906000 56020906000 CreatorName Fukada, So-ichiro
Description	Masahiko Yamaguchi, Yoko Watanabe, Takuji Ohtani, Akiyoshi Uezumi, Norihisa Mikami, Miki Nakamura, Takahiko Sato, Masahito Ikawa, Mikio Hoshino, Kunihiro Tsuchida, Yuko Miyagoe-Suzuki, Kazutake Tsujikawa, Shin’ichi Takeda, Hiroshi Yamamoto, So-ichiro Fukada, Calcitonin Receptor Signaling Inhibits Muscle Stem Cells from Escaping the Quiescent State and the Niche, Cell Reports, Volume 13, Issue 2, 2015, Pages 302-314, ISSN 2211-1247, https://doi.org/10.1016/j.celrep.2015.08.083.
Abstract	Calcitonin receptor (Calcr) is expressed in adult muscle stem cells (muscle satellite cells [MuSCs]). To elucidate the role of Calcr, we conditionally depleted Calcr from adult MuSCs and found that impaired regeneration after muscle injury correlated with the decreased number of MuSCs in Calcr-conditional knockout (cKO) mice. Calcr signaling maintained MuSC dormancy via the cAMP-PKA pathway but had no impact on myogenic differentiation of MuSCs in an undifferentiated state. The abnormal quiescent state in Calcr-cKO mice resulted in a reduction of the MuSC pool by apoptosis. Furthermore, MuSCs were found outside their niche in Calcr-cKO mice, demonstrating cell relocation. This emergence from the sublaminar niche was prevented by the Calcr-cAMP-PKA and Calcr-cAMP-Epac pathways downstream of Calcr. Altogether, the findings demonstrated that Calcr exerts its effect specifically by keeping MuSCs in a quiescent state and in their location, maintaining the MuSC pool.
Publisher	Elsevier B.V.
Source Title	Cell Reports
Volume (Issue)	13(2)
Page	302-314
Date of Issued	2015-10-13
Language	English
Handle URL	https://hdl.handle.net/11094/78600
eISSN	22111247
Relation.isIdenticalTo	DOI (Publisher Version) https://doi.org/10.1016/j.celrep.2015.08.083
Relation.isIdenticalTo	PMID 26440893
Access Rights	open access
CopyRight	© 2015 The Authors. Published by Elsevier Inc. This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
oaire:version	Version of Record
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCTERMS.bibliographicCitation	Cell Reports. 2015, 13(2), p. 302-314
DC.title	Calcitonin Receptor Signaling Inhibits Muscle Stem Cells from Escaping the Quiescent State and the Niche
DC.creator	Yamaguchi, Masahiko
	Watanabe, Yoko
	Ohtani, Takuji
	Uezumi, Akiyoshi
	Mikami, Norihisa
	Nakamura, Miki
	Sato, Takahiko
	Ikawa, Masahito
	Hoshino, Mikio
	Tsuchida, Kunihiro
	Miyagoe, Yuko
	Tsujikawa, Kazutake
	Takeda, Shin'ichi
	Yamamoto, Hiroshi
	Fukada, So-ichiro
DC.creator	Suzuki, Yuko
DC.publisher	Elsevier B.V.
DC.language' scheme='DCTERMS.RFC1766	English
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2015-10-13
DC.identifier	https://doi.org/10.1016/j.celrep.2015.08.083
DC.description	Masahiko Yamaguchi, Yoko Watanabe, Takuji Ohtani, Akiyoshi Uezumi, Norihisa Mikami, Miki Nakamura, Takahiko Sato, Masahito Ikawa, Mikio Hoshino, Kunihiro Tsuchida, Yuko Miyagoe-Suzuki, Kazutake Tsujikawa, Shin’ichi Takeda, Hiroshi Yamamoto, So-ichiro Fukada, Calcitonin Receptor Signaling Inhibits Muscle Stem Cells from Escaping the Quiescent State and the Niche, Cell Reports, Volume 13, Issue 2, 2015, Pages 302-314, ISSN 2211-1247, https://doi.org/10.1016/j.celrep.2015.08.083.
DCTERMS.abstract	Calcitonin receptor (Calcr) is expressed in adult muscle stem cells (muscle satellite cells [MuSCs]). To elucidate the role of Calcr, we conditionally depleted Calcr from adult MuSCs and found that impaired regeneration after muscle injury correlated with the decreased number of MuSCs in Calcr-conditional knockout (cKO) mice. Calcr signaling maintained MuSC dormancy via the cAMP-PKA pathway but had no impact on myogenic differentiation of MuSCs in an undifferentiated state. The abnormal quiescent state in Calcr-cKO mice resulted in a reduction of the MuSC pool by apoptosis. Furthermore, MuSCs were found outside their niche in Calcr-cKO mice, demonstrating cell relocation. This emergence from the sublaminar niche was prevented by the Calcr-cAMP-PKA and Calcr-cAMP-Epac pathways downstream of Calcr. Altogether, the findings demonstrated that Calcr exerts its effect specifically by keeping MuSCs in a quiescent state and in their location, maintaining the MuSC pool.
DC.rights	© 2015 The Authors. Published by Elsevier Inc. This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
DC.format	78600
citation_title	Calcitonin Receptor Signaling Inhibits Muscle Stem Cells from Escaping the Quiescent State and the Niche
citation_author	Yamaguchi, Masahiko
	Watanabe, Yoko
	Ohtani, Takuji
	Uezumi, Akiyoshi
	Mikami, Norihisa
	Nakamura, Miki
	Sato, Takahiko
	Ikawa, Masahito
	Hoshino, Mikio
	Tsuchida, Kunihiro
	Miyagoe, Yuko
	Tsujikawa, Kazutake
	Takeda, Shin'ichi
	Yamamoto, Hiroshi
	Fukada, So-ichiro
citation_publisher	Elsevier B.V.
citation_language	English
citation_date	2015-10-13
citation_journal_title	Cell Reports
citation_volume	13
citation_issue	2
citation_firstpage	302
citation_lastpage	314
citation_doi	https://doi.org/10.1016/j.celrep.2015.08.083
citation_pmid	26440893
citation_public_url	https://hdl.handle.net/11094/78600

Calcitonin Receptor Signaling Inhibits Muscle Stem Cells from Escaping the Quiescent State and the Niche

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